High Performance Liquid Chromatography

High Performance Liquid Chromatography

1st Edition - April 23, 1987

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  • Author: Mary T. Gilbert
  • eBook ISBN: 9781483281292

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High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Table of Contents

  • List of Abbreviations

    Glossary of Symbols

    1 Introduction

    2 Basic Chromatographic Theory

    2.1 Column Capacity Ratio

    2.2 Efficiency

    2.3 Resolution

    2.4 Asymmetry

    3 Equipment

    3.1 Pumps and Gradient Systems

    3.2 Injectors

    3.3 Connectors

    3.4 Detectors

    3.5 Data Systems

    4 The Column

    4.1 Column Design

    4.2 Packing Materials

    4.3 Column Packing and Testing

    4.4 Practical Considerations

    4.5 Coupling of Columns

    4.6 Micro Columns

    5 Sample Treatment

    5.1 Clean-Up Procedures

    5.2 Trace Analysis

    5.3 Derivatisation

    5.4 Post-Column Reaction Detectors

    6 Separation Methods

    6.1 Selection/Optimization of Conditions

    6.2 Normal Phase Chromatography

    6.3 Liquid-Liquid Partition Chromatography

    6.4 Reversed Phase Chromatography

    6.5 Ion Exchange Chromatography

    6.6 Size Exclusion Chromatography

    6.7 Affinity Chromatography

    6.8 Inclusion Chromatography

    6.9 Chiral Chromatography

    7 Adjustment of Selectivity by Use of Fluent Additives

    7.1 Ion Pair Partition Chromatography

    7.2 Chromatography on Dynamically Modified Oxide Gels

    7.3 Metal Complexation

    7.4 Crown Ethers

    8 Preparative Liquid Chromatography

    8.1 Separation Strategy

    8.2 Equipment for Preparative LC

    8.3 Materials for Preparative Chromatography

    8.4 Applications

    9 Chiral Chromatography

    9.1 Enantiomeric Resolution by Preparation of Diastereoisomer

    9.2 Direct Enantiomer Resolution

    10 Current Trends in the Practice of HPLC

    10.1 Columns

    10.2 Detectors

    10.3 Packing Materials

    10.4 Supercritical Fluid Chromatography

    10.5 Conclusions


    Appendix I Packing Materials for High Performance Liquid Chromatography

    Appendix II Properties of Chromatographic Solvents


Product details

  • No. of pages: 396
  • Language: English
  • Copyright: © Butterworth-Heinemann 1987
  • Published: April 23, 1987
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483281292

About the Author

Mary T. Gilbert

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