Immunoassay - 1st Edition - ISBN: 9780121676353, 9780323137829


1st Edition

A Practical Guide

Authors: Author Unknown
eBook ISBN: 9780323137829
Imprint: Academic Press
Published Date: 16th September 1987
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During the last few years, immunoassay has gained tremendous popularity in clinical and research laboratories and has been applied to determine hormone, enzyme, protein, drug, and infectious agents. The aim of this book is to provide clinical laboratory personnel and students with an understanding of the principle of immunoassay and the production of reagents for immunoassay.

Table of Contents


  1. General Principle of Immunoassay I. Introduction II. Theoretical Basis of Immunoassay III. Optimization of Immunoassay IV. Heterogeneous Immunoassay Systems V. Homogeneous Immunoassay Systems References
  2. Production of Immunoassay Reagents I. Introduction II. Receptors or Binders III. Unlabeled Ligands—Calibrators IV. Labeled Ligands and Receptors V. Separation Techniques VI. Buffers References
  3. Practical Guide to Immunoassay Method Evaluation I. Introduction II. Protocol Outline III. Establishing Objectives and Preparation IV. Evaluation of Precision V. Standard Curves VI. Tracer VII. Sensitivity VIII. Evaluation of Accuracy IX. Antibody Characteristics X. Monitoring Reaction Conditions XI. Clinical Validation XII. Protocol Development: Summary References
  4. Clinical Validation of Immunoassays: A Well-Designed Approach to a Clinical Study I. Introduction II. Elements of a Well-Designed Study III. Choosing Decision Levels to Minimize Costs IV. Relation of New Tests to Existing Tests V. A Word about Normal Ranges VI. Summary VII. Illustrative Example: Apolipoprotein A-I Appendix: Preparation of ROC Curves References Selected Literature
  5. Data Reduction Techniques for Immunoassay I. Purpose of Chapter II. Introduction III. Interpolation Techniques of Data Reduction IV. Models and Statistical Approaches to Data Reduction V. Nonlinear Models in Immunoassay VI. Summary Appendix A: BASIC Program to Fit Four Parameter Model to Data by Nonlinear Least Squares Appendix B: BASIC Program for Point-to-Point Linear Interpolation References
  6. Immunoassays: Quality Control and Troubleshooting Part A: Quality Control: A Simple, Cost-Effective Approach I. Introduction


© Academic Press 1987
Academic Press
eBook ISBN:

About the Author

Author Unknown


@qu:The emphasis has been placed on 'practical'...the chapter on clinical validation of immunoassays is excellent. @source:--CLINICAL CHEMISTRY @qu:A valuable guide for clinical laboratory personnel and students. @source:--EXPERIMENTAL AND CLINICAL PHARMACLOGY

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