A. Tar, T.D. Ting, and Y.-K. Ho, Purification of Bovine Retinal cGMP Phosphodiesterase.
H.M. Rarick, N.O. Artemyev, J.S. Mills, N.P. Skiba, and H.E. Hamm, Specific Peptide Probes for G-Protein Interaction with Effectors. Adenylyl Cyclases:
R.A. Johnson, R. Alvarez, and Y. Salomon, Determination of Adenylyl Cyclase Catalytic Activity Using Single and Double Column Procedures.
R.A. Johnson and I. Shoshani, Preparation and Use of P-Site-Targeted Affinity Ligands for Adenylyl Cyclases.
D.M.F. Cooper, Regulation of Ca2f-Sensitive Adenylyl Cyclases by Calcium in Vitro and in Vivo.
Y.H. Wong, Gi Assays in Transfected Cells.
R. Taussig, W.-J. Tang, and A.G. Gilman, Expression and Purification of Recombinant Adenylyl Cyclases in Sf9 Cells.
O. Jacobowitz, J. Chen, and R. Iyengar, Transient Expression Assays for Mammalian Adenylyl Cyclases.
R.T. Premont, Identification of Adenylyl Cyclases by Amplification Using Degenerate Primers. Phospholipases C:
M. De Vivo, Assays for G-Protein Regulation of Phospholipase C Activity.
E.M. Landau and R.D. Blitzer, Chloride Current Assay for Phospholipase C in XenopusOocytes.
S. Cockcroft, G.M.H. Thomas, E. Cunningham, and A. Ball, Use of Cytosol-Depleted HL60 Cells for Reconstitution Studies of G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes.
G.M.H. Thomas, E. Cunningham, and S. Cockcroft, Purification of Phosphatidylinositol Transfer Protein from Brain Cytosol for Reconstituting G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes.
P. Gierschik and M. Camps, Stimulation of Phospholipa
This volume of Methods in Enzymology and its companion Volume 237 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins and their effectors. Methods unique to signal transducing G proteins and general techniques that have been applied to the study of G protein systems are covered.
Biochemists, molecular biologists, neurophysiologists,
- No. of pages:
- © Academic Press 1994
- 8th September 1994
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY
Mount Sinai School of Medicine, New York, U.S.A.
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA