Heterotrimeric G-Protein Effectors, Volume 238

1st Edition

Serial Volume Editors: Ravi Iyengar
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821395
eBook ISBN: 9780080883519
Imprint: Academic Press
Published Date: 8th September 1994
Page Count: 456


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Table of Contents

cGMP Phosphodiesterase:

A. Tar, T.D. Ting, and Y.-K. Ho, Purification of Bovine Retinal cGMP Phosphodiesterase.

H.M. Rarick, N.O. Artemyev, J.S. Mills, N.P. Skiba, and H.E. Hamm, Specific Peptide Probes for G-Protein Interaction with Effectors. Adenylyl Cyclases:

R.A. Johnson, R. Alvarez, and Y. Salomon, Determination of Adenylyl Cyclase Catalytic Activity Using Single and Double Column Procedures.

R.A. Johnson and I. Shoshani, Preparation and Use of P-Site-Targeted Affinity Ligands for Adenylyl Cyclases.

D.M.F. Cooper, Regulation of Ca2f-Sensitive Adenylyl Cyclases by Calcium in Vitro and in Vivo.

Y.H. Wong, Gi Assays in Transfected Cells.

R. Taussig, W.-J. Tang, and A.G. Gilman, Expression and Purification of Recombinant Adenylyl Cyclases in Sf9 Cells.

O. Jacobowitz, J. Chen, and R. Iyengar, Transient Expression Assays for Mammalian Adenylyl Cyclases.

R.T. Premont, Identification of Adenylyl Cyclases by Amplification Using Degenerate Primers. Phospholipases C:

M. De Vivo, Assays for G-Protein Regulation of Phospholipase C Activity.

E.M. Landau and R.D. Blitzer, Chloride Current Assay for Phospholipase C in XenopusOocytes.

S. Cockcroft, G.M.H. Thomas, E. Cunningham, and A. Ball, Use of Cytosol-Depleted HL60 Cells for Reconstitution Studies of G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes.

G.M.H. Thomas, E. Cunningham, and S. Cockcroft, Purification of Phosphatidylinositol Transfer Protein from Brain Cytosol for Reconstituting G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes.

P. Gierschik and M. Camps, Stimulation of Phospholipa


This volume of Methods in Enzymology and its companion Volume 237 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins and their effectors. Methods unique to signal transducing G proteins and general techniques that have been applied to the study of G protein systems are covered.


Biochemists, molecular biologists, neurophysiologists,


No. of pages:
© Academic Press 1994
Academic Press
eBook ISBN:
Hardcover ISBN:


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About the Serial Volume Editors

Ravi Iyengar Serial Volume Editor

Affiliations and Expertise

Mount Sinai School of Medicine, New York, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA