High-Resolution Electrophoresis and Immunofixation

High-Resolution Electrophoresis and Immunofixation

Techniques and Interpretation

2nd Edition - September 21, 1994

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  • Author: David F. Keren
  • eBook ISBN: 9781483221090

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Description

High-Resolution Electrophoresis and Immunofixation: Techniques and Interpretation, Second Edition presents the important developments in the technology of serum protein electrophoresis. This book discusses the electrophoretic patterns that one encounters when using high-resolution electrophoresis and immunofixation. Organized into nine chapters, this edition begins with an overview of the migration of charged particles in an electrical field. This text then presents the study of nasal and aural fluids looking for cerebrospinal fluid (CSF)-specific transferrin to detect CSF leakage due to a skull fracture or tumor. Other chapters consider some detailed studies of strategies employing immunoglobulin quantification and high-resolution electrophoresis to detect monoclonal gammopathies. This book discusses as well the three available strategies to enhance the efficiency of diagnosis of monoclonal gammopathies. The final chapter deals with the principles of high-resolution electrophoresis and immunofixation electrophoresis (IFE). This book is a valuable resource for pathologists, technologists, epidemiologists, physicians, and clinicians.

Table of Contents


  • Preface to the First Edition

    Preface to the Second Edition

    1. Methods and Rationale for High-Resolution Electrophoresis

    Review of Protein Structure and Electrophoretic Techniques

    References

    2. Techniques for High-Resolution Electrophoresis

    Principles of High-Resolution Electrophoresis

    High-Resolution Electrophoresis on Agarose

    High-Resolution Electrophoresis on Cellulosic Media

    Densitometric Scanning of High-Resolution Electrophoresis

    High-Resolution Electrophoresis Versus Standard Electrophoresis

    Cost and High-Resolution Electrophoresis

    Capillary Electrophoresis

    Two-Dimensional Electrophoresis

    References

    3. Proteins in Serum Identified by High-Resolution Electrophoresis

    Proteins Identified by High-Resolution Electrophoresis

    Alpha Region

    Inter-Alpha-1, -Alpha-2 Region

    Beta Region

    Gamma Region

    References

    4. Approaches to Pattern Interpretation in Serum

    Initial Processing of the Sample

    Overview of the Electrophoretic Strip

    Interpretation of the Individual Patient's Sample

    References

    5. Approaches to Pattern Interpretation in Cerebrospinal Fluid and Urine

    Methods to Study CSF

    HRE Interpretation for Urine

    References

    6. Immunofixation Technique

    Principles of Immunoprecipitation

    Double Diffusion in Two Directions (Ouchterlony Technique)

    Immunoelectrophoresis

    Limitations of Immunoelectrophoresis

    Immunofixation Electrophoresis

    Limitations of Immunofixation

    References

    7. Conditions Associated With Monoclonal Gammopathies

    Ontogeny of B Lymphocytes

    T-Lymphocyte Development

    Conditions Associated With Monoclonal Gammopathies

    References

    8. Laboratory Diagnosis of Monoclonal Gammopathies

    Kappa/Lambda Quantification and the Diagnosis of Monoclonal Gammopathies

    Formulas and Rules as Guides for Detecting Monoclonal Gammopathies

    Clues to Detecting Monoclonal Gammopathies

    Maintaining an Active File on All Patients With Monoclonal Proteins

    Screening and Follow-Up of Bence Jones Protein

    Monoclonals That May Be Difficult to Diagnose

    Final Words

    References

    9. Case Studies for Interpretation

    Introduction to the Case Studies

    General Comments About Interpretation

Product details

  • No. of pages: 314
  • Language: English
  • Copyright: © Butterworth-Heinemann 1994
  • Published: September 21, 1994
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483221090

About the Author

David F. Keren

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