Hydrogen Peroxide and Cell Signaling, Part B, Volume 527

1st Edition

Serial Volume Editors: Lester Packer Enrique Cadenas
Hardcover ISBN: 9780124058828
eBook ISBN: 9780124055414
Imprint: Academic Press
Published Date: 15th July 2013
Page Count: 376
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Table of Contents

Series Page

METHODS IN ENZYMOLOGY

Contributors

Preface

Methods in Enzymology

Section I: H2O2 Metabolism: Determination of a Cellular Steady-State

Chapter One. The Cellular Steady-State of H2O2: Latency Concepts and Gradients

1 Introduction

2 Experimental Components and Considerations When Measuring the H2O2 Gradient in S. cerevisiae Cells

3 Experimental Components and Considerations When Measuring the H2O2 Gradient in Mammalian Cell Lines

4 Data Handling/Processing

5 Summary

Acknowledgments

References

Chapter Two. Evaluating Peroxiredoxin Sensitivity Toward Inactivation by Peroxide Substrates

1 Introduction

2 Materials

3 Measuring Inactivation Sensitivity by Steady-State NADPH-Linked Assays

4 Measuring Inactivation Sensitivity by Multiturnover Cycling with ROOH and DTT Followed by Mass Spectrometry Analysis

5 Measuring Inactivation Sensitivity of Prx1/AhpC Prxs Under Single Turnover Conditions Followed by Gel Electrophoresis

6 Conclusions/Summary

Acknowledgment

References

Chapter Three. Peroxiredoxins as Preferential Targets in H2O2-Induced Signaling

1 Introduction

2 Reaction of H2O2 with Cellular Thiols

3 H2O2 Diffusion Versus Reaction with Cellular Thiols

4 Sulfenic Acids as Signal Transduction Intermediates

5 Prxs as Preferential Targets for H2O2

6 Prxs as Primary H2O2 Sensors and Transducers

7 Prx–Protein Interactions are Needed to Transmit the Signal

8 Posttranslational Regulation of Prxs

9 Summary

Acknowledgments

References

Chapter Four. Selenium in the Redox Regulation o


Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.


Details

No. of pages:
376
Language:
English
Copyright:
© Academic Press 2013
Published:
Imprint:
Academic Press
eBook ISBN:
9780124055414
Hardcover ISBN:
9780124058828

About the Serial Volume Editors

Lester Packer Serial Volume Editor

Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.

Affiliations and Expertise

Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA

Enrique Cadenas Serial Volume Editor

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases.

Affiliations and Expertise

Pharmacology & Pharmaceutical Sciences, School of Pharmacy, University of Southern California, USA