Hormone Action, Part K: Neuroendocrine Peptides, Volume 168

1st Edition

Print ISBN: 9780121820695
eBook ISBN: 9780080882857
Imprint: Academic Press
Published Date: 28th March 1989
Page Count: 906


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Volume 168 of Methods in Enzymology will prove invaluable not only to those in the field but also to those in related disciplines who find their studies becoming closely linked to the neurosciences. Methodologies are presented to allow easy adaptation to new systems and to stress their general applicability and potential limitations.

Table of Contents

Preparation of Chemical Probes:

M.C. Beinfeld, Strategy and Methodology for the Development of Antisera against Procholecystokinin.

J.P. Tam, A High-Density Multiple Antigen Peptide System for the Preparation of Antipeptide Antibodies.

K.L. Bost and J.E. Blalock, Preparation and Use of Complementary Peptides.

J. Schwartz and W. Vale, Fluorescent and Cytotoxic Analogs of CRF Probes for Studying Target Cells in Heterogeneous Populations.

Equipment and Technology:

C. Denef, Methods to Study Cell-to-Cell Communication in Peptide Target Cells of the Anterior Pituitary.

P.C. Andrews and J.E. Dixon, Application of Fast Atom Bombardment Mass Spectrometry to Posttranslational Modifications of Neuropeptides.

E.D. French and J.T. Williams, Electrophysiological Analysis of Opioid Peptides: Extracellular and Intracellular Approaches.

M.I. Phillips and R.A. Palovcik, Dose Response Testing of Peptides by Hippocampal Brain Slice Recording.

T.L. Croxton, W.McD. Armstrong, and N. Ben-Jonathan, Patch Clamp Recording from Anterior Pituitary Cells Identified by a Reverse Hemolytic Plaque Assay.

J.E. Levine and K.D. Powell, Microdialysis for Measurement of Neuroendocrine Peptides.

K.M. Kendrick, Use of Microdialysis in Neuroendocrinology.

D.D. Rasmussen, In Vitro Perifusion of Human Hypothalamic and Pituitary Tissue.

I. Winicov and M.C. Gershengorn, Transient Permeabilization of Endocrine Cells: A New Approach to the Study of Inositol Lipid Metabolism.

T.F.J. Martin, Cell-Cracking: Permeabilizing Cells to Macromolecular Probes.

I. Murai, W.C. Low, and N. Ben-Jonathan, Microsurgical Techniques for Studying Functional C


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© Academic Press 1989
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