Hemoglobins, Part B: Biochemical and Analytical Methods, Volume 231

1st Edition

Print ISBN: 9780121821326
eBook ISBN: 9780080883441
Imprint: Academic Press
Published Date: 30th March 1994
Page Count: 743

Institutional Access


Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago.

Key Features

@introbul:Key Features @bul:* Preparation, purification, and analysis of human hemoglobin and of various non-human hemoglobins

  • Preparation of hemoglobin hybrids and of model heme compounds
  • Preparation of chemically modified hemoglobins and of recombinant hemoglobins
  • Analytical procedures presented for studying hemoglobin stability and degradation and for the formation of xenobiotic adducts of hemoglobin


Biochemists, molecular biologists, cell biologists, physiolgists, pathologists, biophysicists, clinical chemists, industrial and academic researchers involved in the preparation of hemoglobin derivatives and in the preparationand analysis of recombinant proteins.

Table of Contents

Preparation, Purification, and Analysis of Human Hemoglobin:

R.M. Winslow and K.W. Chapman, Pilot-Scale Preparation of Hemoglobin Solutions.

B.J.-M. Thevenin and S.B. Shohet, Detection of Red Cell Membrane Components in Human Hemoglobin Preparations.

B. Masala and L. Manca, Detection of Globin Chains by Reversed-Phase High-Performance Liquid Chromatography.

P. Ferranti, A. Malorni, and P. Pucci, Structural Characterization of Hemoglobin Variants Using Capillary Electrophoresis and Fast Atom Bombardment Mass Spectrometry.

M.P. Cohen and V.-Y. Wu, Purification of Glycated Hemoglobin.

R.I. Roth and J. Levin, Measurement of Endotoxin Levels in Hemoglobin Preparations.

Preparation and Characterization of Nonhuman Hemoglobins:

H.H. Ruf, A.G. Altemaller, and K. Gersonde, Preparation and Characterization of Insect Hemoglobins from Chironomus thummi thummi.

S.N. Vinogradov and P.K. Sharma, Preparation and Characterization of Invertebrate Globin Complexes.

A. DeYoung, L.D. Kwiatkowski, and R.W. Noble, Fish Hemoglobins.

C. Fronticelli and E. Bucci, Conformational and Functional Characteristics of Bovine Hemoglobin.

Hemoglobin Hybrids and Model Hemes:

E. Tsuchida and T. Komatsu, Synthetic Hemes.

R.P. Roy and A.S. Acharya, Semisynthesis of Hemoglobin.

A. Tsuneshige and T. Yonetani, Preparation of Mixed Metal Hybrids.

Chemically Modified Hemoglobins:

J.M. Manning, Preparation of Hemoglobin Derivatives Selectively or Randomly Modified at Amino Groups.

M.J. Rao and A.S. Acharya, Amidation of Basic Carboxyl Groups of Hemoglobin.



No. of pages:
© Academic Press 1994
Academic Press
eBook ISBN:
Hardcover ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY