Immobilized Enzyme Principles

Immobilized Enzyme Principles

Applied Biochemistry and Bioengineering, Vol. 1

1st Edition - December 28, 1976

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  • Editors: Lemuel B. Wingard, Ephraim Katchalski-Katzir, Leon Goldstein
  • eBook ISBN: 9781483215846

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Applied Biochemistry and Bioengineering, Volume 1: Immobilized Enzyme Principles focuses on the utilization of immobilization techniques for the study and application of enzyme catalysts in a variety of potential end-uses. This book emphasizes the preparation of enzyme-support systems, effects caused by the concurrent phenomena of enzyme-catalyzed reaction kinetics and mass transfer resistances, and how these reactions are incorporated into the design of enzyme-catalyzed reactor systems. The magnitude of the perturbation of the apparent kinetic parameters of an immobilized enzyme that could serve in principle as a measure of the effective concentrations of substrate, modifier, or inhibitor at the site of the enzymic reaction is also explained. This volume is recommended for biological scientists and engineers, as well as researchers interested in the biochemical common denominator that causes the interaction of engineering practice and biological sciences for technological development.

Table of Contents

  • List of Contributors

    Introduction to the Series


    Immobilized Enzymes—A Survey



    The Chemistry of Enzyme Immobilization

    I. Introduction

    II. Immobilization of Enzymes by Adsorption

    III. Immobilization of Enzymes by Entrapment

    IV. Immobilization of Enzymes by Covalent Cross-Linking with Bi- or Multifunctional Reagents

    V. Immobilization of Enzymes by Covalent Coupling to Polymeric Supports

    VI. Concluding Remarks


    Diffusion and Kinetics with Immobilized Enzymes

    I. Introduction

    II. Factors Affecting the Kinetics of Bound Enzymes

    III. Heterogeneous Enzyme Kinetics

    IV. Effect of External Diffusional Limitations

    V. Effect of Internal Diffusional Limitations

    VI. Analysis of Diffusional Effects and Determination of Kinetic Parameters from Experimental Data

    VII. Dynamic Effect of Weak Acids and Bases on Heterogeneous Enzyme Kinetics

    VIII. Electrostatic Effects on Bound-Enzyme Kinetics

    IX. Coimmobilized Multienzyme Systems

    X. Perspectives

    List of Symbols


    Design and Analysis of Immobilized-Enzyme Flow Reactors

    I. Introduction

    II. Enzyme Reactor Types and Their Choice

    III. Idealized Enzyme Reactor Systems

    IV. Steady-State Analysis of Mass Transfer and Biochemical Reaction in Enzyme Reactors

    V. Mass Transfer-Kinetic Models for Specialized Systems: Tubular Reactors with Catalytically Active Wall/Hollow-Fiber Reactors

    VI. Nonideal Fluid-Flow Patterns in Enzyme Reactors

    VII. Transient Analysis of Immobilized-Enzyme Reactors

    VIII. Modeling of Multienzyme Reactor Systems

    IX. Combined Reaction and Separation Processes

    X. Process Optimization of Enzyme Reactors

    XI. Comparison of Relative Efficiencies of Several Reactor Designs

    XII. Epilog

    List of Symbols


    Industrial Applications of Immobilized Enzymes and Immobilized Microbial Cells

    I. Introduction

    II. Industrial Applications of Immobilized Enzymes

    III. Applications of Immobilized Microbial Cells

    IV. Conclusions


    Subject Index

Product details

  • No. of pages: 376
  • Language: English
  • Copyright: © Academic Press 1976
  • Published: December 28, 1976
  • Imprint: Academic Press
  • eBook ISBN: 9781483215846

About the Editors

Lemuel B. Wingard

Ephraim Katchalski-Katzir

Leon Goldstein

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