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High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications - 1st Edition - ISBN: 9780121821722, 9780080883847

High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications, Volume 271

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Barry Karger William Hancock
Hardcover ISBN: 9780121821722
eBook ISBN: 9780080883847
Imprint: Academic Press
Published Date: 18th July 1996
Page Count: 555
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Table of Contents

Liquid Chromatography: C.T. Mant and R.S. Hodges, Analysis of Peptides by High-Performance Liquid Chromatography. E.R. Hoff and R.C. Chloupek, Analytical Peptide Mapping of Recombinant DNA-Derived Proteins byReversed-Phase High-Performance Liquid Chromatography. K.M. Swiderek, T.D. Lee, and J.E. Shively, Trace Structural Analysis of Proteins. L.J. Janis, P.M. Kovach, R.M. Riggin, and J.K. Towns, Protein Liquid Chromatographic Analysis in Biotechnology. D. Josic and K. Zeilinger, Membrane Proteins. R.R. Townsend, L.J. Basa, and M.W. Spellman, Identification and Characterization of Glycopeptides in Tryptic Maps by High-pH Anion-Exchange Chromatography. J.R. Thayer, R.M. McCormick, and N. Avdalovic, High Resolution Nucleic Acid Separations by High-Performance Liquid Chromatography. Electrophoresis: Slab Gel Electrophoresis: High Resolution: M.J. Dunn and J.M. Corbett, Two-Dimensional Polyacrylamide Gel Electrophoresis. K. Shimura and K.-i. Kasai, Affinophoresis: Selective Electrophoretic Separation of Proteins Using Specific Carriers. L.M. Smith, R.L. Brumley, Jr., E.C. Buxton, M. Giddings, M. Marchbanks, and X. Tong, High-Speed Automated DNA Sequencing in Ultrathin Slab Gels. Capillary Electrophoresis: E.C. Rickard and J.K. Towns, Applications of Capillary Zone Electrophoresis to Peptide Mapping. G. Teshima and S.-L. Wu, Capillary Electrophoresis Analysis of Recombinant Proteins. B.L. Karger, F. Foret, and J. Berka, Applications of Polymer Matrices to DNA and Protein Analysis by Capillary Electrophoresis. M.V. Novotny, Glycoconjugate Analysis by Capillary Electrophoresis. Mass Spectrometry: J.R. Yates, Protein Structure Analysis by Mass Spectrometry. V.N. Reinhold, B.B. Reinhold, and S. Chan, Carbohydrate Sequence Analysis by Electrospray Ionization–Mass Spectrometry. W.S. Hancock, A. Apffel, J. Chakel, C. Souders, T.M. Timkulu, E. Pungor,Jr., and A.W. Guzzetta, Reversed-Phase Peptide Mapping of Recombinant Human Glycoproteins Using Liquid Chromatography/Electrospray Ionization–Mass Spectrometry. B.L. Gillece-Castro and J.T. Stults, Peptide Characterization by Mass Spectrometry. R.D. Smith, H.R. Udseth, J.H. Wahl, D.R. Goodlett, and S.A. Hofstadler, Capillary Electrophoresis–Mass Spectrometry. J. Frenz, Trace Analysis Peptide Mapping by High-Performance Displacement ChromatographyR.W. Giese, M. Saha, S. Abdel-Baky, and K. Allam, Measuring DNA Adducts by Gas Chromatography–Electron Capture–Mass Spectrometry: Trace Organic Analysis. Author Index. Subject Index.


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

  • Liquid chromatography
  • Electrophoresis
  • Mass spectrometry


Biochemists, molecular biologists, biotechnologists, biophysicists, and pharmaceutical chemists.


No. of pages:
© Academic Press 1996
18th July 1996
Academic Press
Hardcover ISBN:
eBook ISBN:


Praise for the Volume
"These two books (volumes 270 and 271) comply with and add to the outstanding reputation of the Methods in Enzymology series as a primary source for detailed and practical information on techniques used in the biological sciences. The excellent, step-by-step description of the techniques described, the clear schematic illustrations, and the frequent addition of technical tricks which are not normally described in the methods sections of scientific journals will facilitate the successful application of the techniques... Together, Methods in Enzymology Volumes 270 and 271 are an invaluable resource for the beginner and the experienced researcher alike." --Daniel Figeys and Ruedi Aebersold in ANALYTICAL BIOCHEMISTRY

Praise for the Series
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

"Incomparably useful." --ANALYTICAL BIOCHEMISTRY

"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY

"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Ratings and Reviews

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Barry Karger

Affiliations and Expertise

Barnett Institute, Northeastern University, Boston, Massachusetts, U.S.A.

William Hancock

Affiliations and Expertise

Hewlett-Packard, Palo Alto, California, U.S.A.