Hydrogen Peroxide and Cell Signaling, Part A, Volume 526

1st Edition

Serial Volume Editors: Lester Packer Enrique Cadenas
Hardcover ISBN: 9780124058835
eBook ISBN: 9780124055421
Imprint: Academic Press
Published Date: 5th July 2013
Page Count: 328
Tax/VAT will be calculated at check-out
Compatible Not compatible
VitalSource PC, Mac, iPhone & iPad Amazon Kindle eReader
ePub & PDF Apple & PC desktop. Mobile devices (Apple & Android) Amazon Kindle eReader
Mobi Amazon Kindle eReader Anything else

Institutional Access

Table of Contents

Series Page




Methods in Enzymology

Section 1: Hydrogen Peroxide Detection Methods

Chapter One. Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to Light


1 Introduction

2 Amplex Red Assay and Its Possible Artifacts due to Light Exposure

3 Experimental Considerations



Chapter Two. Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems

1 Introduction

2 Fluorescent Boronate-Based Hydrogen Peroxide Probes

3 Examples of Probes and Their Usage

4 Imaging Endogenous H2O2 Using Fluorescent Probes

5 Conclusions



Chapter Three. Visualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent Probe

1 Introduction

2 Experimental Design

3 Materials

4 Procedure



Chapter Four. In Vivo Imaging of H2O2 Production in Drosophila

1 Introduction

2 Materials

3 Methods

4 Image and Data Analysis

5 Data Interpretation



Chapter Five. Single Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell Imaging

1 Introduction

2 A Guide to Single Fluorescent Probe Distinguishes H2O2 and NO

3 Fluorescence Imaging of Endogenously Produced H2O2 and NO in RAW 264.7 Macrophages Cells

4 Summary



Chapter Six. Electrochemical Biosensors for On-Chip Detection of Oxidative Stress from Cells

1 Introduction

2 Experimental Components and Procedures

3 Met


This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.


No. of pages:
© Academic Press 2013
Academic Press
eBook ISBN:
Hardcover ISBN:

About the Serial Volume Editors

Lester Packer Serial Volume Editor

Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.

Affiliations and Expertise

Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA

Enrique Cadenas Serial Volume Editor

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases.

Affiliations and Expertise

Pharmacology & Pharmaceutical Sciences, School of Pharmacy, University of Southern California, USA