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Hybridization with Nucleic Acid Probes, Part II - 1st Edition - ISBN: 9780444898869, 9780080858937

Hybridization with Nucleic Acid Probes, Part II, Volume 24

1st Edition

Part II. Probe Labeling and Hybridization Techniques

Author: P. Tijssen
eBook ISBN: 9780080858937
Paperback ISBN: 9780444898852
Hardcover ISBN: 9780444898869
Imprint: Elsevier Science
Published Date: 14th October 1993
Page Count: 344
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Table of Contents

Excluding sub-sections. Full contents list available on request.

Chapters 1-6 are Part I of this title.

Chapter 7. Labeling of probes and their detection. 7.1. Choice of label and labeling method. 7.2. Radioisotopic labeling and detection of nucleic acids. 7.3. Nonradioactive primary labels. 7.4. Recognition systems. 7.5. Overview of enzymatic incorporation of labels into probes. 7.6. Uniform incorporation of labels in nucleic acids. 7.7. End-labeling of probes. 7.8. Chemical modification of probes to introduce labels. 7.9 Universal probes. 7.10. Biological probes. 8. Mixed-phase and solution hybridization formats. 8.1 Materials and solutions. 8.2. Slot/dot blot hybridization on membranes. 8.3. (Semi)-solution hybridization. 9. Hybridization after electrophoretic fractionation of nucleic acids. 9.1. Electrophoretic procedures. 9.2. Transfer procedures. 9.3. Hybridization in the gel or before electrophoresis. 10. Colony and plaque lift hybridization. 10.1. Colony and plaque hybridization: different approaches. 10.2. Colony hybridization. 10.3. Plaque hybridization. 10.4. Hybridization to colony or plaque nucleic acids. 10.5. Selection, packing and purification of clones. 11. In situ hybridization. 11.1. Theoretical considerations. 11.2. Cytological procedures. 11.3. Hybridization procedures. 11.4. Emerging in situ hybridization techniques. 12. Selected applications of hybridization. 12.1. Aims of subtractive or suppression hybridization. 12.2. Hybrid selection of mRNA. 12.3. S1 analysis of mRNA with DNA probes. 12.4. RNase protection assays. 12.5. Triple helices. 12.6. Current developments and prospects. References and Index (Parts I and II).


Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful supplement to other diagnostic tools.
These diagnostic methods are set out in a logical and clear two-part volume in this now famous Laboratory Techniques series. The volume is divided into theory and preparation (Part I), and probe labelling and hybridization techniques (Part II). Both parts are worthy additions to this series, designed for easy access of information on the laboratory bench.


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© Elsevier Science 1993
14th October 1993
Elsevier Science
eBook ISBN:
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About the Author

P. Tijssen

P. Tijssen

Affiliations and Expertise

Université de Quebec, Institut Armand Frappier, Villle de Laval, Quebec, Canada