High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications

High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications

1st Edition - July 18, 1996
This is the Latest Edition
  • Editors: Barry Karger, William Hancock
  • Hardcover ISBN: 9780121821722

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

  • Liquid chromatography
  • Electrophoresis
  • Mass spectrometry


Biochemists, molecular biologists, biotechnologists, biophysicists, and pharmaceutical chemists.

Table of Contents

  • Liquid Chromatography: C.T. Mant and R.S. Hodges, Analysis of Peptides by High-Performance Liquid Chromatography. E.R. Hoff and R.C. Chloupek, Analytical Peptide Mapping of Recombinant DNA-Derived Proteins byReversed-Phase High-Performance Liquid Chromatography. K.M. Swiderek, T.D. Lee, and J.E. Shively, Trace Structural Analysis of Proteins. L.J. Janis, P.M. Kovach, R.M. Riggin, and J.K. Towns, Protein Liquid Chromatographic Analysis in Biotechnology. D. Josic and K. Zeilinger, Membrane Proteins. R.R. Townsend, L.J. Basa, and M.W. Spellman, Identification and Characterization of Glycopeptides in Tryptic Maps by High-pH Anion-Exchange Chromatography. J.R. Thayer, R.M. McCormick, and N. Avdalovic, High Resolution Nucleic Acid Separations by High-Performance Liquid Chromatography. Electrophoresis: Slab Gel Electrophoresis: High Resolution: M.J. Dunn and J.M. Corbett, Two-Dimensional Polyacrylamide Gel Electrophoresis. K. Shimura and K.-i. Kasai, Affinophoresis: Selective Electrophoretic Separation of Proteins Using Specific Carriers. L.M. Smith, R.L. Brumley, Jr., E.C. Buxton, M. Giddings, M. Marchbanks, and X. Tong, High-Speed Automated DNA Sequencing in Ultrathin Slab Gels. Capillary Electrophoresis: E.C. Rickard and J.K. Towns, Applications of Capillary Zone Electrophoresis to Peptide Mapping. G. Teshima and S.-L. Wu, Capillary Electrophoresis Analysis of Recombinant Proteins. B.L. Karger, F. Foret, and J. Berka, Applications of Polymer Matrices to DNA and Protein Analysis by Capillary Electrophoresis. M.V. Novotny, Glycoconjugate Analysis by Capillary Electrophoresis. Mass Spectrometry: J.R. Yates, Protein Structure Analysis by Mass Spectrometry. V.N. Reinhold, B.B. Reinhold, and S. Chan, Carbohydrate Sequence Analysis by Electrospray Ionization–Mass Spectrometry. W.S. Hancock, A. Apffel, J. Chakel, C. Souders, T.M. Timkulu, E. Pungor,Jr., and A.W. Guzzetta, Reversed-Phase Peptide Mapping of Recombinant Human Glycoproteins Using Liquid Chromatography/Electrospray Ionization–Mass Spectrometry. B.L. Gillece-Castro and J.T. Stults, Peptide Characterization by Mass Spectrometry. R.D. Smith, H.R. Udseth, J.H. Wahl, D.R. Goodlett, and S.A. Hofstadler, Capillary Electrophoresis–Mass Spectrometry. J. Frenz, Trace Analysis Peptide Mapping by High-Performance Displacement ChromatographyR.W. Giese, M. Saha, S. Abdel-Baky, and K. Allam, Measuring DNA Adducts by Gas Chromatography–Electron Capture–Mass Spectrometry: Trace Organic Analysis. Author Index. Subject Index.

Product details

  • No. of pages: 555
  • Language: English
  • Copyright: © Academic Press 1996
  • Published: July 18, 1996
  • Imprint: Academic Press
  • Hardcover ISBN: 9780121821722
  • About the Serial Volume Editors

    Barry Karger

    Affiliations and Expertise

    Barnett Institute, Northeastern University, Boston, Massachusetts, U.S.A.

    William Hancock

    Affiliations and Expertise

    Hewlett-Packard, Palo Alto, California, U.S.A.

    About the Editors in Chief

    John Abelson

    Affiliations and Expertise

    California Institute of Technology, Division of Biology, Pasadena, U.S.A.

    Melvin Simon

    Affiliations and Expertise

    The Salk Institute, La Jolla, CA, USA