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Guide to Techniques in Mouse Development - 1st Edition - ISBN: 9780121821265, 9780080883380

Guide to Techniques in Mouse Development, Volume 225

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Melvin DePamphilis
eBook ISBN: 9780080883380
Imprint: Academic Press
Published Date: 20th August 1993
Page Count: 1021


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Table of Contents

General Methodology: L.M. Silver, Recordkeeping and Database Analysis of Breeding Colonies. P.A. Flecknell, Anesthesia and Perioperative Care. Germ Cells and Embryos: J.E. Cooke, I. Godin, C. French-Constant, J. Heasman, and C.C. Wylie, Culture and Manipulation of Primordial Germ Cells. M. Buehr and A.McLaren, Isolation and Culture of Primordial Germ Cells. J.J. Eppig and E.E. Telfer, Isolation and Culture of Oocytes. A.R. Bellvé, Purification, Culture, and Fractionation of Spermatogenic Cells. A.R. Bellvé, W. Zheng, and Y.S. Martinova, Recovery, Capacitation, Acrosome Reaction, and Fractionation of Sperm. R.A. Cardullo and H.M. Florman, Strategies and Methods for Evaluating Acrosome Reaction. J.A. Lawits and J.D. Biggers, Culture of Preimplantation Embryos. K. Sturm and P.P.L. Tam, Isolation and Culture of Whole Postimplantation Embryos and Germ Layer Derivatives. P.P.L. Tam, Histogenetic Potency of Embryonic Tissues in Ectopic Sites. Fertilization: J.W. Gordon, Micromanipulation of Gametes and Embryos. L.R. Fraser, In Vitro Capacitation and Fertilization. J.D. Bleil, In Vitro Fertilization. Gene Expression: Messenger RNA: K.B. Clegg and L. Pikó, Radioisotope Labeling of UTP and ATP Pools and Quantitative Analysis of RNA Synthesis. K.D. Taylor and Lajos Pikó, Quantitative Analysis of Specific Messenger RNAs by Hybridization Using RNA Probes R.A. Kinloch, R.J. Roller, and P.M. Wassarman, Quantitative Analysis of Specific Messenger RNAs by Ribonuclease Protection. M.Y. Arcellana-Panlilio and G.A. Schultz, Analysis of Messenger RNA. M.F. Gaudette, Q.P. Cao, and W.R. Crain, Quantitative Analysis of Specific Messenger RNAs in Small Numbers of Preimplantation Embryos. J. Singer-Sam and A.D. Riggs, Quantitative Analysis of Messenger RNA Levels: Reverse Transcription-Polymerase Chain Reaction Single Nucleotide Primer Extension Assay. F.J. Sallés, W.G. Richards, J. Huarte, J.-D. Vassali, and S. Strickland, Microinjecting Antisense Sequences into Oocytes. D.G. Wilkinson and M.A. Nieto, Detection of Messenger RNA by in situ Hybridization to Tissue Sections and Whole Mounts. R.A. Conlon and B.G. Herrmann, Detection of Messenger RNA by in situ Hybridization to Postimplantation Embryo Whole Mounts. D. Sassoon and N. Rosenthal, Detection of Messenger RNA by in situ Hybridization. Gene Expression: Reporter Genes: M. Miranda and M.L. DePamphilis, Preparation of Injection Pipettes. M. Miranda, S. Majumder, M. Wiekowski, and M.L. DePamphilis, Application of Firefly Luciferase to Preimplantation Development. M. Vernet, C. Bonnerot, P. Briand, and J.-F. Nicolas, Application of LacZ Gene Fusions to Preimplantation Development. C. Bonnerot and J.-F. Nicolas, Application of LacZ Gene Fusions to Postimplantation Development. Gene Expression: Proteins: K.E. Latham, J.I. Garrels, and D. Solter, Two-Dimensional Gel Analysis of Protein Synthesis. M. Wiekowski and M.L. DePamphilis, One-Dimensional Gel Analysis of Histone Synthesis. C.A. Davis, Whole-Mount Immunohistochemistry. C. Simerly and G. Schatten, Techniques for Localization of Specific Molecules in Oocytes and Embryos. Gene Expression: Methylation: M. Zuccotti, M. Grant, and M. Monk, Polymerase Chain Reaction for the Detection of Methylation of a Specific CpG Site in the G6pd Gene of Mouse Embryos. G.P. Pfeifer, J. Singer-Sam, and A.D. Riggs, Analysis of Methylation and Chromatin Structure. Gene Identification: J.L. Rothstein, D. Johnson, J. Jessee, J. Skowronski, J.A. DeLoia, D. Solter, and B.B. Knowles, Construction of Primary and Subtracted cDNA Libraries from Early Embryos. G. Brady and N.N. Iscove, Construction of cDNA Libraries from Single Cells. Z. Larin, A.P. Monaco, S. Meier-Ewert, and H. Lehrach, Construction and Characterization of Yeast Artificial Chromosome Libraries. R.D. Cox, S. Meier-Ewert, M. Ross, Z. Larin, A.P. Monaco, and H. Lehrach, Genome Mapping and Cloning of Mutations Using Yeast Artificial Chromosomes. B.J. Gavin and A.P. McMahon, Cloning Developmentally Regulated Gene Families. D.P. Hill and W. Wurst, Screening for Novel Pattern Formation Genes Using Gene Trap Approaches. G. Friedrich and P. Soriano, Insertional Mutagenesis by Retroviruses and Promoter Traps in Embryonic Stem Cells. R.D. Sheppard and L.M. Silver, Two-Dimensional Gel Analysis of Repetitive DNA Families. Nuclear Transplantation: K.E. Latham and D. Solter, Transfer of Nuclei to Eggs and Embryos. S.C. Barton and M.A. Surani, Nuclear Transplantation. Transgenic Animals: Pronuclear Transplantation: J.W. Gordon, Production of Transgenic Mice. J.R. Mann and A.P. McMahon, Factors Influencing Efficient Production of Transgenic Mice. J.R. Mann, Surgical Techniques in Production of Transgenic Mice. M. Gendron-Maguire and T. Gridley, Identification of Transgenic Mice. Transgenic Animals: Embryonic Stem Cells and Gene Targeting: S.J. Abbondanzo, I. Gadi, and C.L. Stewart, Derivation of Embryonic Stem Cell Lines. C.L. Stewart, Production of Chimeras between Embryonic Stem Cells and Embryos. R. Ramírez-Solis, A.C. Davis, and A. Bradley, Gene Targeting in Embryonic Stem Cells. F. Köntgen and C.L. Stewart, Simple Screening Procedure to Detect Gene Targeting Events in Embryonic Stem Cells. B. Sauer, Manipulation of Transgene by Site-Specific Recombination: Use of Cre Recombinase. M.V. Wiles, Embryonic Stem Cell Differentiation In Vitro. K.J. McLaughlin, Production of Tetraploid Embryos by Electrofusion. Lineage Analysis: C.L. Cepko, E.F. Ryder, C.P. Austin, C. Walsh, and D.M. Fekete, Lineage Analysis Using Retroviral Vectors. Author Index. Subject Index.


This volume provides, for the first time, an extensive compilation of those state-of-the-art methods that have revolutionized the use of mice as an experimental system. This volume is a unique source for detailed descriptions to be used in research by both experienced and novice researchers - Germ cells and embryos; Fertilization; Gene expression: messenger RNA, reporter genes, proteins, methylation; Gene identification; Nuclear transplantation; Transgenic animals: pronuclear injection, embryonic stem cells, and gene targeting; Lineage analysis.

Key Features

@introbul:Key Features @bul:* Contains the most up-to-date methodology as applied to mouse development

  • Focuses on molecular approaches to experimental mouse development
  • The first comprehensive source for molecular biological methods in mouse development
  • Many of the methods have never appeared before


Molecular, cell, and developmental biologists, geneticists, biochemists, biotechnologists, and graduate and postgraduate students in these disciplines.


No. of pages:
© Academic Press 1993
20th August 1993
Academic Press
eBook ISBN:


@from:Praise for the Volume @qu:"Each section is divided into comprehensive subsections with introduction methodology, analysis of data. The text is clear and the methods are well presented in an itemized way. It even gives the source of the chemicals and how to store them. This book is highly recommended for anyone interested in molecular analysis of gene development in mice as well as for senior and even junior researchers operating in the general field of molecular biology." @source:--CELLULAR & MOLECULAR BIOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Melvin DePamphilis

Affiliations and Expertise

Roche Institute of Molecular Biology, Department of Cell and Developmental Biology, Roche Research Center, Nutley, New Jersey, U.S.A.