Guide to Techniques in Mouse Development, Part B, Volume 477

Section I. Transgenesis

1. Lentivirus transgenesis

Alexander Pfeifer and Katrin Zimmermann

2. Germline modification using mouse spermatogonial stem cells

Mito Kanatsu-Shinohara and Takashi Shinohara

3. Embryonic in vivo electroporation in the mouse

Tetsuichiro Saito

4. Current applications of transposons in mouse genetics

Adam J. Dupuy

5. Functional genomics in the mouse using the Sleeping Beauty transposon system

Kyoji Horie, Chikara Kokubu, and Junji Takeda

6. The use of DNA transposons for cancer gene discovery in mice

George Vassiliou, Roland Rad, and Allan Bradley

7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2

Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Berhardt, and A. Francis Stewart

8. A recombineering pipeline to make conditional targeting constructs

Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, and A. Francis Stewart

9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria

M. David Stewart and Richard R. Behringer

10.Genetic fate mapping using site-specific recombinases

Emilie Legué and Alexandra L. Joyner

11. Mapping cell fate and function using recombinase-based intersectional strategies

Susan M. Dymecki, Russell S. Ray, and Jun C. Kim

12. Genome-wide forward genetic screens in mouse ES cells

Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, and Allan Bradley

13. Gene trap mutagenesis in th

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

• Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
• Comprehensively covers:
• new techniques for the cryopreservation of gametes and embryos
• production of transgenic and null (knockout) animals (use of ES cells)
• generation of conditional/inducible mutant animals, use of gene-trap mutagenesis
• analysis of allele-specific expression, use of new reporter constructs
• humanizing of transgenic animals
• transcript profiling of mouse development
• imaging of mouse development
• rederivation of animals and use of mouse genomics

Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.