COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Hormone Assay - 1st Edition - ISBN: 9781483231938, 9781483272863

Hormone Assay

1st Edition

Editor: C. W. Emmens
eBook ISBN: 9781483272863
Imprint: Academic Press
Published Date: 1st January 1950
Page Count: 568
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.


Hormone Assay considers assay methods in detail, focusing on vertebrate hormones. Statistics as applied to biological assay is another field in which recent advances have been phenomenal, although the application of modern methods to the design and analysis of many assays awaits full development. This simultaneous progress in the two fields of work has nevertheless provided a fruitful series of examples of how assays can be conducted in order to gain the greatest amount of information from the material available, and to express results in precise form. The book opens with a chapter on the use of and need for statistics in assays, followed by a chapter on insulin, which exemplifies some of the methods described. Subsequent chapters discuss assay methods for parathyroid hormone, adrenalin, pituitary lobe hormones, melanophore expanding hormone from the pituitary, gonadotropins, adrenocorticotropin, thyrotropic hormone, and lactogenic hormone. Also covered are assay procedures for mammogenic hormone, mammogenic hormone, androgens, and estrogens.

Table of Contents

List Of Contributors


I. Statistical Methods

I. Introduction

II. Statistical Terminology and Procedure

III. Types of Response

IV. Assays Based on Quantitative Responses

V. Assays Based on Qualitative Responses

VI. Predicting Assay Requirements


II. Insulin

I. Introduction

II. The Standard Preparation

III. The Rabbit Method of Assay

IV. The Mouse Method of Assay

V. Comparison of Rabbit and Mouse Methods of Assay

VI. The Assay of Insulin in Blood


III. Parathyroid Hormone

I. Introduction

II. Possibilities of a Standard Preparation

III. The Unit of Parathyroid Activity

IV. Methods of Assay


IV. Biological and Chemical Assay of Adrenalin

I. Introduction

II. The Blood Pressure of a Cat or Dog

III. The Rabbit's Intestine

IV. Shaw's Chemical Method and Specific Test

V. The Straub Frog Heart

VI. The Perfused Frog Heart

VII. The Perfused Frog Blood Vessels

VIII. The Hen's Rectal Caecum

IX. The Perfused Rabbit's Ear

X. The Isolated Rat's Uterus

XI. The Fluorescent Reaction

XII. Other Chemical Methods

XIII. Noradrenalin


V. Posterior Pituitary Lobe Hormones

I. Introduction

II. Standard Preparation

III. Methods of Assay


VI. Biological Assay of the Melanophore Expanding Hormone from the Pituitary

I. Introduction

II. Brief Historical Note

III. Theoretical Considerations

IV. Practical Details

V. Performance of an Assay

VI. Sample Assays

VII. Accuracy to be Expected

VIII. Implications of Disproportionate 'B'/Pressor and 'B '/Oxytocic Ratios

IX. Assay of Material Subjected to Caustic Soda Treatment

X. Assay of 'B' in Urine, Blood, and Tissue Extracts

XI. The Question of Whether the Pars Intermedia Manufactures Two Separate Chromatophore Excitants

XII. Summary



VII. Gonadotropins

I. Historical Introduction

II. Site of Production and Classification of Gonadotropins

III. General Principles for the Assay of Gonadotropins

IV. Assay of Chorionic Gonadotropin

V. Assay of Pregnant Mares' Serum Gonadotropin

VI. Assay of Hypophyseal Gonadotropins

VII. Summary and Conclusions


VIII. Adrenocorticotropin

I. The Bioassay of ACTH in the Intact Animal

II. The Bioassay of ACTH in the Hypophysectomized Animal


IX. Thyrotropic Hormone

I. Introduction

II. Factors Influencing the Sensitivity of the Thyroid Gland

III. Methods of Assay of Thyrotropic Hormone



X. Lactogenic Hormone

I. Introduction

II. Mammalian Assay Methods

III. Pigeon Assay Methods

IV. Comparison of Assay Methods Using International Standard Lactogenic Hormone

V. Comparison of Lactogenic Hormone Content in Pituitaries of Various Mature Animals

VI. Comparison of Lactogenic Hormone Content in Pituitaries of Various Animals as Affected by Pregnancy and Lactation, Suckling, and Following Estrogen Stimulation


XI. Mammogenic Hormone

I. Introduction

II. Preparation of Whole Mounts of Mammary Glands

III. Assay of Duct Growth

IV. Assay of Lobule-Alveolar Growth

V. Summary


XII. Growth Hormone

I. Introduction

II. Well-Established Procedures for the Bioassay of Growth Hormone

III. Suggested Methods for t he Bioassay of Growth Hormone

IV. Comment on Methods


XIII. Androgens

I. Introduction

II. Surgical Procedures

III. Bird Methods

IV. Mammalian Assays

V. Summary of Methods


XIV. Adrenal Cortical Hormones

I. Introduction

II. Adrenalectomy

III. Survival-Growth Methods

IV. Electrolyte Methods

V. Carbohydrate Methods

VI. Stress Tests

VII. Summary


XV. The Chemical Assay of Steroids of the Androgen and Adrenocortical Hormone Groups

I. Introduction—Chemical Structure of the Androgen and Adrenocortical Hormone Groups in Relation to Assay Methods

II. The Androgen Group

III. The Adrenocortical Hormone Group—The Development of Extraction and Chemical Assay Methods


XVI. Estrogens

I. Introduction

II. Types of Estrogen

III. Assays Based on Vaginal Cornification (Allen-Doisy Tests)

IV. Modifications of the Allen-Doisy Test

V. Interpretation of Results with Allen-Doisy Tests

VI. Intravaginal Allen-Doisy Tests

VII. Assays Based on Uterine Weight

VIII. Assays Based on Vaginal Opening


XVII. Hormones of the Corpus Luteum

I. Progestogens

II. Relaxin


XVIII. Chemical Assay of Estrogens and Pregnanediol

I. Introduction

II. Estrogens

III. Pregnanediol


XIX. Thyroidal Substances

I. Introduction

II. Thyroid Standards and Routes of Administration

III. Assays Based on Elevation of the Metabolic Rate

IV. Assays Based on Loss in Body Weight

V. Growth Restoration in Thyroidectomized Animals

VI. Maintenance of Thyroid-Pituitary Balance in Thiouracil-Treated Animals

VII. Assays Based on Stimulation of Metamorphosis in Amphibian Tadpoles


XX. The Chemical Assay of Thyroxine and Other Substances with Thyroidal Activity

I. Introduction

II. Methods of Chemical Assay of Compounds Having Thyroidal Activity

III. The Determination of Iodine in Organic Combination

IV. Discussion




No. of pages:
© Academic Press 1950
1st January 1950
Academic Press
eBook ISBN:

About the Editor

C. W. Emmens

Ratings and Reviews