Helicase Enzymes Part B

Helicase Enzymes Part B

1st Edition - August 15, 2022

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  • Editor: Michael Trakselis
  • Hardcover ISBN: 9780323997737

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Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology serials
  • Updated release includes the latest information on Helicase Enzymes


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Crosslinking immunoprecipitation of Upf1 in yeast
    Kristian Baker
    2. Biochemical Analysis of the SARS-CoV-2 Nsp13 Helicase Implicated in COVID-19
    Robert Brosh
    3. Bulk phase biochemistry of the Pif1 helicase
    Matthew Bochman
    4. Translational control by helicases during cellular stress
    Tim Bolger
    5. Measuring DDX3-dependent protein synthesis in vitro and in cells
    Stephen Floor
    6. Purification of Mtr4 and Mtr4-mediated complexes
    Sean Johnson
    7. Plan of attack for studying DEAD-box proteins
    Katrin Karbstein
    8. Reconstitution, purification, and activity characterization for RNA-guided helicase-nuclease machines in Type I CRISPR-Cas systems
    Ailong Ke
    9. Helicase mediated vectorial folding of G-quadruplex
    Sua Myong
    10. Detecting G4 quadruplex unwinding
    Katrin Paeschke
    11. Monitoring RNA and ATP-dependent conformational changes in RIG-I-like receptors using fluorescence microscopy
    Anna Marie Pyle
    12. ATP utilization measurements in RNA unwinding and RNA chaperone activities by helicase proteins
    Rick Russell
    13. Hydrogen-Deuterium Exchange Mass Spectrometry of RNA Helicases
    Sheena D'Arcy
    14. Biochemical characterization of human MTR4-RNA exosome and adaptor complexes
    Christopher D. Lima
    15. Study of RNA helicases with magnetic tweezers
    Herve Le Hir
    16. Single-molecule FRET to analyze the dynamics of RNA helicases
    Dagmar Klostermeier
    17. Transcriptional regulation by a RecQ helicase
    Sudha Sharma
    18. In vitro characterization of the DEXH helicase Dhr1 from yeast
    Arlen Johnson

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: August 15, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323997737

About the Serial Volume Editor

Michael Trakselis

Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.

Affiliations and Expertise

Associate Professor and Director of Graduate Affairs, Baylor University, TX, USA

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