Guide to Techniques in Mouse Development, Part B, Volume 477

Section I. Transgenesis

1. Lentivirus transgenesis

Alexander Pfeifer and Katrin Zimmermann

2. Germline modification using mouse spermatogonial stem cells

Mito Kanatsu-Shinohara and Takashi Shinohara

3. Embryonic in vivo electroporation in the mouse

Tetsuichiro Saito

Section II. Transposons

4. Current applications of transposons in mouse genetics

Adam J. Dupuy

5. Functional genomics in the mouse using the Sleeping Beauty transposon system

Kyoji Horie, Chikara Kokubu, and Junji Takeda

6. The use of DNA transposons for cancer gene discovery in mice

George Vassiliou, Roland Rad, and Allan Bradley

 

Section III. Recombinases

7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2

Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Berhardt, and A. Francis Stewart

8. A recombineering pipeline to make conditional targeting constructs

Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, and A. Francis Stewart

9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria

M. David Stewart and Richard R. Behringer

10.Genetic fate mapping using site-specific recombinases

Emilie Legué and Alexandra L. Joyner

11. Mapping cell fate and function using recombinase-based intersectional strategies

Susan M. Dymecki, Russell S. Ray, and Jun C. Kim

 

Section IV. Mutagenesis

12. Genome-wide forward genetic screens in mouse ES cells

Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, and Allan Bradley

13. Gene trap mutagenesis in the mouse

Roland H. Friedel and Philippe Soriano

14. A wider context for gene trap mutagenesis

Joshua M. Brickman, Anestis Tsakiridis, Christine To, and William L. Stanford

15. Mouse mutagenesis with the chemical supermutagen ENU

Frank J. Probst and Monica J. Justice

16. Phenotype-driven mouse ENU mutagenesis screens

Tamara Caspary

17. Using ENU mutagenesis for phenotype-driven analysis of the mouse.

Rolf W. Stottmann and David R. Beier

 

Section V. Gene Knockdowns

18. Exploration of self-renewal and pluripotency in ES cells using RNAi

Christoph Schaniel, Dung-Fang Lee, and Ihor R. Lemischka

19. Transgenic RNAi applications in the mouse

Jost Seibler and Frieder Schwenk

20. Gene knockdown in the mouse through RNAi

Aljoscha Kleinhammer, Wolfgang Wurst, and Ralf Kühn

21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice

Christopher S. Raymond, Lei Zhu, and Myung K. Shin

22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription

Kai Schönig, Hermann Bujard, and Manfred Gossen

Section VI. Gene Expression Profiling

23. Gene expression profiling of mouse oocytes and preimplantation embryos

Francesca E. Duncan and Richard M. Schultz

24. Interrogating the transcriptome of oocytes and preimplantation embryos

Anne E. Peaston, Joel Graber, Barbara B. Knowles, and Wilhelmine N. de Vries

25. Gene expression profiling of mouse embryos with microarrays

Alexei A. Sharov, Yulan Piao, and Minoru S. H. Ko

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

• Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
• Comprehensively covers:
• new techniques for the cryopreservation of gametes and embryos
• production of transgenic and null (knockout) animals (use of ES cells)
• generation of conditional/inducible mutant animals, use of gene-trap mutagenesis
• analysis of allele-specific expression, use of new reporter constructs
• humanizing of transgenic animals
• transcript profiling of mouse development
• imaging of mouse development
• rederivation of animals and use of mouse genomics

Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.