Volume 168 of Methods in Enzymology will prove invaluable not only to those in the field but also to those in related disciplines who find their studies becoming closely linked to the neurosciences. Methodologies are presented to allow easy adaptation to new systems and to stress their general applicability and potential limitations.
Preparation of Chemical Probes:
M.C. Beinfeld, Strategy and Methodology for the Development of Antisera against Procholecystokinin.
J.P. Tam, A High-Density Multiple Antigen Peptide System for the Preparation of Antipeptide Antibodies.
K.L. Bost and J.E. Blalock, Preparation and Use of Complementary Peptides.
J. Schwartz and W. Vale, Fluorescent and Cytotoxic Analogs of CRF Probes for Studying Target Cells in Heterogeneous Populations.
Equipment and Technology:
C. Denef, Methods to Study Cell-to-Cell Communication in Peptide Target Cells of the Anterior Pituitary.
P.C. Andrews and J.E. Dixon, Application of Fast Atom Bombardment Mass Spectrometry to Posttranslational Modifications of Neuropeptides.
E.D. French and J.T. Williams, Electrophysiological Analysis of Opioid Peptides: Extracellular and Intracellular Approaches.
M.I. Phillips and R.A. Palovcik, Dose Response Testing of Peptides by Hippocampal Brain Slice Recording.
T.L. Croxton, W.McD. Armstrong, and N. Ben-Jonathan, Patch Clamp Recording from Anterior Pituitary Cells Identified by a Reverse Hemolytic Plaque Assay.
J.E. Levine and K.D. Powell, Microdialysis for Measurement of Neuroendocrine Peptides.
K.M. Kendrick, Use of Microdialysis in Neuroendocrinology.
D.D. Rasmussen, In Vitro Perifusion of Human Hypothalamic and Pituitary Tissue.
I. Winicov and M.C. Gershengorn, Transient Permeabilization of Endocrine Cells: A New Approach to the Study of Inositol Lipid Metabolism.
T.F.J. Martin, Cell-Cracking: Permeabilizing Cells to Macromolecular Probes.
I. Murai, W.C. Low, and N. Ben-Jonathan, Microsurgical Techniques for Studying Functional C
- No. of pages:
- © Academic Press 1989
- 28th March 1989
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Volume @qu:"Neuroendocrinologists, neuroscientists, and reproductive physiologists should peruse this book for new ideas/approaches to their current research problems." @source:--ADOLESCENT AND PEDIATRIC GYNECOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY