High-Density Sequencing Applications in Microbial Molecular Genetics - 1st Edition - ISBN: 9780128159934

High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612

1st Edition

Hardcover ISBN: 9780128159934
Imprint: Academic Press
Published Date: 1st December 2018
Page Count: 412
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Table of Contents

1. Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq.
Cecilia Arraiano
2. Conformational studies of bacterial chromosomes by high-throughput sequencing methods
Frederic Boccard
3. Measuring mRNA degradation: delay or not delay
Muriel Cocaign-Bousquet
4. Toxin-antitoxin systems in Helicobacter pylori
Fabien Darfeuille
5. The challenges of genome-wide analyses in a eukaryotic microorganism with two nuclear genomes
Sandra Duharcourt
6. Global recognition patterns of bacterial RNA-binding proteins
Erik Holmqvist
7. High-resolution profiling of NMD targets
Allan Jacobson
8. Generation of a metagenomic 3C/Hi-C library of human gut microbiota
Romain Koszul
9. Genome-wide mapping of yeast retrotransposons integration target sites
Pascale Lesage
10. Measuring protein synthesis rates
Gene-Wei Li
11. Finding unsuspected partners of small RNAs with new screening approaches
Eric Masse
12. Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output
Bryce Nikels
13. RNA-based control of quorum sensing in Vibrio cholerae
Kai Papenfort
14. The role of membrane tethering in the initiation of mRNA decay in Bacillus subtilis
Harald Putzer
15. RNA 5’ end mapping and phosphorylation state in Staphylococcus aureus
Peter Redder
16. Identification of RNA targets of Staphylococcus aureus RNase III combining catalytically inactive mutant enzyme and RNA sequencing
Pascale Romby
17. Non-coding RNAs in Archaea: What do we know today?
Ruth Schmitz
18. Ribosome profiling of the Caulobacter cell cycle
Jared Schrader
19. High-throughput analysis of protein-RNA and RNA-RNA interactions in pathogenic bacteria
Jai Tree
20. Profiling RNA polymerase II modifications genome-wide in fission yeast
Lidia Vasiljeva
21. Multi-organism RNA-seq approaches to bacterial infections
Alexander J. Westermann
22. Sort-Seq in the analysis of genetic regulatory sequences
Wade C. Winkler
23. Genomic analysis of DNA double-strand break repair in Escherichia coli
David Leach


Description

High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612 in the Methods of Enzymology series provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology. Methods permitting tens of millions of sequence reads in a single experiment have paved the way to genome-wide studies that are contributing to our understanding of the complexity of living systems. Chapters in this updated volume include Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq., Conformational studies of bacterial chromosomes by high-throughput sequencing methods, Measuring mRNA degradation, and more.

Addition sections cover Global recognition patterns of bacterial RNA-binding proteins, High-resolution profiling of NMD targets, and the Generation of a metagenomic 3C/Hi-C library of human gut microbiota, Genome-wide mapping of yeast retrotransposons integration target sites, Measuring protein synthesis rates, Finding unsuspected partners of small RNAs with new screening approaches, Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output, RNA-based control of quorum sensing in Vibrio cholerae, amongst other highly regarded topics.

Key Features

  • Detail methods used in research articles that were recently published in leading journals
  • Provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology

Readership

Students and researchers interested in using high-density sequencing methods as a tool for exploring the complexity of living systems. In addition to detailing protocols for a variety of applications in microbial molecular genetics, this volume will help a new generation of researchers to innovate by applying this technology to their research projects


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2018
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128159934

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