This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Key Features

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

    Table of Contents

    Part A: Techniques

    1. Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells
    2. Stanley W. Botchway, Pamela Reynolds, Anthony W. Parker, Peter O’Neill

    3. A cell biologist’s guide to high resolution imaging
    4. Graeme Ball, Richard M Parton, Russell S Hamilton and Ilan Davis

    5. Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
    6. André E.P. Bastos, Silvia Scolari, Martin Stöckl, Rodrigo F.M. de Almeida

    7. Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells
    8. Gufeng Wang and Ning Fang

    9. Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity
    10. Gert-Jan Bakker, Volker Andresen, Robert M. Hoffman, Peter Friedl

    11. Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)
    12. Inmaculada M González-González, Frédéric Jaskolski, Yves Goldberg, Michael C. Ashby and Jeremy M. Henley

    13. Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies
    14. Rosa Perestrelo, Francisco Albuquerque, Sílvia M. Rocha, José S. Câmara

    15. Green to red photo-convertible mEosFP aided live imaging in plants
    16. Jaideep Mathur, Sarah Griffiths, Kiah Barton and Martin H. Schattat

    17. Methods for Cell and Particle Tracking
    18. Erik Meijering, Oleh Dzyubachyk, Ihor Smal

    19. Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures
    20. Alexander A. Mironov and Galina V. Beznoussenko

    21. Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell P


    No. of pages:
    © 2012
    Academic Press
    Print ISBN:
    Electronic ISBN: