Methods for the Stimulation of Lymphocytes. T Cell Mitogens and Polyclonal B Cell Activators. Cross-Linked Derivatives of Concanavalin A. Biochemical Changes Taking Place in Mitogen-Treated Lymphocytes. Allogeneic and Autologous Mixed Lymphocyte Reactions. Stimulation of Lymphocytes with Antibodies to Surface Membrane-Bound Antigen Receptors. Stimulation of Lymphocytes with Periodate of Neuraminidase plus Galactose Oxidase. Stimulation of Lymphocytes with Proteolytic Enzymes. Stimulation of Lymphocytes with Zinc Ions. Enzyme Immunoassay Using a Monoclonal Antibody against 5-Bromo-2-deoxyuridine for the Assessment of Lymphoid Cell Proliferation. T Cell Responses Studied in a Basal Serum-Free Medium. Chemically Defined Medium for the Growth of Lymphocytes. Proliferating Cell Nuclear Antigen: Cyclin. In Vitro Models and Assays of B and T Lymphocyte Differentiation and Function. Mouse Inbred and Cogenic Strains. Splenic Focus Assay. Direct and Indirect Plaque Assays. Protein A Plaque Assay. Poly(*xL-lysine) Plaque Assay for the Measurement of Antigen-Activated Human B Lymphocytes. Growth of Antibody-Producing Cell Clones in Microcultures. Adherent Layer-Dependent Development of B Cell Progenitor in Semisolid Agar. Growth of B Cell Colonies in Double-Layer Agar Cultures. Culture in Liquid Medium of Single, Hapten-Specific, Antibody-Producing B Lymphocytes. Long-Term Culture of Murine Bone Marrow Precursors of B Lymphocytes. Cloning of Mitogen- and Antigen-Reactive B Lymphocytes on Filter Paper Disks: Phenotypic and Genotypic Analysis of B Cell Colonies. Production of Antibodies in Vitro in Cultures of Murine Lymphocytes. In Vitro Production of Antibody
In this volume, in vitro models for the study of lymphoid cell functions and methods for the study of lymphoid cell receptors are presented. Lymphocyte in vitro transformation is discussed in the first section that describes methods for the in vitro stimulation of lymphocytes. Some special media for the study of lymphocyte transformation are also discussed. In vitro methods have been very useful for the understanding of the differentiation of lymphoid cells. The more commonly used of these methods are presented in the second section. The third section deals with the receptors of lymphoid cells.
Biochemists, immunologists, pharmacologists, toxicologists, clinical chemists, environmental scientists, industrial enzymologists, cell, developmental and molecular biologists, biomedical researchers and clinicians.
- No. of pages:
- © Academic Press 1987
- 28th December 1987
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY
Vanderbilt University, Nashville, Tennessee, U.S.A.
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA