Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

Series Page

METHODS IN ENZYMOLOGY

Contributors

Preface

Methods in Enzymology

Section 1: Hydrogen Peroxide Detection Methods

Chapter One. Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to Light

Abbreviations

1 Introduction

2 Amplex Red Assay and Its Possible Artifacts due to Light Exposure

3 Experimental Considerations

Acknowledgments

References

Chapter Two. Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems

1 Introduction

2 Fluorescent Boronate-Based Hydrogen Peroxide Probes

3 Examples of Probes and Their Usage

4 Imaging Endogenous H2O2 Using Fluorescent Probes

5 Conclusions

Acknowledgments

References

Chapter Three. Visualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent Probe

1 Introduction

2 Experimental Design

3 Materials

4 Procedure

Acknowledgments

References

Chapter Four. In Vivo Imaging of H2O2 Production in Drosophila

1 Introduction

2 Materials

3 Methods

4 Image and Data Analysis

5 Data Interpretation

Acknowledgments

References

Chapter Five. Single Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell Imaging

1 Introduction

2 A Guide to Single Fluorescent Probe Distinguishes H2O2 and NO

3 Fluorescence Imaging of Endogenously Produced H2O2 and NO in RAW 264.7 Macrophages Cells

4 Summary

Acknowledgments

References

Chapter Six. Electrochemical Biosensors for On-Chip Detection of Oxidative Stress from Cells

1 Introduction

2 Experimental Components and Procedures

3 Met

Details

No. of pages:
328
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780124058835
Electronic ISBN:
9780124055421