High-Performance Liquid Chromatography

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 5 presents the applications of high-performance liquid chromatography to the analysis and purification of biopolymers. The book, composed of three chapters, provides a detailed description of silica gel-supported stationary phases; tackles biospecific interaction chromatography, a tool in the study of complex carbohydrates and as an industrial separation process in biotechnology; and discusses the potential advantages of displacement chromatography for multicomponent separations. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good source of information.

Contents

Conspectus

Preface

Bonded Silica Phases for the Separation of Biopolymers by Means of Column Liquid Chromatography

I. Introduction

II. Silica and Bonded-Phase Chemistry

III. Survey of Commercial Bonded Silica Packings

IV. Brief Chromatographie Characteristics of LC Phase Systems Applied to Biopolymer Separation

V. Conclusion

References

High-Performance Affinity Chromatography

I. Introduction

II. Survey of the Literature of High-Performance

Affinity Chromatography

III. Fundamental Aspects of High-Performance Affinity Chromatography

IV. Preparation of Affinity Chromatographie Supports

V. Detection Methods in High-Performance Affinity Chromatography

VI. Conclusions

VII. The Future

References

High-Performance Displacement Chromatography

I. Introduction

II. Displacement Development

III. Evolution of Displacement Chromatography

IV. Theory

V. Mathematical Treatment of Displacement Chromatography by the h Transformation

VI. Instrumentation

VII. Selection of the Chromatographie System

VIII. Column Regeneration

IX. Planar Displacement Chromatography

X. Selected Applications of Displacement Chromatography

XI. Effect of Operating Parameters

XII. Concluding Remarks

References

Index