Handbook of Histopathological and Histochemical Techniques

Handbook of Histopathological and Histochemical Techniques

Including Museum Techniques

3rd Edition - January 1, 1974

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  • Author: C. F. A. Culling
  • eBook ISBN: 9781483164793

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Handbook of Histopathological and Histochemical Techniques (Including Museum Techniques), Third Edition is a reference guide on techniques used in histology and histochemistry, including museum techniques. This book is comprised of 36 chapters divided into six sections. After giving a brief description of the structure and contents of the cell, this book then turns to the various methods of examination of tissue cells and structures, including the smear method, vital staining, and electron microscopy. The next two sections deal with fixation, decalcification, processing, and section cutting, along with stains, impregnations, and mountants. An exhaustive survey of carbohydrate and protein histochemistry is included. Special procedures such as autoradiography, micro-incineration, and injection techniques are described. The remaining chapters focus on the preparation, mounting, and presentation of museum specimens. The book concludes with an analysis of the microscope. This monograph will be invaluable to all those with an interest in histology and histochemistry, including undergraduate students in the biological and health sciences, pathologists in both the hospital setting and experimental research, and technologists and workers in various research fields.

Table of Contents

  • Foreword

    Preface to Third Edition

    Preface to First Edition


    Part I Introductory

    Chapter 1. The Cell

    Chapter 2. Methods of Examination of Tissues and Cells

    Part II Fixation, Processing and Section Cutting

    Chapter 3. Fixation

    Chapter 4. Decalcification

    Chapter 5. Processing

    Chapter 6. Section Cutting

    Part III Stains, Impregnations and Mountants

    Chapter 7. Theory of Staining

    Chapter 8. Preparation of Stains

    Chapter 9. Mountants

    Chapter 10. Basic Staining and Mounting Procedures

    Chapter 11. Routine Stains

    Chapter 12. Proteins

    Chapter 13. Nucleoproteins—Deoxyribonucleic Acid and Ribonucleic Acid

    Chapter 14. Carbohydrates

    Chapter 15. Enzymes

    Chapter 16. Lipids (Fats, Lipoids, Lipins)

    Chapter 17. Pigments

    Chapter 18. Micro-Organisms

    Chapter 19. Tissues Requiring Special Treatment or Techniques

    Chapter 20. Certain Cytoplasmic Constituents and Cell Products

    Part IV Special Procedures

    Chapter 21. Exfoliative Cytology and Chromosome Techniques

    Chapter 22. Autoradiography

    Chapter 23. Vital Staining

    Chapter 24. Micro-Incineration

    Chapter 25. Injection Techniques

    Part V Museum Technique

    Chapter 26. Preparation, Color Maintenance, Fixation and Storage of Specimens

    Chapter 27. Mounting of Museum Specimens

    Chapter 28. Special Methods

    Chapter 29. Presentation of Museum Specimens

    Part VI The Microscope

    Chapter 30. The Compound Microscope

    Chapter 31. The Dark-Ground Microscope

    Chapter 32. Fluorescence Microscopy

    Chapter 33. The Polarizing Microscope

    Chapter 34. The Phase-Contrast Microscope

    Chapter 35. The Interference Microscope

    Chapter 36. The Electron Microscope



Product details

  • No. of pages: 726
  • Language: English
  • Copyright: © Butterworth-Heinemann 1974
  • Published: January 1, 1974
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483164793

About the Author

C. F. A. Culling

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