Preparation of Chemical Probes: M.C. Beinfeld, Strategy and Methodology for the Development of Antisera against Procholecystokinin. J.P. Tam, A High-Density Multiple Antigen Peptide System for the Preparation of Antipeptide Antibodies. K.L. Bost and J.E. Blalock, Preparation and Use of Complementary Peptides. J. Schwartz and W. Vale, Fluorescent and Cytotoxic Analogs of CRF Probes for Studying Target Cells in Heterogeneous Populations. Equipment and Technology: C. Denef, Methods to Study Cell-to-Cell Communication in Peptide Target Cells of the Anterior Pituitary. P.C. Andrews and J.E. Dixon, Application of Fast Atom Bombardment Mass Spectrometry to Posttranslational Modifications of Neuropeptides. E.D. French and J.T. Williams, Electrophysiological Analysis of Opioid Peptides: Extracellular and Intracellular Approaches. M.I. Phillips and R.A. Palovcik, Dose Response Testing of Peptides by Hippocampal Brain Slice Recording. T.L. Croxton, W.McD. Armstrong, and N. Ben-Jonathan, Patch Clamp Recording from Anterior Pituitary Cells Identified by a Reverse Hemolytic Plaque Assay. J.E. Levine and K.D. Powell, Microdialysis for Measurement of Neuroendocrine Peptides. K.M. Kendrick, Use of Microdialysis in Neuroendocrinology. D.D. Rasmussen, In Vitro Perifusion of Human Hypothalamic and Pituitary Tissue. I. Winicov and M.C. Gershengorn, Transient Permeabilization of Endocrine Cells: A New Approach to the Study of Inositol Lipid Metabolism. T.F.J. Martin, Cell-Cracking: Permeabilizing Cells to Macromolecular Probes. I. Murai, W.C. Low, and N. Ben-Jonathan, Microsurgical Techniques for Studying Functional Correlates of Hypothalamohypophysal Axis. G. Martinez de la Escalera, K.C. Swearingen, and R.I. Weiner, Superfusion and Static Culture Techniques for Measurement of Rapid Changes in Prolactin Secretion. D.A. Leong, Direct Observation of Intracellular Calcium Levels in Single Rat Anterior Pituitary Cells. Preparation and Maintenance of Biological Materials: C.A. McArdle and P.M. Conn, The Use of Protein Kinase C-Depleted Cells for Investigation of the Role of Protein Kinase C in Stimulus-Response Coupling in the Pituitary. J. Eng and R.S. Yalow, Purification of Neuropeptides: CCK8 and VIP. C.D. Scott and R.C. Baxter, Purification and Characterization of Insulinlike Growth Factor II Receptors. Quantitation of Neuroendocrine Substances: M.E. Kendall and W.C. Hymer, Measurement of Hormone Secretion from Individual Cells by the Cell Blot Assay. M.M. Chien and J.C. Cambier, Measurement of Receptor Coupling to Phosphoinositide Hydrolysis across Isolated Cell Membranes. M.S. Shearman, K. Ogita, U. Kikkawa and Y. Nishizuka, Rapid Method for the Resolution of Protein Kinase C Subspecies from Rat Brain Tissue. T. Brock, J. Humm, and J.S. Kizer, Assay of Peptidylglycine Monooxygenase: Glycine-Directed Amidating Enzyme. J.B. Koger, J. Humm, and J.S. Kizer, Assay of Glutaminylpeptide Cyclase. C.H. Emerson, Primary Thyrotropin-Releasing Hormone-Degrading Enzymes. J.C. Porter, P.S. Wang, W. Kedzierski, and H.A. Gonzalez, Quantification of the Mass of Tyrosine Monooxygenase in the Median Eminence and Superior Cervical Ganglion. J.P.H. Burbach and B. Liu, Measurement of Vasopressin-Converting Aminopeptidase Activity and Vasopressin Metabolites. H.H.M. Van Tol and J.P.H. Burbach, Quantitation of Vasopressin and Oxytocin mRNA Levels in the Brain. K.D. Dahn, X.-C. Jia, and A.J.W. Hsueh, Granulosa Cell Aromatase Bioassay for Follicle-Stimulating Hormone. T.A. Bicsak, C.M. Hekman, and A.J.W. Hsueh, Neuroendocrine Regulation of Oocyte Tissue Plasminogen Activator. M.R. Brown, R. Allen, and L.A. Fisher, Assessment of Peptide Regulation of the Autonomic Nervous System. L.H. Lazarus and W.E. Wilson, Recognition, Purification, and Structural Elucidation of Mammalian Physalaemin-Related Molecules. M. Goedert, Radioligand Binding Assays for the Study of Neurotensin Receptors. T.W. Moody, R.M. Kris, G. Fiskum, C.D. Linden, M. Berg, and J. Schlessinger, Characterization of Receptors for Bombesin/GRP in Human and Murine Cells. E. Hazum, Isolation and Identification of Neuroendocrine Peptides from Milk. M.D. Culler and A. Negro-Vilar, Passive Immunoneutralization: Regulation of Basal and Pulsatile Hormone Secretion. W. Wetsel and A. Negro-Vilar, Combined Antibody-HPLC Approach to Assess Prohormone Processing. K. Hermann, M.K. Raizada, M.I. Phillips, Chromatographic Methods for the Characterization of Angiotensin in Brain Tissue. C.H. Emerson, Measurement of Thyrotropin-Releasing Hormone and Its Metabolites. G. Valiquette and S. Neubort, Monoclonal Antibodies: Uses in Studies on Vasopressin. J.M. Vaughan, J. Rivier, A.Z. Corrigan, R. McClintock, C.A. Campen, D. Jolley, J.K. Vogelmayr, C.W. Bardin, C. Rivier, and W. Vale, Detection and Purification of Inhibin Using Antisera Generated against Synthetic Peptide Fragments. M. Blum, Regulation of Neuroendocrine Peptide Gene Expression. J.E. Krause, J.D. Cremins, M.S. Carter, E.R. Brown, and M.R. MacDonald, Solution Hybridization-Nuclease Protection Assays for the Sensitive Detection of Differentially Spliced Substance P- and Neurokinin A-Encoding Messenger RNAs. W.A. Bank and A.J. Kastin, Quantifying Carrier-Mediated Transport of Peptides from the Brain to the Blood. J.R. Reeve, Jr. and J.H. Walsh, Characterizing Molecular Heterogeneity of Gastrin-Releasing Peptide and Related Peptides. Use of Chemical Probes. J.D. White and E.F. LaGamma, Determination of Neuropeptide Gene Transcription in Central and Peripheral Nervous System Tissue by Nuclear Run-on Assay. D.W. Crabb, C.D. Minth, and J.E. Dixon, Assaying the Reporter Gene Chloramphenicol Acetyltransferase. S.W. Young, III, In Situ Hybridization Histochemical Detection of Neuropeptide mRNAs Using DNA and RNA Probes. A. Barnea, Use of Metal Complexes in Neuroendocrine Studies. M.C. Aguila and S.M. McCann, Methods for the Study of Somatostatin. E. Hazum, Mapping of the GnRH-Receptor Binding Site Using Selective Chemical Modifications. Localization of Neuroendocrine Substances: G.R. Uhl, In Situ Hybridization: Issues with Quantitation Using Radiolabeled Hybridization Probes. P.C. Emson, H. Arai, S. Agrawal, C. Christodoulou, and M.J. Gait, Nonradioactive Methods of in Situ Hybridization-Visualization of Neuroendocrine mRNA. F. Baldino, Jr., M.-F. Chesselet, and M.E. Lewis, High Resolution in Situ Hybridization Histochemistry. J.S. Schwaber, In Situ Hybridization Histochemistry Combined with Markers of Neuronal Connectivity. A.E. Bishop and J.M. Polak, Cytochemical Techniques for Studying Diffuse Neuroendocrine System. M.E. Lewis, W.T. Rogers, R.G. Krause II, and J.S. Schwaber, Quantitation and Digital Representation of in Situ Hybridization Histochemistry. J.T. McCabe, R.A. Desharnais, and D.W. Pfaff, Graphical and Statistical Approaches to Data Analysis for in Situ Hybridization. Author Index. Subject Index.
Volume 168 of Methods in Enzymology will prove invaluable not only to those in the field but also to those in related disciplines who find their studies becoming closely linked to the neurosciences. Methodologies are presented to allow easy adaptation to new systems and to stress their general applicability and potential limitations.
- No. of pages:
- © Academic Press 1989
- 28th March 1989
- Academic Press
- eBook ISBN:
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P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.
Texas Tech University Health Sciences Center, Lubbock, USA
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA
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