GUS Protocols

GUS Protocols

Using the GUS Gene as a Reporter of Gene Expression

1st Edition - March 10, 1992

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  • Editor: Sean Gallagher
  • eBook ISBN: 9780323137645

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Description

The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay. GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.

Key Features

  • First book on the GUS reporter system
  • Up-to-date reference lists following each of the fourteen chapters
  • Comb-bound for convenient bench-top use
  • Written by leading authorities including R.A. Jefferson, inventor of the GUS assay
  • Illustrated with color
  • GUS detection by fluorometric, spectrophotometric, and histochemical methods
  • Fast, automated assays

Readership

Graduate students and researchers in molecular biology, biotechnology, molecular genetics, biochemistry, animal cell biology and developmental biology, and plant science

Table of Contents

  • (Section Headings): The GUS Reporter Gene System. The GUS Assay. Histochemical Detection of GUS. Applications of GUS to Plant Genetic Analysis. Applications of GUS to Animal Genetic Anaylsis. Appendices. Index.

Product details

  • No. of pages: 221
  • Language: English
  • Copyright: © Academic Press 1992
  • Published: March 10, 1992
  • Imprint: Academic Press
  • eBook ISBN: 9780323137645

About the Editor

Sean Gallagher

Affiliations and Expertise

Hoefer Scientific Instruments, San Francisco, California, U.S.A.

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