Histochemical Techniques

Histochemical Techniques

2nd Edition - October 6, 1975

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  • Author: J. D. Bancroft
  • eBook ISBN: 9781483164717

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Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.

Table of Contents

  • Foreword


    1 Introduction

    2 Fixation

    3 Paraffin Sections

    4 Frozen Sections

    5 Cryostats

    6 Freeze Drying and Freeze Substitution

    7 Carbohydrates

    8 Proteins and Amino Acids

    9 Amyloid

    10 Lipids

    11 Nucleic Acids

    12 Pigments

    13 Minerals

    14 Principles of Enzyme Histochemistry

    15 Phosphatases

    16 Esterases

    17 Oxidoreductases I. Oxidases

    18 Oxidoreductases II. Dehydrogenases

    19 Miscellaneous Techniques

    20 Ultra-histochemistry


    I List of Methods

    II Preparation of Dyes and Mounting Media

    III Diazonium Salts

    IV Buffer Tables and Preparation of Molar and Normal Solutions

    V Some Sources of Supply for the Chemicals and Reagents Used in Methods Given in this Book


Product details

  • No. of pages: 358
  • Language: English
  • Copyright: © Butterworth-Heinemann 1975
  • Published: October 6, 1975
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483164717

About the Author

J. D. Bancroft

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