Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

Series Page

METHODS IN ENZYMOLOGY

Contributors

Preface

Volume in Series

Section I: H2O2 Regulation of Cell Signaling

Chapter One. The Biological Chemistry of Hydrogen Peroxide

1 Introduction

2 Chemical Properties

3 Antioxidant Defenses Against H2O2

4 Kinetics and Identification of Biological Targets for H2O2

5 Transmission of Redox Signals Initiated by H2O2

6 Diffusion Distances and Compartmentalization

7 Biological Detection of H2O2

8 Conclusion

References

Chapter Two. Reactive Oxygen Species in the Activation of MAP Kinases

1 Introduction

2 Reactive Oxygen Species

3 Mitogen-Activated Protein Kinases

4 Roles of ROS in MAPK Activation

5 Summary

Acknowledgment

References

Chapter Three. Hydrogen Peroxide Signaling Mediator in the Activation of p38 MAPK in Vascular Endothelial Cells

1 Introduction

2 Materials and Methods

References

Chapter Four. In Vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes

1 Introduction

2 Isolation and Culture of Adult Mouse Ventricular Cardiac Myocytes

3 Live Cell Imaging of Cardiac Myocytes

4 Imaging Intracellular NO with Cu2(FL2E) Dye

5 Production and In Vivo Expression of Lentivirus Expressing the HyPer2 H2O2 Biosensor

6 Imaging Intracellular H2O2 in Cardiac Myocytes and Endothelial Cells Expressing HyPer2

Acknowledgments

References

Chapter Five. Methods for Studying Oxidative Regulation of Protein Kinase C

1 Introduction

2 Materials

3 Direct Oxidative Modification of PKC Isoenzymes by H2O2

Details

No. of pages:
384
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Electronic ISBN:
9780124055407
Print ISBN:
9780124058811

About the serial-volume-editor

Enrique Cadenas

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases.