Histones and Other Nuclear Proteins

Histones and Other Nuclear Proteins

1st Edition - January 1, 1965

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  • Author: Harris Brusch
  • eBook ISBN: 9780323157926

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Histones and Other Nuclear Proteins provides an introduction to the status of research and knowledge of histones and nuclear proteins. This book discusses the classification of the nuclear proteins, the role of proteins in control of the genome, repressor substances, and history of the nuclear proteins. Other topics include the primary structure of protamines, amino acid composition of histones, and direct isolation of nucleoproteins. The relationship of histones to chromosome structure, effects of histones on DNA synthesis, and sequential analysis of amino acids in the N-proline histone fraction is also deliberated. This text likewise covers the labeling of histones in vivo, physical and chemical properties of the acidic nuclear proteins, and enzymes involved in synthesis of nucleic acids. This publication is valuable to chemists and students aiming to acquire knowledge of histones and other nuclear proteins.

Table of Contents

  • Preface



    Classification of the Nuclear Proteins

    The Role of Proteins in Control of the Genome

    Repressor Substances

    History of the Nuclear Proteins

    The Histones

    The Acidic Nuclear Proteins

    Enzymes of the Nucleus


    Part I The Basic Nuclear Proteins

    Chapter I. The Protamines

    General Discussion

    Primary Structure of Protamines

    Functions of the Protamines


    Chapter II. Types, Composition, and Number of Histones

    Classification of the Histones

    Amino Acid Composition of Histones

    Amino Acid Composition of Products of Recent Fractionation Procedures

    NH2-Terminal Amino Acid Analysis of Histones

    Physical Methods for Classification of the Histones

    Sedimentation Analysis of the Histones

    The Number of Histones

    Distribution of Histones in Nature


    Chapter III. Isolation of the Histones


    Isolation of Nuclei

    Direct Isolation of Nucleoproteins

    Fractionation of the Histones

    Older Methods for Fractionation of the Histones

    Chromatography of the Histones

    Electrophoresis of Histones

    Sedimentation of the Histones


    Chapter IV. Spatial Relationships between DNA and Histones


    Relationship of Histones to Chromosome Structure

    Models of the Structure of the Chromosome

    Special Linkages of Histones and DNA


    Chapter V. Functions of the Histones


    The Concept That Histones are Gene Modifiers

    Suppression of Biosynthesis of RNA

    Effects of Histones on DNA Synthesis

    Numerical Relationships between the Suppressive Potentialities of Histones and Loci of DNA

    Histones of Specialized Tissues

    Histones of Cancer Cells

    Histones as Ribonucleases

    Other Potential Functions of the Histones


    Chapter VI. The Primary Structure of Histones


    Early Studies on Peptides of the Histones

    Peptides of the N-Proline Histone Fraction

    Elementary Amino Acid Analysis of Peptides of the N-Proline Histone Fraction

    Sequential Analysis of Amino Acids in the N-Proline Histone Fraction

    The 2a or N-Acetylalanine Histone Fraction


    Chapter VII. Metabolism of Histones


    Synthesis of Histones in Dividing Cells

    Labeling of Histones in Vivo

    Labeling of Specific Histones in Vivo

    Fraction of Total Histone Turning Over in One Day

    When Are Histones Synthesized in the Cell Cycle?

    Catabolism of Histones


    Part II The Acidic Nuclear Proteins and the Nuclear Enzymes

    Chapter VIII. The Acidic Nuclear Proteins


    Quantitative Studies on Acidic Nuclear Proteins

    Nuclear Ribonucleoproteins

    Physical and Chemical Properties of the Acidic Nuclear Proteins

    Subfractionation of the Acidic Nuclear Proteins

    Amino Acid Analysis of the Acidic Nuclear Proteins

    NH2-Terminal Amino Acids of the Acidic Nuclear Proteins

    Biosynthesis of the Acidic Nuclear Proteins

    Effects of Antitumor Agents on the Biosynthesis of Acidic Nuclear Proteins


    Chapter IX. Nuclear Enzymes


    DPN Synthetase or Pyrophosphorylase

    Enzymes Involved in Synthesis of Nucleic Acids

    RNA Polymerase

    Nuclear Ribonucleases

    Oxidative Enzymes in the Nucleus

    Enzymes of Protein Synthesis

    Hydrolytic Enzymes of Nuclei

    Other Nuclear Enzymes


    Author Index

    Subject Index

Product details

  • No. of pages: 280
  • Language: English
  • Copyright: © Academic Press 1965
  • Published: January 1, 1965
  • Imprint: Academic Press
  • eBook ISBN: 9780323157926

About the Author

Harris Brusch

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