Hemoglobins, Part C: Biophysical Methods, Volume 232

1st Edition

Serial Volume Editors: Johannes Everse Kim Vandegriff
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821333
eBook ISBN: 9780080883458
Imprint: Academic Press
Published Date: 25th April 1994
Page Count: 725


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Table of Contents

Molecular Structure and Dynamics:

Y. Goto and A.L. Fink, Acid-Induced Folding of Heme Proteins.

R. Liddington, X-Ray Crystallography of Partially Liganded Structures.

S.W. Englander and J.J. Englander, Structure and Energy Change in Hemoglobin by Hydrogen Exchange Labeling.

S. Pin and C.A. Royer, High-Pressure Fluorescence Methods for Observing Subunit Dissociation in Hemoglobin.

A. Bellelli and M. Brunori, Optical Measurements of QuaternaryStructural Changes in Hemoglobin.

M.C. Marden, J. Kister, and C. Poyart, Allosteric Equilibirum Measurements with Hemoglobin Valency Hybrids.

G. McLendon and J. Feitelson,Electron-Transfer Reactions of Hemoglobin with Small Molecules: A Potential Probe of Conformational Dynamics. Spectroscopy:

C. Ho and J.R. Perussi, Proton Nuclear Magnetic Resonance Studies of Hemoglobin.

A. Dong and W.S. Caughey, Infrared Methods for Study of Hemoglobin Reactions and Structures.

R. H. Austin and L. J. Rothberg, Picosecond Infrared Spectroscopy of Hemoglobin and Myoglobin.

J.M. Friedman, Time-Resolved Resonance Raman Spectroscopy as Probe of Structure, Dynamics, and Reactivity in Hemoglobin.

R.E. Hirsch, Front-Face Fluorescence Spectroscopy of Hemoglobins.

C. Zentz, S. Pin, and B. Alpert, Stationary and Time-Resolved Circular Dichroism of Hemoglobins.

S. Pin, B. Alpert, A. Congiu-Castellano, S.D. Longa, and A. Bianconi, X-Ray Absorption Spectroscopy of Hemoglobin.

F.A. Ferrone, Modulated Excitation Spectroscopy in Hemoglobin.

J. Deak, L. Richard, M. Pereira, H.-L. Chui, and R.J.D. Miller, Picosecond Phase Grating Spectroscopy: Applications to Bioenergetics and Protei


Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoglobin is again central to a new area: Development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion Volume 231 are indispensable to anyone with a serious interest in this emerging field. They completely updated and extended the information presented in Volume 76, which was published more than 10 years ago.

Key Features

@introbul:Key Features @bul:* Molecular structure and dynamics

  • Spectroscopy
  • Ligand binding
  • Mathematical analysis and modeling


Biochemists, molecular biologists, cell biologists, physiologists, pathologists, biophysicists, clinical chemists, industrial and academic researchers involved in the preparation of hemoglobin derivatives and in the preparationand analysis of recombinant proteins.


No. of pages:
© Academic Press 1994
Academic Press
eBook ISBN:
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About the Serial Volume Editors

Johannes Everse Serial Volume Editor

Affiliations and Expertise

School of Medicine, Texas Tech University Health Science Center, Lubbock, U.S.A.

Kim Vandegriff Serial Volume Editor

Affiliations and Expertise

University of California, Department of Medicine and Veterans Affairs Medical Center, San Diego, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA