Description

This volume of Methods in Enzymology is the second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Key Features

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

    Table of Contents

    Part A: Organelles and Cellular Structure

    1. Monitoring Cytoskeletal Dynamics in Living Neurons using Fluorescence Photoactivation
    2. Anne Gauthier-Kemper, Carina Weissmann, Hans-Jürgen Reyher, and Roland Brandt

    3. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays
    4. Roy Edward

    5. Methods to measure actin treadmilling rate in dendritic spines
    6. Mikko Koskinen, Enni Bertling, and Pirta Hotulainen

    7. Live Cell Imaging of Clathrin Coats
    8. Comert Kural and Tom Kirchhausen

    9. Imaging the Mitotic Spindle
    10. Paul S. Maddox, Anne-Marie Ladouceur, Rajesh Ranjan, Jonas Dorn, Hery Ratsima, Damien D’Amours, and Amy S. Maddox

    11. Modeling of spatial intracellular signaling events in neurons
    12. Wendy C. Wenderski and Susana R. Neves

    13. Imaging chromosome dynamics in meiosis in plants
    14. Moira J. Sheehan, and Wojciech P. Pawlowski

    15. Analysing lysosomes in live cells
    16. Paul R. Pryor

    17. Detection and Quantification of Biomolecular Association in Living Cells using Single Molecule Microscopy
    18. Mario Brameshuber,and Gerhard J. Schütz

    19. Visualizing cardiac ion channel trafficking pathways
    20. James W. Smyth and Robin M. Shaw

    21. Live Cell Imaging of the Cytoskeleton
    22. Eve G. Stringham, Nancy Marcus-Gueret, Laura Ramsay and Kristopher L. Schmidt

    23. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms
    24. Naoki Watanabe

    25. Analysis of cell dispersion and migration by video-microscopy
    26. Bonnomet Arnaud, Terryn C

    Details

    No. of pages:
    576
    Language:
    English
    Copyright:
    © 2012
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780123884480
    Electronic ISBN:
    9780123919076