Description

This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered.

Readership

Biochemists, molecular biologists, neurophysiologists, neurochemists, cell biologists, pharmacologists, and neuroendocrinologists.

Table of Contents

Ga Subunits: T. Wieland and K.H. Jakobs, Measurement of Receptor-Stimulated Guanosine 5'-O-((-Thio)triphosphate Binding by G Proteins. P. Gierschik, T. Bouillon, and K.H. Jakobs, Receptor-Stimulated Hydrolysis of Guanosine 5'-Triphosphate in Membrane Preparations. E.M. Ross and T. Higashijima, Regulation of G-Protein Activation by Mastoparans and Other Cationic Peptides. D.J. Carty and R. Iyengar, Guanosine 5'-O-((-Thio)triphosphate Binding Assay for Solubilized G Proteins. J. Moss, R.S. Haun, S.-C. Tsai, C.F. Welsh, F.-J.S. Lee, S.R. Price, and M. Vaughan, Activation of Cholera Toxin by ADP-Ribosylation Factors: 20-kDa Guanine Nucleotide-Binding Proteins. D.J. Carty, Pertussis Toxin-Catalyzed ADP-Ribosylation of G Proteins. R.R. Vaillancourt, N. Dhanasekaran, and A.E. Ruoho, Synthesis and Use of Radioactive Photoactivatable NAD+ Derivatives as Probes for G-Protein Structure. M.M. Rasenick, M. Talluri, and W.J. Dunn, III, Photoaffinity Guanosine 5'-Triphosphate Analogs as a Tool for the Study of GTP-Binding Proteins. L. Birnbaumer, D. Grenet, F. Ribeiro-Neto, and J. Codina, Preparation of Activated ( Subunits of Gs and Gis: From Erythrocyte to Activated Subunit. T. Katada, K. Kontani, A. Inanobe, I. Kobayashi, Y. Ohoka, H. Nishina, and K. Takahashi, Purification and Separation of Closely Related Members of Pertussis Toxin-Substrate G Proteins. J. Bigay and M. Chabre, Purification of Transducin. E. Lee, M.E. Linder, and A.G. Gilman, Expression of G-Protein a Subunits in Escherichia coli. I.-H. Pang, A.V. Smrcka, and P.C. Sternweis, Synthesis and Applications of Affinity Matrix Containing Immobilized (( Subunits of G Proteins.

Details

No. of pages:
561
Language:
English
Copyright:
© 1994
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883502
Print ISBN:
9780121821388

About the serial-volume-editor

Ravi Iyengar

Affiliations and Expertise

Mount Sinai School of Medicine, New York, U.S.A.

Reviews

@from:Praise for the Volume @qu:"This is another welcome addition to the excellent 'Methods in Enzymology series... The 'Methods in Enzymology series represents the gold-standard, but even in this context the present volume is special. The list of contributors is impressive, the wide coverage of the field fills a much-needed gap... The best book of its kind on the market. This volume therefore represents extremely good value for money, especially given the relatively long shelf-life of methodology books." @source:--NEUROSCIENCE @qu:"I suspect my copy will rapidly acquire the battered look common to well-used techniques manuals once I release it to the laboratory." @source:--Patrick J. Casey in TRENDS IN CELL BIOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROB