Methods for the Stimulation of Lymphocytes. T Cell Mitogens and Polyclonal B Cell Activators. Cross-Linked Derivatives of Concanavalin A. Biochemical Changes Taking Place in Mitogen-Treated Lymphocytes. Allogeneic and Autologous Mixed Lymphocyte Reactions. Stimulation of Lymphocytes with Antibodies to Surface Membrane-Bound Antigen Receptors. Stimulation of Lymphocytes with Periodate of Neuraminidase plus Galactose Oxidase. Stimulation of Lymphocytes with Proteolytic Enzymes. Stimulation of Lymphocytes with Zinc Ions. Enzyme Immunoassay Using a Monoclonal Antibody against 5-Bromo-2-deoxyuridine for the Assessment of Lymphoid Cell Proliferation. T Cell Responses Studied in a Basal Serum-Free Medium. Chemically Defined Medium for the Growth of Lymphocytes. Proliferating Cell Nuclear Antigen: Cyclin. In Vitro Models and Assays of B and T Lymphocyte Differentiation and Function. Mouse Inbred and Cogenic Strains. Splenic Focus Assay. Direct and Indirect Plaque Assays. Protein A Plaque Assay. Poly(xL-lysine) Plaque Assay for the Measurement of Antigen-Activated Human B Lymphocytes. Growth of Antibody-Producing Cell Clones in Microcultures. Adherent Layer-Dependent Development of B Cell Progenitor in Semisolid Agar. Growth of B Cell Colonies in Double-Layer Agar Cultures. Culture in Liquid Medium of Single, Hapten-Specific, Antibody-Producing B Lymphocytes. Long-Term Culture of Murine Bone Marrow Precursors of B Lymphocytes. Cloning of Mitogen- and Antigen-Reactive B Lymphocytes on Filter Paper Disks: Phenotypic and Genotypic Analysis of B Cell Colonies. Production of Antibodies in Vitro in Cultures of Murine Lymphocytes. In Vitro Production of Antibody in Cultures of Human Peripheral Blood Lymphocytes. Purification and Characterization of Antigen-Binding Virgin and Memory B Cells. Murine T Cell Clones. Cloning of Human Alloreactive T Cells. Cloning with Antigens and Interleukin 2 of Murine T Lymphocytes Having Distinct Functions. Karyotypic Analysis of T Cell Clones and Hybrids. Generation of Terminal Deoxynucleotidyltransferase-Positive Lymphoid Precursor Cells in Vitro. Receptors on Lymphoid Cells. Receptors on Lymphoid Cells: An Overview. Strategies for the Isolation of Cell Surface Receptors of Lymphoid Cells. Nonpermeant Covalent Labels in Analytical Studies of Lymphocyte Membrane Proteins. Electron Spin Resonance Spectroscopy in the Study of Lymphoid Cell Receptors. Air-Driven Ultracentrifuge for Sedimentation Equilibrium and Binding Studies. Lectins in the Isolation of Receptors on Lymphocytes. Fluorescence Flow Cytometry in the Study of Lymphoid Cell Receptors. Photoaffinity Labeling in the Study of Lymphoid Cell /-Adrenergic Receptors. Membrane-Impermeant Cross-Linking Reagents: Application to the Study of the Cell Surface Receptor for IgE. Fcge Receptors. Immunoglobulin G Fc Receptors of Human Leukocytes. E-Rosette Receptor in Human Lymphocytes. Virus Receptors on Lymphoid Cells. C1q Receptor. Human Receptor for C3b/C4b: Complement Receptor Type I. The Complement Receptor Type 2 and Factor H Receptors. Human Complement Receptor Type 3. C5a Receptor. Antigen-Specific T Cell Receptors. Non-MHC-Restricted T Cell Antigen-Binding Proteins. Human Interleukin 2 Receptor. Receptors of Insulin and Growth Hormone on Lymphoid Cells. Lymphoid Receptors for Transferrin. Isolation of Genes Encoding Proteins of Immunological Importance. Author Index. Subject Index.
In this volume, in vitro models for the study of lymphoid cell functions and methods for the study of lymphoid cell receptors are presented. Lymphocyte in vitro transformation is discussed in the first section that describes methods for the in vitro stimulation of lymphocytes. Some special media for the study of lymphocyte transformation are also discussed. In vitro methods have been very useful for the understanding of the differentiation of lymphoid cells. The more commonly used of these methods are presented in the second section. The third section deals with the receptors of lymphoid cells.
Biochemists, immunologists, pharmacologists, toxicologists, clinical chemists, environmental scientists, industrial enzymologists, cell, developmental and molecular biologists, biomedical researchers and clinicians.
- No. of pages:
- © Academic Press 1987
- 28th December 1987
- Academic Press
- eBook ISBN:
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California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA
Vanderbilt University, Nashville, Tennessee, U.S.A.
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