Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

Series Page

METHODS IN ENZYMOLOGY

Contributors

Preface

Methods in Enzymology

Section I: H2O2 Metabolism: Determination of a Cellular Steady-State

Chapter One. The Cellular Steady-State of H2O2: Latency Concepts and Gradients

1 Introduction

2 Experimental Components and Considerations When Measuring the H2O2 Gradient in S. cerevisiae Cells

3 Experimental Components and Considerations When Measuring the H2O2 Gradient in Mammalian Cell Lines

4 Data Handling/Processing

5 Summary

Acknowledgments

References

Chapter Two. Evaluating Peroxiredoxin Sensitivity Toward Inactivation by Peroxide Substrates

1 Introduction

2 Materials

3 Measuring Inactivation Sensitivity by Steady-State NADPH-Linked Assays

4 Measuring Inactivation Sensitivity by Multiturnover Cycling with ROOH and DTT Followed by Mass Spectrometry Analysis

5 Measuring Inactivation Sensitivity of Prx1/AhpC Prxs Under Single Turnover Conditions Followed by Gel Electrophoresis

6 Conclusions/Summary

Acknowledgment

References

Chapter Three. Peroxiredoxins as Preferential Targets in H2O2-Induced Signaling

1 Introduction

2 Reaction of H2O2 with Cellular Thiols

3 H2O2 Diffusion Versus Reaction with Cellular Thiols

4 Sulfenic Acids as Signal Transduction Intermediates

5 Prxs as Preferential Targets for H2O2

6 Prxs as Primary H2O2 Sensors and Transducers

7 Prx–Protein Interactions are Needed to Transmit the Signal

8 Posttranslational Regulation of Prxs

9 Summary

Acknowledgments

References

Chapter Four. Selenium in the Redox Regulation o

Details

No. of pages:
376
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780124058828
Electronic ISBN:
9780124055414