High-Performance Liquid Chromatography
Advances and Perspectives
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High-Performance Liquid Chromatography: Advances and Perspectives, Volume 4 is an authoritative publication that deals with the fundamentals, instrumentation, and applications of high-performance liquid chromatography. The volume contains articles on practical aspects of reversed-phase chromatography in the study of biopolymer separations; characterization of stationary phases and the development of various packing materials; electrochemical detection; and the fundamentals of chromatographic behavior of large molecules. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book an interesting read.
Table of Contents
Contents
Conspectus
Preface
Reversed-Phase Chromatography of Proteins and Nucleic Acids: Practical Considerations
I. Introduction
II. Equipment Requirements
III. Column Selection
IV. Mobile-Phase Effects
V. Other Practical Considerations
VI. Hydrophobic Interaction Chromatography
VII. RP-HPLC of Oligonucleotides
VIII. Summary
References
Mixed-Interaction Stationary Phases in HPLC
I. Introduction
II. Concept of Mixed-Interaction Chromatography
III. Biopolymer Separations
IV. Low-Pressure Chromatography
V. High-Performance Liquid Chromatography
VI. High-Performance Mixed-Mode Chromatography
VII. Conclusions
References
Liquid Chromatography/Electrochemistry
I. Introduction
II. Basic Principles of LCEC
III. Practical Operational Considerations in LCEC
IV. Multipotential Detection in LCEC
V. Selected Applications of Liquid
Chromatography/Electrochemistry
References
High-Performance Liquid Chromatography Separations of Large Molecules: A General Model
I. Introduction
II. Small-Molecule Retention
III. Small-Molecule Band Broadening
IV. Large-Molecule HPLC: Retention Relationships
V. Large-Molecule HPLC: Band Broadening
VI. Separation and Resolution
VII. Non-Well-Behaved Samples
VIII. The Design of Columns for Macromolecular HPLC
IX. Other Models of Macromolecular HPLC
X. Conclusions
Appendix I. Use of "Normal versus Superficial" Mobile-Phase Velocity: Effect on C Term of Knox Equation
Appendix II. Examples of Calculations by the Model of Table VIII
Glossary of Symbols
References
Index
Product details
- No. of pages: 332
- Language: English
- Copyright: © Academic Press 1987
- Published: January 5, 1987
- Imprint: Academic Press
- eBook ISBN: 9781483261881
About the Editor
Csaba Horváth
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