Helicase Enzymes Part A

Helicase Enzymes Part A

1st Edition - August 1, 2022

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  • Editor: Michael Trakselis
  • Hardcover ISBN: 9780323914765

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Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology serials
  • Updated release includes the latest information on Helicase Enzymes


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Fluorescence loading assay for hexameric helicases
    James Berger
    2. Use of substrates with multiple fluorophores to simultaneously monitor unwinding of multiple duplexes
    Alicia Byrd
    3. In silico reconstitution of MCM helicase loading and activation using time resolved cryo-EM
    Alessandro Costa
    4. Helicase uncoupling to study nacent strand decay
    James Dewar
    5. Investigating structure and dynamics of Twinkle helicase with Cryo-EM and high-speed AFM
    Yang Gao
    6. Measuring helicase contributions to BIR
    Greg Ira
    7. Crosslinking to define helicase/DNA interactions
    James L. Keck 
    8. Use of Reverse Polarity DNA to investigate Helicase Mechanisms
    Tim Lohman
    9. Substrate production to study helicases and translocases using molecular and optical tweezers
    Fernando Moreno Herrero
    10. Methods of loading CMG onto DNA
    Michael O’Donnell
    11. Methods to study Helicase-Polymerase coupling during DNA replication reactions
    Smita Patel
    12. Biochemical analyses of ATP-dependent chromatin remodeling
    Craig Peterson
    13. Alignment of helicases on single-stranded increases activity
    Kevin D. Raney
    14. CMG helicase activity on G4-containing templates
    Dirk Remus
    15. Accessory helicase unwinding of genomic complexities
    Grant Schauer
    16. Monitoring helicase decoupling in bacteria by TUNEL
    Michael Trakselis
    17. the preparation of DNA constructs for the use in single-molecule replisome studies
    Antoine Van Oijen
    18. Interaction of topoisomerases with DNA measured by RADAR
    Alessandro Vindigni

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: August 1, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323914765

About the Serial Volume Editor

Michael Trakselis

Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.

Affiliations and Expertise

Associate Professor and Director of Graduate Affairs, Baylor University, TX, USA

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