Handbook of Endocrine Research Techniques - 1st Edition - ISBN: 9780122099205, 9780080917658

Handbook of Endocrine Research Techniques

1st Edition

Editors: Flora de Pablo Colin Scanes Bruce Weintraub
Hardcover ISBN: 9780122099205
eBook ISBN: 9780080917658
Imprint: Academic Press
Published Date: 22nd October 1993
Page Count: 599
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Description

Written by experts in the field, Handbook of Endocrine Research Techniques is currently the only single source of up-to-date methods and strategies particularly useful in endocrinological research. As a resource for both the novice and experienced investigator, the book includes chapters which provide an introduction to the area, general concepts, detailed protocols, and extensive references.

Table of Contents

Hormone Assays. Radioimmunoassay (RIA): Concept and Types. Immunoradiometric (IRMA) Assays. Enzyme-Linked Immunoabsorbent Assays (ELISA). Development and Characterization of Polyclonal and Monoclonal Antibodies. Isolation of Polypeptide Hormones. Reverse Hemolytic Plaque Assays. Capillary Electrophoresis Coupled to Fluorescence Detection for the Determination of Multiple Neuropeptides. Immunoreactive Heterogeneity in Peptide Measurement. Why Use a Flow Cytometer for Endocrine Cell Analysis? Identification and Characterization of Insulin-Like Growth Factor Binding Proteins. Histochemical and in Situ Approaches. Localization of Peptides. Electron Microscopic Immunocytochemical Approaches to the Localization of Ligands, Receptors, Transducers, And Transporters. In Situ Hybridization for Hormone and Receptor Expression. Techniques for Receptors and Signal Transductors. Plasma Membrane Isolation Strategies for Cell Surface Receptors. Tyrosine Kinase Receptors. Involvement of Protein Tyrosine Phosphatase Activity in the Regulation of Insulin's Signal Transduction. Techniques for Evaluating Protein Kinase C in Intact Cells. Identification and Quantification of G Proteins. Determination of Adenylyl Cyclase Catalytic Activity. Intracellular Mediators of Peptide Hormone Action. Free Calcium Measurements. Molecular Techniques and Specific Model Systems. Approaches for the Purification, Quantitation, And Interpretation of Hormone and Receptor Mrnas. The Use of PCR in Endocrine Research. Detection of Mutations in Hormone Receptor Genes. Techniques to Study Regulation of Gene Expression. Transgenic Animals. The Use of Non-Mammalian Models. Proliferation Induced By Growth Factors in Mammalian Fetal Cells. Analysis of Hormones and Receptors in Embryogenesis.

Details

No. of pages:
599
Language:
English
Copyright:
© Academic Press 1993
Published:
Imprint:
Academic Press
eBook ISBN:
9780080917658
Hardcover ISBN:
9780122099205

About the Editor

Flora de Pablo

Affiliations and Expertise

Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain

Colin Scanes

Affiliations and Expertise

University of Wisconsin, Milwaukee, USA

Bruce Weintraub