High-Performance Liquid Chromatography

High-Performance Liquid Chromatography

Advances and Perspectives

1st Edition - January 5, 1987

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  • Editor: Csaba Horváth
  • eBook ISBN: 9781483261881

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High-Performance Liquid Chromatography: Advances and Perspectives, Volume 4 is an authoritative publication that deals with the fundamentals, instrumentation, and applications of high-performance liquid chromatography. The volume contains articles on practical aspects of reversed-phase chromatography in the study of biopolymer separations; characterization of stationary phases and the development of various packing materials; electrochemical detection; and the fundamentals of chromatographic behavior of large molecules. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book an interesting read.

Table of Contents

  • Contents



    Reversed-Phase Chromatography of Proteins and Nucleic Acids: Practical Considerations

    I. Introduction

    II. Equipment Requirements

    III. Column Selection

    IV. Mobile-Phase Effects

    V. Other Practical Considerations

    VI. Hydrophobic Interaction Chromatography

    VII. RP-HPLC of Oligonucleotides

    VIII. Summary


    Mixed-Interaction Stationary Phases in HPLC

    I. Introduction

    II. Concept of Mixed-Interaction Chromatography

    III. Biopolymer Separations

    IV. Low-Pressure Chromatography

    V. High-Performance Liquid Chromatography

    VI. High-Performance Mixed-Mode Chromatography

    VII. Conclusions


    Liquid Chromatography/Electrochemistry

    I. Introduction

    II. Basic Principles of LCEC

    III. Practical Operational Considerations in LCEC

    IV. Multipotential Detection in LCEC

    V. Selected Applications of Liquid



    High-Performance Liquid Chromatography Separations of Large Molecules: A General Model

    I. Introduction

    II. Small-Molecule Retention

    III. Small-Molecule Band Broadening

    IV. Large-Molecule HPLC: Retention Relationships

    V. Large-Molecule HPLC: Band Broadening

    VI. Separation and Resolution

    VII. Non-Well-Behaved Samples

    VIII. The Design of Columns for Macromolecular HPLC

    IX. Other Models of Macromolecular HPLC

    X. Conclusions

    Appendix I. Use of "Normal versus Superficial" Mobile-Phase Velocity: Effect on C Term of Knox Equation

    Appendix II. Examples of Calculations by the Model of Table VIII

    Glossary of Symbols



Product details

  • No. of pages: 332
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: January 5, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9781483261881

About the Editor

Csaba Horváth

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