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Heterotrimeric G Proteins - 1st Edition - ISBN: 9780121821388, 9780080883502

Heterotrimeric G Proteins, Volume 237

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Ravi Iyengar
Hardcover ISBN: 9780121821388
eBook ISBN: 9780080883502
Imprint: Academic Press
Published Date: 14th June 1994
Page Count: 561
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Table of Contents

Ga Subunits: T. Wieland and K.H. Jakobs, Measurement of Receptor-Stimulated Guanosine 5'-O-((-Thio)triphosphate Binding by G Proteins. P. Gierschik, T. Bouillon, and K.H. Jakobs, Receptor-Stimulated Hydrolysis of Guanosine 5'-Triphosphate in Membrane Preparations. E.M. Ross and T. Higashijima, Regulation of G-Protein Activation by Mastoparans and Other Cationic Peptides. D.J. Carty and R. Iyengar, Guanosine 5'-O-((-Thio)triphosphate Binding Assay for Solubilized G Proteins. J. Moss, R.S. Haun, S.-C. Tsai, C.F. Welsh, F.-J.S. Lee, S.R. Price, and M. Vaughan, Activation of Cholera Toxin by ADP-Ribosylation Factors: 20-kDa Guanine Nucleotide-Binding Proteins. D.J. Carty, Pertussis Toxin-Catalyzed ADP-Ribosylation of G Proteins. R.R. Vaillancourt, N. Dhanasekaran, and A.E. Ruoho, Synthesis and Use of Radioactive Photoactivatable NAD+ Derivatives as Probes for G-Protein Structure. M.M. Rasenick, M. Talluri, and W.J. Dunn, III, Photoaffinity Guanosine 5'-Triphosphate Analogs as a Tool for the Study of GTP-Binding Proteins. L. Birnbaumer, D. Grenet, F. Ribeiro-Neto, and J. Codina, Preparation of Activated ( Subunits of Gs and Gis: From Erythrocyte to Activated Subunit. T. Katada, K. Kontani, A. Inanobe, I. Kobayashi, Y. Ohoka, H. Nishina, and K. Takahashi, Purification and Separation of Closely Related Members of Pertussis Toxin-Substrate G Proteins. J. Bigay and M. Chabre, Purification of Transducin. E. Lee, M.E. Linder, and A.G. Gilman, Expression of G-Protein a Subunits in Escherichia coli. I.-H. Pang, A.V. Smrcka, and P.C. Sternweis, Synthesis and Applications of Affinity Matrix Containing Immobilized (( Subunits of G Proteins. J.L. Blank and J.H. Exton, Purification of Activated and Heterotrimeric Forms of Gq Proteins. G.L. Waldo, J.L. Boyer, and T.K. Harden, Purification of Phospholipase C-Activating G Protein, G11, from Turkey Erythrocytes. J.R. Hepler, T. Kozasa, and A.G. Gilman, Purification of Recombinant Gqa, G11a, and G16a from Sf9 Cells. S.G. Graber, R.A. Figler, and J.C. Garrison, Expression and Purification of G-Protein a Subunits Using Baculovirus Expression System. E.J. Neer, B.M. Denker, T.C. Thomas, and C.J. Schmidt, Analysis of G-Protein a and (Subunits by in Vitro Translation. Y. Audigier, Assays for Studying Functional Properties of in Vitro Translated Gsa Subunit. S.M. Mumby and M.E. Linder, Myristoylation of G- Protein a Subunits. G. Milligan, Specificity and Functional Applications of Antipeptide Antisera Which Identify G-Protein a Subunits. K.-L. Laugwitz, K. Spicher, G. Schultz, and S. Offermanns, Identification of Receptor-Activated G Proteins: Selective Immunoprecipitation of Photolabeled G-Protein a Subunits. J. Lyons, Identification of Mutant Forms of G-Protein a Subunits in Human Neoplasia by Polymerase Chain Reaction-Based Techniques. P.V. Gejman and L.S. Weinstein, Detection of Mutations and Polymorphisms of the Gsa Subunit Gene by Denaturing Gradient Gel Electrophoresis. S. Winitz, M. Russell, and G.L. Johnson, Construction of Mutant and Chimeric G-Protein a Subunits. T.M. Wilkie, A.M. Aragay, A.J. Watson, and M.I. Simon, Design of Degenerate Oligonucleotide Primers for Cloning of G-Protein a Subunits. C. Kleuss, G. Schultz, and B. Wittig, Microinjection of Antisense Oligonucleotides to Assess G-Protein Subunit Function. R.M. Mortensen and J.G. Seidman, Inactivation of G-protein Genes: Double Knockout in Cell Lines. U. Rudolph, A. Bradley, and L. Birnbaumer, Targeted Inactivation of the Gi2( Gene with Replacement and Insertion Vectors: Analysis in a 96-Well Plate Format. B.E. Snaar-Jagalska and P.J.M. Van Haastert, G-Protein Assays in Dictyostelium. R.A. Cerione, Fluorescence Assays for G-Protein Interactions. H.E. Hamm and H.M. Rarick, Specific Peptide Probes for G-Protein Interactions with Receptors. F. Quan and M. Forte, Vaccinia Virus Systems for Expression of G( Genes in S49 Cells. Gbg Subunits: J. Bigay and M. Chabre, Purification of Tbg Subunit of Transducin. J. Chen, D.J. Carty, and R. Iyengar, Adenylyl Cyclase Assay for bg Subunits of G Proteins. J. Dingus, M.D. Wilcox, R. Kohnken, and J.D. Hildebrandt, Synthesis and Use of Biotinylated bg Complexes Prepared from Bovine Brain G Proteins. C. Gallagher and N. Gautam, Design of Oligonucleotide Probes for Molecular Cloning of b and g Subunits. A.N. Pronin and N. Gautam, Characterization of Antibodies for Various G-Protein b and g Subunits. J.D. Robishaw and E.A. Balcueva, Preparation, Characterization, and Use of Antibodies with Specificity for G-Protein g Subunits. B.K.-K. Fung, J.S. Anant, W.-C. Lin, O.C. Ong, and H.K. Yamane, Isoprenylation of g Subunits and G-Protein Effectors. Author Index. Subject Index.


This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered.


Biochemists, molecular biologists, neurophysiologists, neurochemists, cell biologists, pharmacologists, and neuroendocrinologists.


No. of pages:
© Academic Press 1994
14th June 1994
Academic Press
Hardcover ISBN:
eBook ISBN:


@from:Praise for the Volume @qu:"This is another welcome addition to the excellent 'Methods in Enzymology series... The 'Methods in Enzymology series represents the gold-standard, but even in this context the present volume is special. The list of contributors is impressive, the wide coverage of the field fills a much-needed gap... The best book of its kind on the market. This volume therefore represents extremely good value for money, especially given the relatively long shelf-life of methodology books." @source:--NEUROSCIENCE @qu:"I suspect my copy will rapidly acquire the battered look common to well-used techniques manuals once I release it to the laboratory." @source:--Patrick J. Casey in TRENDS IN CELL BIOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

Ratings and Reviews

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Ravi Iyengar

Affiliations and Expertise

Mount Sinai School of Medicine, New York, U.S.A.