High-Performance Liquid Chromatography - 1st Edition - ISBN: 9780123122049, 9781483261881

High-Performance Liquid Chromatography

1st Edition

Advances and Perspectives

Editors: Csaba Horváth
eBook ISBN: 9781483261881
Imprint: Academic Press
Published Date: 5th January 1987
Page Count: 332
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Description

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 4 is an authoritative publication that deals with the fundamentals, instrumentation, and applications of high-performance liquid chromatography. The volume contains articles on practical aspects of reversed-phase chromatography in the study of biopolymer separations; characterization of stationary phases and the development of various packing materials; electrochemical detection; and the fundamentals of chromatographic behavior of large molecules. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book an interesting read.

Table of Contents


Contents

Conspectus

Preface

Reversed-Phase Chromatography of Proteins and Nucleic Acids: Practical Considerations

I. Introduction

II. Equipment Requirements

III. Column Selection

IV. Mobile-Phase Effects

V. Other Practical Considerations

VI. Hydrophobic Interaction Chromatography

VII. RP-HPLC of Oligonucleotides

VIII. Summary

References

Mixed-Interaction Stationary Phases in HPLC

I. Introduction

II. Concept of Mixed-Interaction Chromatography

III. Biopolymer Separations

IV. Low-Pressure Chromatography

V. High-Performance Liquid Chromatography

VI. High-Performance Mixed-Mode Chromatography

VII. Conclusions

References

Liquid Chromatography/Electrochemistry

I. Introduction

II. Basic Principles of LCEC

III. Practical Operational Considerations in LCEC

IV. Multipotential Detection in LCEC

V. Selected Applications of Liquid

Chromatography/Electrochemistry

References

High-Performance Liquid Chromatography Separations of Large Molecules: A General Model

I. Introduction

II. Small-Molecule Retention

III. Small-Molecule Band Broadening

IV. Large-Molecule HPLC: Retention Relationships

V. Large-Molecule HPLC: Band Broadening

VI. Separation and Resolution

VII. Non-Well-Behaved Samples

VIII. The Design of Columns for Macromolecular HPLC

IX. Other Models of Macromolecular HPLC

X. Conclusions

Appendix I. Use of "Normal versus Superficial" Mobile-Phase Velocity: Effect on C Term of Knox Equation

Appendix II. Examples of Calculations by the Model of Table VIII

Glossary of Symbols

References

Index

Details

No. of pages:
332
Language:
English
Copyright:
© Academic Press 1986
Published:
Imprint:
Academic Press
eBook ISBN:
9781483261881

About the Editor

Csaba Horváth