Description

This volume is the first comprehensive compilation of methods for the assay and purification of enzymes involved in the utilization of reduced one-carbon compounds and higher hydrocarbons. In the last decade there has been an explosion of interest in the organisms that grow on one-carbon compounds and in those that grow on higher hydrocarbons. This is due to the commercial interest in the unique enzymes involved in these specialty metabolic pathways and to a growing understanding of the important role these organisms play in carbon cycling in nature. More recent interest in the use of these organisms and their enzymes for detoxification of hazardous waste has once again put the spotlight on these unique microorganisms. This volume will prove to be a valuable source for researchers in academia, government, and industry alike.

Readership

Biochemists; basic research and industrial microbiologists (including those working at oil companies); environmental, organic, analytical, and microbial chemists.

Table of Contents

Hydrocarbons and Related Compounds: Enzymes of Alkane and Alkene Utilization: S.W. May and A.G. Katopodis, Hydrocarbon Monooxygenase System of Pseudomonas oleovorans. W.R. Finnerty, Assay Methods for Long-Chain Alkane Oxidation in Acinetobacter. W.R. Finnerty, Primary Alcohol Dehydrogenases from Acinetobacter. W.R. Finnerty, Aldehyde Dehydrogenases from Acinetobacter. W. Ashraf and J.C. Murrell, Propane-Specific Alcohol Dehydrogenase from Rhodococcus rhodochrous PNKb1. J.C. Murrell and W. Ashraf, Cell-Free Assay Methods for Enzymes of Propane Utilization. B.W. Groen and J.A. Duine, Quinoprotein Alcohol Dehydrogenase from Pseudomonas aeruginosa and Quinhemoprotein Alcohol Dehydrogenase from Comamonas testosteroni (Pseudomonas testosteroni). Enzymes Involved in Cyclic and Aromatic Hydrocarbon Utilization: L.P. Wackett, Toluene Dioxygenase from Pseudomonas putida F1. B.D. Ensley and B.E. Haigler, Naphthalene Dioxygenase from Pseudomonas NCIB 9816. P.J. Geary, J.R. Mason, and C.L. Joannou, Benzene Dioxygenase from Pseudomonas putida ML2 (NCIB 12190). C.J. Batie and D.P. Ballou, Phthalate Dioxygenase. P.W. Trudgill, Cyclohexanone 1,2-Monooxygenase from Acinetobacter NCIMB 9871. P.W. Trudgill, Cyclohexanone 1,2-Monooxygenase from Pseudomonas NCIMB 9872. J.W. Whittaker, A.M. Orville, and J.D. Lipscomb, Protocatechuate 3,4-Dioxygenase from Brevibacterium fuscum. D.M. Arciero, A.M. Orville, and J.D. Lipscomb, Protocatechuate 4,5-Dioxygenase from Pseudomonas testosteroni. S.A. Wolgel, and J.D. Lipscomb, Protocatechuate 2,3-Dioxygenase from Bacillus macerans. M.R. Harp

Details

No. of pages:
504
Language:
English
Copyright:
© 1990
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883052
Print ISBN:
9780121820893

About the serial-volume-editor

Mary Lidstrom

Affiliations and Expertise

Keck Laboratories, California Institute of Technology, Pasadena, California, U.S.A.

Reviews

@from:Praise for the Volume @qu:"Recommeded for all individuals with an interest with eicosanoids and lipid mediators." @source:--AMERICAN JOURNAL OF PHYSIOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY