COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
GUS Protocols - 1st Edition - ISBN: 9780122740107, 9780323137645

GUS Protocols

1st Edition

Using the GUS Gene as a Reporter of Gene Expression

Editor: Sean Gallagher
eBook ISBN: 9780323137645
Imprint: Academic Press
Published Date: 10th March 1992
Page Count: 221
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.


The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay.

GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.

Key Features

  • First book on the GUS reporter system
  • Up-to-date reference lists following each of the fourteen chapters
  • Comb-bound for convenient bench-top use
  • Written by leading authorities including R.A. Jefferson, inventor of the GUS assay
  • Illustrated with color
  • GUS detection by fluorometric, spectrophotometric, and histochemical methods
  • Fast, automated assays


Graduate students and researchers in molecular biology, biotechnology, molecular genetics, biochemistry, animal cell biology and developmental biology, and plant science

Table of Contents

(Section Headings): The GUS Reporter Gene System. The GUS Assay. Histochemical Detection of GUS. Applications of GUS to Plant Genetic Analysis. Applications of GUS to Animal Genetic Anaylsis. Appendices. Index.


No. of pages:
© Academic Press 1992
10th March 1992
Academic Press
eBook ISBN:

About the Editor

Sean Gallagher

Affiliations and Expertise

Hoefer Scientific Instruments, San Francisco, California, U.S.A.


"This manual does an excellent job of demonstrating both the power and the versatility of the GUS system in plants... If you think the GUS system may be of use to your studies, this text provides a broad range of protocols, practical tips, and limitations to help use the system efficiently." --ANALYTICAL BIOCHEMISTRY

"Gus Protocols is a laboratory manual that will be of great value to the researcher." --TRENDS IN BIOTECHNOLOGY

Ratings and Reviews