Hydrogen Peroxide and Cell Signaling, Part C , Volume 528

1st Edition

Serial Volume Editors: Enrique Cadenas
Series Volume Editors: Lester Packer
Hardcover ISBN: 9780124058811
eBook ISBN: 9780124055407
Imprint: Academic Press
Published Date: 1st September 2013
Page Count: 384
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Table of Contents

Series Page

METHODS IN ENZYMOLOGY

Contributors

Preface

Volume in Series

Section I: H2O2 Regulation of Cell Signaling

Chapter One. The Biological Chemistry of Hydrogen Peroxide

1 Introduction

2 Chemical Properties

3 Antioxidant Defenses Against H2O2

4 Kinetics and Identification of Biological Targets for H2O2

5 Transmission of Redox Signals Initiated by H2O2

6 Diffusion Distances and Compartmentalization

7 Biological Detection of H2O2

8 Conclusion

References

Chapter Two. Reactive Oxygen Species in the Activation of MAP Kinases

1 Introduction

2 Reactive Oxygen Species

3 Mitogen-Activated Protein Kinases

4 Roles of ROS in MAPK Activation

5 Summary

Acknowledgment

References

Chapter Three. Hydrogen Peroxide Signaling Mediator in the Activation of p38 MAPK in Vascular Endothelial Cells

1 Introduction

2 Materials and Methods

References

Chapter Four. In Vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes

1 Introduction

2 Isolation and Culture of Adult Mouse Ventricular Cardiac Myocytes

3 Live Cell Imaging of Cardiac Myocytes

4 Imaging Intracellular NO with Cu2(FL2E) Dye

5 Production and In Vivo Expression of Lentivirus Expressing the HyPer2 H2O2 Biosensor

6 Imaging Intracellular H2O2 in Cardiac Myocytes and Endothelial Cells Expressing HyPer2

Acknowledgments

References

Chapter Five. Methods for Studying Oxidative Regulation of Protein Kinase C

1 Introduction

2 Materials

3 Direct Oxidative Modification of PKC Isoenzymes by H2O2


Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.


Details

No. of pages:
384
Language:
English
Copyright:
© Academic Press 2013
Published:
Imprint:
Academic Press
eBook ISBN:
9780124055407
Hardcover ISBN:
9780124058811

Reviews

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.


About the Serial Volume Editors

Enrique Cadenas Serial Volume Editor

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases.

Affiliations and Expertise

Pharmacology & Pharmaceutical Sciences, School of Pharmacy, University of Southern California, USA

About the Series Volume Editors

Lester Packer Series Volume Editor

Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.

Affiliations and Expertise

Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA