Description

This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Key Features

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

Table of Contents

Part A: Organelles and Cellular Structure

  1. Monitoring Cytoskeletal Dynamics in Living Neurons using Fluorescence Photoactivation
  2. Anne Gauthier-Kemper, Carina Weissmann, Hans-Jürgen Reyher, and Roland Brandt

  3. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays
  4. Roy Edward

  5. Methods to measure actin treadmilling rate in dendritic spines
  6. Mikko Koskinen, Enni Bertling, and Pirta Hotulainen

  7. Live Cell Imaging of Clathrin Coats
  8. Comert Kural and Tom Kirchhausen

  9. Imaging the Mitotic Spindle
  10. Paul S. Maddox, Anne-Marie Ladouceur, Rajesh Ranjan, Jonas Dorn, Hery Ratsima, Damien D’Amours, and Amy S. Maddox

  11. Modeling of spatial intracellular signaling events in neurons
  12. Wendy C. Wenderski and Susana R. Neves

  13. Imaging chromosome dynamics in meiosis in plants
  14. Moira J. Sheehan, and Wojciech P. Pawlowski

  15. Analysing lysosomes in live cells
  16. Paul R. Pryor

  17. Detection and Quantification of Biomolecular Association in Living Cells using Single Molecule Microscopy
  18. Mario Brameshuber,and Gerhard J. Schütz

  19. Visualizing cardiac ion channel trafficking pathways
  20. James W. Smyth and Robin M. Shaw

  21. Live Cell Imaging of the Cytoskeleton
  22. Eve G. Stringham, Nancy Marcus-Gueret, Laura Ramsay and Kristopher L. Schmidt

  23. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms
  24. Naoki Watanabe

  25. Analysis of cell dispersion and migration by video-microscopy
  26. Bonnomet Arnaud, Terryn C

Details

No. of pages:
576
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123919076
Print ISBN:
9780123884480

About the serial-volume-editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve