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Immunochemical Techniques, Part E: Monoclonal Antibodies and General Immunoassay Methods - 1st Edition - ISBN: 9780121819927, 9780080882093

Immunochemical Techniques, Part E: Monoclonal Antibodies and General Immunoassay Methods, Volume 92

1st Edition

Editors in Chief: Nathan Kaplan Nathan Colowick
Serial Volume Editors: John Langone Helen Van Vunakis
eBook ISBN: 9780080882093
Imprint: Academic Press
Published Date: 28th March 1983
Page Count: 688


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Table of Contents

(Section Headings): Hybridoma Technology: Production of Monoclonal Antibodies with Selected Applications; Detection and Assessment of Monoclonal Antibodies. Immunoassay of Antigens and Antibodies: Labeling of Antigens and Antibodies; Separation Methods in Immunoassay; Immunoassay Methods; Data Analysis. Author Index. Subject Index.


FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein that appeared in Volume 73 of this series. Methods to enhance the efficiency of producing desired fusion products, to simplify the basic procedures involved, and to assess the specificity and other properties of monoclonal antibodies are discussed. Representative examples illustrate how a battery of monoclonal antibodies with different specificities can be used to study various immunochemical and biochemical problems. Other chapters cover additional procedures that can be used to label antigens and antibodies, separate antigen-antibody complexes, and process data. Some papers classified under one heading or contained in another volume of the series include methods that can be applied in other specific areas. For example, cytotoxicity tests described in Volume 93 may be used to screen supernatant fluids in hybridoma experiments if cytotoxic activity is a desirable property of the monoclonal antibody.


No. of pages:
© Academic Press 1983
28th March 1983
Academic Press
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

Nathan Kaplan

Nathan Colowick

Affiliations and Expertise

Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.

About the Serial Volume Editors

John Langone

Affiliations and Expertise

Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland, U.S.A.

Helen Van Vunakis

Affiliations and Expertise

Brandeis University, Waltham, Massachusetts, U.S.A.