The compilation of this book was prompted by the necessity of a bench volume which could provide the necessary background information on materials, experimental design, pitfalls and dificulties, in order to perform a particular test in an acceptable way with a minimal need for additional expert help. This Second Edition updates this information, providing: - a comprehensive bench guide - methods known to be reliable - a broad spectrum of approaches - tips to avoid pitfalls when using unfamiliar techniques - data from population records - safety aspects of mutagens and carcinogens - basic statistical concepts for experiment design This `on the bench' methodological text provides the necessary information for most of the common assays for genetic damage in use. The book includes methods which have been sufficiently used and tested to make their use reliable, but also presents methods which are not widely used at present, but which might prove most useful in screening for mutagenic effects.
Preface to the First Edition Preface to the Second Edition List of Contributors Chapter 1. Newer Methods for Determining Genotoxicity Using DNA Repair-Deficient and Repair-Proficient Escherichia coli Chapter 2. Bacillus Subtilis Repair Test Chapter 3. Methods for Studying Excision Repair of Eukaryotic DNA Damaged by Physical and Chemical Mutagens Chapter 4. Methods for Detecting Carcinogens and Mutagens with the Alkaline Elution/Rat Hepatocyte assay Chapter 5. Induced Reversion Using Human Adenovirus Chapter 6. Revised Methods for the Salmonella Mutagenicity Test Chapter 7. The Fluctuation Test in Bacteria Chapter 8. Mutagen Testing Using TRP+ Reversion in Escherichia coli Chapter 9. Methodologies for the Direct and Animal-Mediated Determination of Various Genetic Effects in Derivatives of Strain 343/113 of Escherichia coli Chapter 10. Basic Principles and Methods of Genotoxicity Testing in the Yeast Saccharomyces Cerevisiae Chapter 11. Procedures for the L5178y/TK+/- → TK-/- Mouse Lymphoma Cell Mutagenicity assay Chapter 12. a Quantitative Test for Mutagenicity in V79 Chinese Hamster Cells Chapter 13. Genetic Markers for Quantitative Mutagenesis Studies in Chinese Hamster Ovary Cells: Applications to Mutagen Screening Studies Chapter 14. Chemical Mutagenesis with Diploid Human Fibroblasts Chapter 15. 6-Thioguanine-Resistant Lymphocytes in Human Blood Chapter 16. The Specific Locus Test in the Mouse Chapter 17. Procedures and Evaluation of Results of the Mouse Spot Test Chapter 18. Human Peripheral Blood Lymphocytes for the Analysis of Chromosome Aberrations in Mutagen Tests Chapter 19. In Vitro Anaphase and Metaphase Preparations in Mutation Testing Chapter 20. The Bone Marrow Micronucleus Test Chapter 21. Cytogenetic Analysis of Structural Chromosome Aberrations and Aneuploidies in Oocytes, Zygotes and Preimplantation Embryos of Mammals Chapter 22. The Dominant Lethal assay in the Male Mouse Chapter 23. Test for Heritable Translocations i
- © Elsevier Science 1984
- 1st January 1984
- Elsevier Science
- eBook ISBN: