Imaging and Spectroscopic Analysis of Living Cells

Imaging and Spectroscopic Analysis of Living Cells

Live Cell Imaging of Cellular Elements and Functions

1st Edition - February 1, 2012

Write a review

  • Editor: P. Michael Conn
  • eBook ISBN: 9780123919076
  • Hardcover ISBN: 9780123884480

Purchase options

Purchase options
DRM-free (PDF, EPub, Mobi)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Key Features

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods


Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

Table of Contents

  • Part A: Organelles and Cellular Structure

    1. Monitoring Cytoskeletal Dynamics in Living Neurons using Fluorescence Photoactivation
    2. Anne Gauthier-Kemper, Carina Weissmann, Hans-Jürgen Reyher, and Roland Brandt

    3. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays
    4. Roy Edward

    5. Methods to measure actin treadmilling rate in dendritic spines
    6. Mikko Koskinen, Enni Bertling, and Pirta Hotulainen

    7. Live Cell Imaging of Clathrin Coats
    8. Comert Kural and Tom Kirchhausen

    9. Imaging the Mitotic Spindle
    10. Paul S. Maddox, Anne-Marie Ladouceur, Rajesh Ranjan, Jonas Dorn, Hery Ratsima, Damien D’Amours, and Amy S. Maddox

    11. Modeling of spatial intracellular signaling events in neurons
    12. Wendy C. Wenderski and Susana R. Neves

    13. Imaging chromosome dynamics in meiosis in plants
    14. Moira J. Sheehan, and Wojciech P. Pawlowski

    15. Analysing lysosomes in live cells
    16. Paul R. Pryor

    17. Detection and Quantification of Biomolecular Association in Living Cells using Single Molecule Microscopy
    18. Mario Brameshuber,and Gerhard J. Schütz

    19. Visualizing cardiac ion channel trafficking pathways
    20. James W. Smyth and Robin M. Shaw

    21. Live Cell Imaging of the Cytoskeleton
    22. Eve G. Stringham, Nancy Marcus-Gueret, Laura Ramsay and Kristopher L. Schmidt

    23. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms
    24. Naoki Watanabe

    25. Analysis of cell dispersion and migration by video-microscopy
    26. Bonnomet Arnaud, Terryn Christine, Cutrona Jérôme, Jonquet Antoine, Birembaut Philippe, and Zahm Jean-Marie

      Part B: Molecules and Ions

    27. Real Time Qualitative and Quantitative Glut4 Translocation Assay
    28. Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat

    29. Advanced imaging of cellular signalling events
    30. JMarek Cebecauer, Jana Humpolíčková and Jerémie Rossy

    31. Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy
    32. Subhasri Ghosh, Suvrajit Saha, Debanjan Goswami, Sameera Bilgrami and Satyajit Mayor

    33. Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells
    34. Harsha E. Rajapakse and Lawrence W. Miller

    35. Imaging of corticosteroid receptors in live cells
    36. Mayumi Nishi

    37. Investigating second messenger signaling in vivo
    38. Rüdiger Rudolf, Mathias Hafner, and Marco Mongillo

    39. Probes for Intracellular RNA Imaging In Live Cells
    40. Philip J. Santangelo, Eric Alonas, Jeenah Jung, Aaron W. Lifland, and Chiara Zurla

    41. Imaging the Glycome in Living Systems
    42. Boyangzi Li, Feiyan Mock and Peng Wu

    43. Intracellular Magnesium Detection by Fluorescent Indicators
    44. Valentina Trapani, Monika Schweigel-Röntgen, Achille Cittadini and Federica I. Wolf

    45. Illuminating mobile zinc with fluorescence: From cuvettes to live cells and tissues
    46. Zhen Huang and Stephen J. Lippard

    47. Quantitative fluorescent live cell imaging of intracellular Ca2+ and H+ ions in malaria parasites

              Petra Rohrbach

Product details

  • No. of pages: 576
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: February 1, 2012
  • Imprint: Academic Press
  • eBook ISBN: 9780123919076
  • Hardcover ISBN: 9780123884480

About the Serial Volume Editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Affiliations and Expertise

Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center, TX, USA

Ratings and Reviews

Write a review

There are currently no reviews for "Imaging and Spectroscopic Analysis of Living Cells"