This volume of Methods in Enzymology and its companion Volume 237 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins and their effectors. Methods unique to signal transducing G proteins and general techniques that have been applied to the study of G protein systems are covered.


Biochemists, molecular biologists, neurophysiologists,

Table of Contents

cGMP Phosphodiesterase: A. Tar, T.D. Ting, and Y.-K. Ho, Purification of Bovine Retinal cGMP Phosphodiesterase. H.M. Rarick, N.O. Artemyev, J.S. Mills, N.P. Skiba, and H.E. Hamm, Specific Peptide Probes for G-Protein Interaction with Effectors. Adenylyl Cyclases: R.A. Johnson, R. Alvarez, and Y. Salomon, Determination of Adenylyl Cyclase Catalytic Activity Using Single and Double Column Procedures. R.A. Johnson and I. Shoshani, Preparation and Use of P-Site-Targeted Affinity Ligands for Adenylyl Cyclases. D.M.F. Cooper, Regulation of Ca2f-Sensitive Adenylyl Cyclases by Calcium in Vitro and in Vivo. Y.H. Wong, Gi Assays in Transfected Cells. R. Taussig, W.-J. Tang, and A.G. Gilman, Expression and Purification of Recombinant Adenylyl Cyclases in Sf9 Cells. O. Jacobowitz, J. Chen, and R. Iyengar, Transient Expression Assays for Mammalian Adenylyl Cyclases. R.T. Premont, Identification of Adenylyl Cyclases by Amplification Using Degenerate Primers. Phospholipases C: M. De Vivo, Assays for G-Protein Regulation of Phospholipase C Activity. E.M. Landau and R.D. Blitzer, Chloride Current Assay for Phospholipase C in XenopusOocytes. S. Cockcroft, G.M.H. Thomas, E. Cunningham, and A. Ball, Use of Cytosol-Depleted HL60 Cells for Reconstitution Studies of G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes. G.M.H. Thomas, E. Cunningham, and S. Cockcroft, Purification of Phosphatidylinositol Transfer Protein from Brain Cytosol for Reconstituting G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes. P. Gierschik and M. Camps, Stimulation of Phospholipa


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Academic Press
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