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High Performance Liquid Chromatography - 1st Edition - ISBN: 9780723608974, 9781483281292

High Performance Liquid Chromatography

1st Edition

Author: Mary T. Gilbert
eBook ISBN: 9781483281292
Imprint: Butterworth-Heinemann
Published Date: 23rd April 1987
Page Count: 396
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High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Table of Contents

List of Abbreviations

Glossary of Symbols

1 Introduction

2 Basic Chromatographic Theory

2.1 Column Capacity Ratio

2.2 Efficiency

2.3 Resolution

2.4 Asymmetry

3 Equipment

3.1 Pumps and Gradient Systems

3.2 Injectors

3.3 Connectors

3.4 Detectors

3.5 Data Systems

4 The Column

4.1 Column Design

4.2 Packing Materials

4.3 Column Packing and Testing

4.4 Practical Considerations

4.5 Coupling of Columns

4.6 Micro Columns

5 Sample Treatment

5.1 Clean-Up Procedures

5.2 Trace Analysis

5.3 Derivatisation

5.4 Post-Column Reaction Detectors

6 Separation Methods

6.1 Selection/Optimization of Conditions

6.2 Normal Phase Chromatography

6.3 Liquid-Liquid Partition Chromatography

6.4 Reversed Phase Chromatography

6.5 Ion Exchange Chromatography

6.6 Size Exclusion Chromatography

6.7 Affinity Chromatography

6.8 Inclusion Chromatography

6.9 Chiral Chromatography

7 Adjustment of Selectivity by Use of Fluent Additives

7.1 Ion Pair Partition Chromatography

7.2 Chromatography on Dynamically Modified Oxide Gels

7.3 Metal Complexation

7.4 Crown Ethers

8 Preparative Liquid Chromatography

8.1 Separation Strategy

8.2 Equipment for Preparative LC

8.3 Materials for Preparative Chromatography

8.4 Applications

9 Chiral Chromatography

9.1 Enantiomeric Resolution by Preparation of Diastereoisomer

9.2 Direct Enantiomer Resolution

10 Current Trends in the Practice of HPLC

10.1 Columns

10.2 Detectors

10.3 Packing Materials

10.4 Supercritical Fluid Chromatography

10.5 Conclusions


Appendix I Packing Materials for High Performance Liquid Chromatography

Appendix II Properties of Chromatographic Solvents



No. of pages:
© Butterworth-Heinemann 1987
23rd April 1987
eBook ISBN:

About the Author

Mary T. Gilbert

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