Preparation of Chemical Probes:
M.C. Beinfeld, Strategy and Methodology for the Development of Antisera against Procholecystokinin.
J.P. Tam, A High-Density Multiple Antigen Peptide System for the Preparation of Antipeptide Antibodies.
K.L. Bost and J.E. Blalock, Preparation and Use of Complementary Peptides.
J. Schwartz and W. Vale, Fluorescent and Cytotoxic Analogs of CRF Probes for Studying Target Cells in Heterogeneous Populations.
Equipment and Technology:
C. Denef, Methods to Study Cell-to-Cell Communication in Peptide Target Cells of the Anterior Pituitary.
P.C. Andrews and J.E. Dixon, Application of Fast Atom Bombardment Mass Spectrometry to Posttranslational Modifications of Neuropeptides.
E.D. French and J.T. Williams, Electrophysiological Analysis of Opioid Peptides: Extracellular and Intracellular Approaches.
M.I. Phillips and R.A. Palovcik, Dose Response Testing of Peptides by Hippocampal Brain Slice Recording.
T.L. Croxton, W.McD. Armstrong, and N. Ben-Jonathan, Patch Clamp Recording from Anterior Pituitary Cells Identified by a Reverse Hemolytic Plaque Assay.
J.E. Levine and K.D. Powell, Microdialysis for Measurement of Neuroendocrine Peptides.
K.M. Kendrick, Use of Microdialysis in Neuroendocrinology.
D.D. Rasmussen, In Vitro Perifusion of Human Hypothalamic and Pituitary Tissue.
I. Winicov and M.C. Gershengorn, Transient Permeabilization of Endocrine Cells: A New Approach to the Study of Inositol Lipid Metabolism.
T.F.J. Martin, Cell-Cracking: Permeabilizing Cells to Macromolecular Probes.
I. Murai, W.C. Low, and N. Ben-Jonathan, Microsurgical Techniques for Studying Functional C
Volume 168 of Methods in Enzymology will prove invaluable not only to those in the field but also to those in related disciplines who find their studies becoming closely linked to the neurosciences. Methodologies are presented to allow easy adaptation to new systems and to stress their general applicability and potential limitations.
- No. of pages:
- © Academic Press 1989
- 28th March 1989
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Volume @qu:"Neuroendocrinologists, neuroscientists, and reproductive physiologists should peruse this book for new ideas/approaches to their current research problems." @source:--ADOLESCENT AND PEDIATRIC GYNECOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve
Texas Tech University Health Sciences Center, Lubbock, USA
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA