Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.
- Basic Methods in Yeast Genetics
- Physical and genetic mapping
- Making and recovering mutants
- Cloning and Recombinant DNA Methods
- High-efficiency transformation
- Preparation of yeast artificial chromosome vectors
- Basic Methods of Cell Biology
- Protein targeting assays
- Biochemistry of Gene Expression
- Vectors for regulated expression
- Isolation of labeled and unlabeled DNA, RNA, and protein
Biochemists, geneticists, molecular and cell biologists, microbiologists, biotechnologists, and graduate and postgraduate students in these disciplines.
- No. of pages:
- © Academic Press 1990
- 28th December 1990
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Volume @qu:"This book will be a valuable resource both for beginners and for current practitioners. It should enable newcomers to set up a yeast laboratory and master basic manipulations." @source:--CAMBRIDGE SCIENTIFIC ABSTRACTS @qu:"Because this book covers so much in what is a technique-rich area, no researcher in the field will want to be without it... This is an exceptional book. While it is indeed a guide to many techniques and methods, in the final analysis, it is more than that. It is also a proclamation that yeast biology is now in the main stream." @source:--ASM NEWS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series
University of California, San Francisco, U.S.A.
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, U.S.A.