Hemoglobins, Part C: Biophysical Methods - 1st Edition - ISBN: 9780121821333, 9780080883458

Hemoglobins, Part C: Biophysical Methods, Volume 232

1st Edition

Serial Volume Editors: Johannes Everse Kim Vandegriff
Editor-in-Chiefs: John Abelson Melvin Simon
eBook ISBN: 9780080883458
Imprint: Academic Press
Published Date: 25th April 1994
Page Count: 725


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Table of Contents

Molecular Structure and Dynamics:

Y. Goto and A.L. Fink, Acid-Induced Folding of Heme Proteins.

R. Liddington, X-Ray Crystallography of Partially Liganded Structures.

S.W. Englander and J.J. Englander, Structure and Energy Change in Hemoglobin by Hydrogen Exchange Labeling.

S. Pin and C.A. Royer, High-Pressure Fluorescence Methods for Observing Subunit Dissociation in Hemoglobin.

A. Bellelli and M. Brunori, Optical Measurements of QuaternaryStructural Changes in Hemoglobin.

M.C. Marden, J. Kister, and C. Poyart, Allosteric Equilibirum Measurements with Hemoglobin Valency Hybrids.

G. McLendon and J. Feitelson,Electron-Transfer Reactions of Hemoglobin with Small Molecules: A Potential Probe of Conformational Dynamics. Spectroscopy:

C. Ho and J.R. Perussi, Proton Nuclear Magnetic Resonance Studies of Hemoglobin.

A. Dong and W.S. Caughey, Infrared Methods for Study of Hemoglobin Reactions and Structures.

R. H. Austin and L. J. Rothberg, Picosecond Infrared Spectroscopy of Hemoglobin and Myoglobin.

J.M. Friedman, Time-Resolved Resonance Raman Spectroscopy as Probe of Structure, Dynamics, and Reactivity in Hemoglobin.

R.E. Hirsch, Front-Face Fluorescence Spectroscopy of Hemoglobins.

C. Zentz, S. Pin, and B. Alpert, Stationary and Time-Resolved Circular Dichroism of Hemoglobins.

S. Pin, B. Alpert, A. Congiu-Castellano, S.D. Longa, and A. Bianconi, X-Ray Absorption Spectroscopy of Hemoglobin.

F.A. Ferrone, Modulated Excitation Spectroscopy in Hemoglobin.

J. Deak, L. Richard, M. Pereira, H.-L. Chui, and R.J.D. Miller, Picosecond Phase Grating Spectroscopy: Applications to Bioenergetics and Protein Dynamics.

Ligand Binding:

A.J. Mathews and J.S. Olson, Assignment of Rate Constants for O2 and CO Binding to a and b Subunits within R- and T-State Human Hemoglobin.

J. Hofrichter, A. Ansari, C.M. Jones, R.M. Deutsch, J.H. Sommer, and E.R. Henry, Ligand Binding and Conformational Changes Measured by Time-Resolved Absorption Spectroscopy.

J.-L. Martin and M.H. Vos, Femtosecond Measurements of Geminate Recombination in Heme Proteins.

V.S. Sharma, Double Mixing Methods for Kinetic Studies of Ligand Binding inPartially Liganded Intermediates of Hemoglobin.

M. Perrella and L. Rossi-Bernardi, Hemoglobin-Liganded Intermediates.

K.D. Vandegriff and R.I. Shrager, Hemoglobin-Oxygen Equilibrium Binding: Rapid-Scanning Spectrophotometry and Singular Value Decomposition.

R.M. Winslow, A. Murray, and C.C. Gibson, Oxygen Equilibrium Curve of Concentrated Hemoglobin.

C. Poyart, M.C. Marden, and J. Kister, Bezafibrate Derivatives as Potent Effectors of Hemoglobin.

Mathematical Analysis and Modeling:

R.L. Berger, N. Davids, and M. Perrella, Simulation of Hemoglobin Kinetics Using Finite Element Numerical Methods.

K. Imai, Adair Fitting to Oxygen Equilibrium Curves of Hemoglobin.

M.L. Doyle, D.W. Myers, G.K. Ackers, and R. I. Shrager, Weighted Nonlinear Regression Analysis of Highly Cooperative Oxygen Equilibrium Curves.

M.L. Johnson, Dimer-Tetramer Equilibrium in Adair Fitting.

L. J. Parkhurst, T.M. Larsen, and H.-Y. Lee, Effects of Wavelength on Fitting Adair Constants for Binding of Oxygen to Human Hemoglobin.

E.C. DeLand, Adair Equation: Rederiving Oxygenation Parameters.

E. Di Cera, Linkage Thermodynamics. Author Index. Subject Index.


Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoglobin is again central to a new area: Development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion Volume 231 are indispensable to anyone with a serious interest in this emerging field. They completely updated and extended the information presented in Volume 76, which was published more than 10 years ago.

Key Features

@introbul:Key Features @bul:* Molecular structure and dynamics

  • Spectroscopy
  • Ligand binding
  • Mathematical analysis and modeling


Biochemists, molecular biologists, cell biologists, physiologists, pathologists, biophysicists, clinical chemists, industrial and academic researchers involved in the preparation of hemoglobin derivatives and in the preparationand analysis of recombinant proteins.


No. of pages:
© Academic Press 1994
Academic Press
eBook ISBN:


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About the Serial Volume Editors

Johannes Everse Serial Volume Editor

Affiliations and Expertise

School of Medicine, Texas Tech University Health Science Center, Lubbock, U.S.A.

Kim Vandegriff Serial Volume Editor

Affiliations and Expertise

University of California, Department of Medicine and Veterans Affairs Medical Center, San Diego, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA