PART I - Functional Genomics
1.Analysis of Gene Function using DNA Microarrays
Andrew P. Capaldi
2.An introduction to microarray data analysis and visualization
Gregg B. Whitworth
3. Genome-wide approaches to monitor pre-mRNA splicing
Maki Inada and Jeff Pleiss
4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites
Philippe Lefrançois, Wei Zheng and Michael Snyder
5. Genome-wide mapping of nucleosomes in yeast
Oliver J. Rando
6. Genome-wide translational profiling by ribosome footprinting
Nicholas T. Ingolia
PART II - Systematic Genetic Analysis
7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe
Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone
8. Making temperature-sensitive mutants
Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter
9. Quantitative Genetic Interaction Mapping using the E-MAP Approach
Sean R. Collins, Assen Roguev and Nevan J. Krogan
10. Exploring gene function and drug action using chemogenomic dosage assays
Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow
PART III - Proteomics
11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry
Tobias C. Walther, Jesper V. Olsen and Matthias Mann
12. High-quality binary interactome mapping
Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun
13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics
This fully updated edition of the best-selling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.
Provides up-to-date methods necessary to study genes in yeast.
Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations.
This volume serves as an essential reference for any beginning or experienced researcher in the field.
Biochemists, geneticists, molecular biologists, cell biologists, and microbiologists.
- No. of pages:
- © Academic Press 2010
- 16th March 2010
- Academic Press
- eBook ISBN:
- Paperback ISBN:
Praise for the first edition:
"...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers."--Will Prinz, National Institutes of Health for ANALYTICAL BIOCHEMISTRY (April 2003)
University of California, San Francisco, U.S.A.
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, U.S.A.