This volume is the first comprehensive compilation of methods for the assay and purification of enzymes involved in the utilization of reduced one-carbon compounds and higher hydrocarbons. In the last decade there has been an explosion of interest in the organisms that grow on one-carbon compounds and in those that grow on higher hydrocarbons. This is due to the commercial interest in the unique enzymes involved in these specialty metabolic pathways and to a growing understanding of the important role these organisms play in carbon cycling in nature. More recent interest in the use of these organisms and their enzymes for detoxification of hazardous waste has once again put the spotlight on these unique microorganisms. This volume will prove to be a valuable source for researchers in academia, government, and industry alike.


Biochemists; basic research and industrial microbiologists (including those working at oil companies); environmental, organic, analytical, and microbial chemists.

Table of Contents

Hydrocarbons and Related Compounds: Enzymes of Alkane and Alkene Utilization: S.W. May and A.G. Katopodis, Hydrocarbon Monooxygenase System of Pseudomonas oleovorans. W.R. Finnerty, Assay Methods for Long-Chain Alkane Oxidation in Acinetobacter. W.R. Finnerty, Primary Alcohol Dehydrogenases from Acinetobacter. W.R. Finnerty, Aldehyde Dehydrogenases from Acinetobacter. W. Ashraf and J.C. Murrell, Propane-Specific Alcohol Dehydrogenase from Rhodococcus rhodochrous PNKb1. J.C. Murrell and W. Ashraf, Cell-Free Assay Methods for Enzymes of Propane Utilization. B.W. Groen and J.A. Duine, Quinoprotein Alcohol Dehydrogenase from Pseudomonas aeruginosa and Quinhemoprotein Alcohol Dehydrogenase from Comamonas testosteroni (Pseudomonas testosteroni). Enzymes Involved in Cyclic and Aromatic Hydrocarbon Utilization: L.P. Wackett, Toluene Dioxygenase from Pseudomonas putida F1. B.D. Ensley and B.E. Haigler, Naphthalene Dioxygenase from Pseudomonas NCIB 9816. P.J. Geary, J.R. Mason, and C.L. Joannou, Benzene Dioxygenase from Pseudomonas putida ML2 (NCIB 12190). C.J. Batie and D.P. Ballou, Phthalate Dioxygenase. P.W. Trudgill, Cyclohexanone 1,2-Monooxygenase from Acinetobacter NCIMB 9871. P.W. Trudgill, Cyclohexanone 1,2-Monooxygenase from Pseudomonas NCIMB 9872. J.W. Whittaker, A.M. Orville, and J.D. Lipscomb, Protocatechuate 3,4-Dioxygenase from Brevibacterium fuscum. D.M. Arciero, A.M. Orville, and J.D. Lipscomb, Protocatechuate 4,5-Dioxygenase from Pseudomonas testosteroni. S.A. Wolgel, and J.D. Lipscomb, Protocatechuate 2,3-Dioxygenase from Bacillus macerans. M.R. Harp


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© 1990
Academic Press
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