High-Performance Liquid Chromatography

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 5 presents the applications of high-performance liquid chromatography to the analysis and purification of biopolymers. The book, composed of three chapters, provides a detailed description of silica gel-supported stationary phases; tackles biospecific interaction chromatography, a tool in the study of complex carbohydrates and as an industrial separation process in biotechnology; and discusses the potential advantages of displacement chromatography for multicomponent separations. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good source of information.

Contents Conspectus Preface Bonded Silica Phases for the Separation of Biopolymers by Means of Column Liquid Chromatography I. Introduction II. Silica and Bonded-Phase Chemistry III. Survey of Commercial Bonded Silica Packings IV. Brief Chromatographie Characteristics of LC Phase Systems Applied to Biopolymer Separation V. Conclusion References High-Performance Affinity Chromatography I. Introduction II. Survey of the Literature of High-Performance Affinity Chromatography III. Fundamental Aspects of High-Performance Affinity Chromatography IV. Preparation of Affinity Chromatographie Supports V. Detection Methods in High-Performance Affinity Chromatography VI. Conclusions VII. The Future References High-Performance Displacement Chromatography I. Introduction II. Displacement Development III. Evolution of Displacement Chromatography IV. Theory V. Mathematical Treatment of Displacement Chromatography by the h Transformation VI. Instrumentation VII. Selection of the Chromatographie System VIII. Column Regeneration IX. Planar Displacement Chromatography X. Selected Applications of Displacement Chromatography XI. Effect of Operating Parameters XII. Concluding Remarks References Index