High-Performance Liquid Chromatography

High-Performance Liquid Chromatography

Advances and Perspectives

1st Edition - August 28, 1980

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  • Editor: Csaba Horváth
  • eBook ISBN: 9781483262024

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High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.

Table of Contents

  • Contents

    List of Contributors



    Evolution of Liquid Chromatography: A Historical Overview

    I. The Field as We See It Today

    II. The Beginnings

    III. The Dormant Period

    IV. The Rebirth of Chromatography

    V. Variants of Chromatography

    VI. High-Performance Liquid Chromatography

    VII. Postword


    Practical Operation of Bonded-Phase Columns in High-Performance Liquid Chromatography

    I. Introduction

    II. Types of Bonded-Phase Chromatography

    III. Characteristics of Silica Gel

    IV. Preparation of Bonded Phases

    V. Phase Chain Length and Its Effect

    VI. Columns for Bonded-Phase Chromatography

    VII. Columns for Normal Bonded-Phase Chromatography

    VIII. Modes of Reverse-Phase Chromatography

    IX. Columns for Reverse-Phase Chromatography

    X. Ion-Pair Chromatography

    XI. Other Experimental Variables in Bonded-Phase


    XII. Conclusions


    Secondary Chemical Equilibria in High-Performance Liquid Chromatography

    I. Introduction

    II. Fundamental Concepts

    III. Chemical Principles of Ionic Equilibria

    IV. Chromatography

    V. Conclusion


    Gradient Elution

    I. Introduction

    II. A Simplified Picture of Separation in Gradient Elution

    III. Experimental Considerations

    IV. Designing and Improving a Gradient Separation

    V. Theoretical Relationships

    VI. Conclusion

    List of Symbols



Product details

  • No. of pages: 342
  • Language: English
  • Copyright: © Academic Press 1980
  • Published: August 28, 1980
  • Imprint: Academic Press
  • eBook ISBN: 9781483262024

About the Editor

Csaba Horváth

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