Human Molecular Genetics - 1st Edition - ISBN: 9780120443109, 9780080536415

Human Molecular Genetics, Volume 8

1st Edition

Series Editors: Kenneth Adolph
eBook ISBN: 9780080536415
Imprint: Academic Press
Published Date: 26th April 1996
Page Count: 500
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Table of Contents

Mutation Detection in Human Genes. Gene Mapping, Cloning, Sequencing. Transcription: Promoters, Transcription Factors, Mrna. RNA Editing, Ribozymes, Antisense RNA. Genome Recombination Amplification. Receptors, Signal Transduction. The Mouse as a Model System for Human Molecular Genetics.


Description

Human Molecular Genetics is a practical guide to the applications of molecular biology and genetics techniques to human cells. A wide range of experimental procedures for investigating human genes and genomes are presented.

Key Features

  • Mutation Detection in Human Genes - chemical mismatch cleavage, DNA mini-sequencing, SSCP method, RT-PCR, electrophoretic mobility shift assay (EMSA), protein truncation test, chromosome deletion analysis.
  • Gene Mapping, Cloning, Sequencing - gene linkage determination, large-capacity cloning system, cDNA isolation, differential display method, primer-based DNA sequencing.
  • Transcription: Promoters, Transcription Factors, mRNA, - promotor mutation analysis, transcription factor identification, mRNA-protein interaction characterization.
  • RNA Editing, Ribozymes, Antisense RNA-mammalian RNA editing assays, ribozymes as genetic tools, antisense RNA technology.
  • Genome Recombination, Amplification - recombination assays for mammalian cells, gene amplification measurement.
  • Receptors, Signal Transduction - intra-cellular receptor characterization, analysis of signal transduction genes.
  • The Mouse as a Model System for Human Molecular Genetics - mouse genome methods (mouse crosses, somatic cell hybrids, YACs), mouse model for cardiovascular disease.

Details

No. of pages:
500
Language:
English
Copyright:
© Academic Press 1996
Published:
Imprint:
Academic Press
eBook ISBN:
9780080536415

About the Series Editors

Kenneth Adolph Series Editor

Affiliations and Expertise

University of Minnesota Medical School, Minneapolis, Minnesota, USA