Immunochemical Techniques, Part K: In Vitro Models of B and T Cell Function and Lymphoid Cell Receptors, Volume 150

1st Edition

Serial Volume Editors: Giovanni Di Sabato
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121820510
eBook ISBN: 9780080882673
Imprint: Academic Press
Published Date: 28th December 1987
Page Count: 823


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Table of Contents

Methods for the Stimulation of Lymphocytes. T Cell Mitogens and Polyclonal B Cell Activators. Cross-Linked Derivatives of Concanavalin A. Biochemical Changes Taking Place in Mitogen-Treated Lymphocytes. Allogeneic and Autologous Mixed Lymphocyte Reactions. Stimulation of Lymphocytes with Antibodies to Surface Membrane-Bound Antigen Receptors. Stimulation of Lymphocytes with Periodate of Neuraminidase plus Galactose Oxidase. Stimulation of Lymphocytes with Proteolytic Enzymes. Stimulation of Lymphocytes with Zinc Ions. Enzyme Immunoassay Using a Monoclonal Antibody against 5-Bromo-2-deoxyuridine for the Assessment of Lymphoid Cell Proliferation. T Cell Responses Studied in a Basal Serum-Free Medium. Chemically Defined Medium for the Growth of Lymphocytes. Proliferating Cell Nuclear Antigen: Cyclin. In Vitro Models and Assays of B and T Lymphocyte Differentiation and Function. Mouse Inbred and Cogenic Strains. Splenic Focus Assay. Direct and Indirect Plaque Assays. Protein A Plaque Assay. Poly(*xL-lysine) Plaque Assay for the Measurement of Antigen-Activated Human B Lymphocytes. Growth of Antibody-Producing Cell Clones in Microcultures. Adherent Layer-Dependent Development of B Cell Progenitor in Semisolid Agar. Growth of B Cell Colonies in Double-Layer Agar Cultures. Culture in Liquid Medium of Single, Hapten-Specific, Antibody-Producing B Lymphocytes. Long-Term Culture of Murine Bone Marrow Precursors of B Lymphocytes. Cloning of Mitogen- and Antigen-Reactive B Lymphocytes on Filter Paper Disks: Phenotypic and Genotypic Analysis of B Cell Colonies. Production of Antibodies in Vitro in Cultures of Murine Lymphocytes. In Vitro Production of Antibody


In this volume, in vitro models for the study of lymphoid cell functions and methods for the study of lymphoid cell receptors are presented. Lymphocyte in vitro transformation is discussed in the first section that describes methods for the in vitro stimulation of lymphocytes. Some special media for the study of lymphocyte transformation are also discussed. In vitro methods have been very useful for the understanding of the differentiation of lymphoid cells. The more commonly used of these methods are presented in the second section. The third section deals with the receptors of lymphoid cells.


Biochemists, immunologists, pharmacologists, toxicologists, clinical chemists, environmental scientists, industrial enzymologists, cell, developmental and molecular biologists, biomedical researchers and clinicians.


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© Academic Press 1987
Academic Press
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About the Serial Volume Editors

Giovanni Di Sabato Serial Volume Editor

Affiliations and Expertise

Vanderbilt University, Nashville, Tennessee, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA