Immobilized Enzyme Principles - 1st Edition - ISBN: 9780120411016, 9781483215846

Immobilized Enzyme Principles, Volume 1

1st Edition

Applied Biochemistry and Bioengineering, Vol. 1

Editors: Lemuel B. Wingard Ephraim Katchalski-Katzir Leon Goldstein
eBook ISBN: 9781483215846
Imprint: Academic Press
Published Date: 28th December 1976
Page Count: 376
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Table of Contents


List of Contributors

Introduction to the Series

Preface

Immobilized Enzymes—A Survey

Text

References

The Chemistry of Enzyme Immobilization

I. Introduction

II. Immobilization of Enzymes by Adsorption

III. Immobilization of Enzymes by Entrapment

IV. Immobilization of Enzymes by Covalent Cross-Linking with Bi- or Multifunctional Reagents

V. Immobilization of Enzymes by Covalent Coupling to Polymeric Supports

VI. Concluding Remarks

References

Diffusion and Kinetics with Immobilized Enzymes

I. Introduction

II. Factors Affecting the Kinetics of Bound Enzymes

III. Heterogeneous Enzyme Kinetics

IV. Effect of External Diffusional Limitations

V. Effect of Internal Diffusional Limitations

VI. Analysis of Diffusional Effects and Determination of Kinetic Parameters from Experimental Data

VII. Dynamic Effect of Weak Acids and Bases on Heterogeneous Enzyme Kinetics

VIII. Electrostatic Effects on Bound-Enzyme Kinetics

IX. Coimmobilized Multienzyme Systems

X. Perspectives

List of Symbols

References

Design and Analysis of Immobilized-Enzyme Flow Reactors

I. Introduction

II. Enzyme Reactor Types and Their Choice

III. Idealized Enzyme Reactor Systems

IV. Steady-State Analysis of Mass Transfer and Biochemical Reaction in Enzyme Reactors

V. Mass Transfer-Kinetic Models for Specialized Systems: Tubular Reactors with Catalytically Active Wall/Hollow-Fiber Reactors

VI. Nonideal Fluid-Flow Patterns in Enzyme Reactors

VII. Transient Analysis of Immobilized-Enzyme Reactors

VIII. Modeling of Multienzyme Reactor Systems

IX. Combined Reaction and Separation Processes

X. Process Optimization of Enzyme Reactors

XI. Comparison of Relative Efficiencies of Several Reactor Designs

XII. Epilog

List of Symbols

References

Industrial Applications of Immobilized Enzymes and Immobilized Microbial Cells

I. Introduction

II. Industrial Applications of Immobilized Enzymes

III. Applications of Immobilized Microbial Cells

IV. Conclusions

References

Subject Index

Description

Applied Biochemistry and Bioengineering, Volume 1: Immobilized Enzyme Principles focuses on the utilization of immobilization techniques for the study and application of enzyme catalysts in a variety of potential end-uses.

This book emphasizes the preparation of enzyme-support systems, effects caused by the concurrent phenomena of enzyme-catalyzed reaction kinetics and mass transfer resistances, and how these reactions are incorporated into the design of enzyme-catalyzed reactor systems. The magnitude of the perturbation of the apparent kinetic parameters of an immobilized enzyme that could serve in principle as a measure of the effective concentrations of substrate, modifier, or inhibitor at the site of the enzymic reaction is also explained.

This volume is recommended for biological scientists and engineers, as well as researchers interested in the biochemical common denominator that causes the interaction of engineering practice and biological sciences for technological development.


Details

No. of pages:
376
Language:
English
Copyright:
© Academic Press 1976
Published:
Imprint:
Academic Press
eBook ISBN:
9781483215846

About the Editors

Lemuel B. Wingard Editor

Ephraim Katchalski-Katzir Editor

Leon Goldstein Editor