Guide to Yeast Genetics and Molecular Biology, Volume 194

1st Edition

Print ISBN: 9780121820954
eBook ISBN: 9780080575131
Imprint: Academic Press
Published Date: 28th December 1990
Page Count: 933
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Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.

Key Features

Key Features

  • Basic Methods in Yeast Genetics
  • Physical and genetic mapping
  • Making and recovering mutants
  • Cloning and Recombinant DNA Methods
  • High-efficiency transformation
  • Preparation of yeast artificial chromosome vectors
  • Basic Methods of Cell Biology
  • Immunomicroscopy
  • Protein targeting assays
  • Biochemistry of Gene Expression
  • Vectors for regulated expression
  • Isolation of labeled and unlabeled DNA, RNA, and protein


Biochemists, geneticists, molecular and cell biologists, microbiologists, biotechnologists, and graduate and postgraduate students in these disciplines.

Table of Contents

Basic Methods of Yeast Genetics:

F. Sherman, Getting Started with Yeast.

F. Sherman and J. Hicks, Micromanipulation and Dissection of Asci.

F. Sherman and P. Wakem, Mapping Yeast Genes.

S.L. Gerring, C. Connelly, and P. Hieter, Positional Mapping of Genes by Chromosome Blotting and Chromosome Fragmentation.

G.F. Sprague, Jr., Assay of Yeast Mating Reaction.

Y. Kassir and G. Simchen, Monitoring Meiosis and Sporulation in Saccharomyces cerevisiae.

R.E. Esposito, M. Dresser, and M. Breitenbach, Identifying Sporulation Genes, Visualizing Synaptonemal Complexes, and Large-Scale Spore and Spore Wall Purification.

I. Herskowitz and R.E. Jensen, Putting the HO Gene to Work: Practical Uses for Mating-Type Switching.

B. Rockmill, E.J. Lambie, and G.S. Roeder, Spore Enrichment.

T.D. Fox, L.S. Folley, J.J. Mulero, T.W. McMullin, P.E. Thorsness, L.O. Hedin, and M.C. Costanzo, Analysis and Manipulation of Yeast Mitochondrial Genes.

Cloning and Recombinant DNA:

P. Philippsen, A. Stotz, and C. Scherf, DNA of Saccharomyces cerevisiae.

D.M. Becker and L. Guarente, High-Efficiency Transformation of Yeast by Electroporation.

J.A. Heinemann and G.F. Sprague, Jr., Transmission of Plasmid DNA to Yeast by Conjugation with Bacteria.

M.D. Rose and J.R. Broach, Cloning Genes by Complementation in Yeast.

R.A. Young and R.W. Davis, Gene Isolation with ~glgt11 System.

J. Rine, Gene Overexpression in Studies of Saccharomyces cerevisiae.

D.T. Burke and M.V. Olson, Preparation of Clone Libraries in Yeast Artificial-Chromosome Vectors.



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© Academic Press 1990
Academic Press
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@from:Praise for the Volume @qu:"This book will be a valuable resource both for beginners and for current practitioners. It should enable newcomers to set up a yeast laboratory and master basic manipulations." @source:--CAMBRIDGE SCIENTIFIC ABSTRACTS @qu:"Because this book covers so much in what is a technique-rich area, no researcher in the field will want to be without it... This is an exceptional book. While it is indeed a guide to many techniques and methods, in the final analysis, it is more than that. It is also a proclamation that yeast biology is now in the main stream." @source:--ASM NEWS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series