Guide to Techniques in Mouse Development, Part B, Volume 477
1st Edition
Mouse Molecular Genetics
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Table of Contents
Section I. Transgenesis
1. Lentivirus transgenesis
Alexander Pfeifer and Katrin Zimmermann
2. Germline modification using mouse spermatogonial stem cells
Mito Kanatsu-Shinohara and Takashi Shinohara
3. Embryonic in vivo electroporation in the mouse
Tetsuichiro Saito
Section II. Transposons
4. Current applications of transposons in mouse genetics
Adam J. Dupuy
5. Functional genomics in the mouse using the Sleeping Beauty transposon system
Kyoji Horie, Chikara Kokubu, and Junji Takeda
6. The use of DNA transposons for cancer gene discovery in mice
George Vassiliou, Roland Rad, and Allan Bradley
Section III. Recombinases
7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2
Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Berhardt, and A. Francis Stewart
8. A recombineering pipeline to make conditional targeting constructs
Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, and A. Francis Stewart
9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria
M. David Stewart and Richard R. Behringer
10.Genetic fate mapping using site-specific recombinases
Emilie Legué and Alexandra L. Joyner
11. Mapping cell fate and function using recombinase-based intersectional strategies
Susan M. Dymecki, Russell S. Ray, and Jun C. Kim
Section IV. Mutagenesis
12. Genome-wide forward genetic screens in mouse ES cells
Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, and Allan Bradley
13. Gene trap mutagenesis in the mouse
Roland H. Friedel and Philippe Soriano
14. A wider context for gene trap mutagenesis
Joshua M. Brickman, Anestis Tsakiridis, Christine To, and William L. Stanford
15. Mouse mutagenesis with the chemical supermutagen ENU
Frank J. Probst and Monica J. Justice
16. Phenotype-driven mouse ENU mutagenesis screens
Tamara Caspary
17. Using ENU mutagenesis for phenotype-driven analysis of the mouse.
Rolf W. Stottmann and David R. Beier
Section V. Gene Knockdowns
18. Exploration of self-renewal and pluripotency in ES cells using RNAi
Christoph Schaniel, Dung-Fang Lee, and Ihor R. Lemischka
19. Transgenic RNAi applications in the mouse
Jost Seibler and Frieder Schwenk
20. Gene knockdown in the mouse through RNAi
Aljoscha Kleinhammer, Wolfgang Wurst, and Ralf Kühn
21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice
Christopher S. Raymond, Lei Zhu, and Myung K. Shin
22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription
Kai Schönig, Hermann Bujard, and Manfred Gossen
Section VI. Gene Expression Profiling
23. Gene expression profiling of mouse oocytes and preimplantation embryos
Francesca E. Duncan and Richard M. Schultz
24. Interrogating the transcriptome of oocytes and preimplantation embryos
Anne E. Peaston, Joel Graber, Barbara B. Knowles, and Wilhelmine N. de Vries
25. Gene expression profiling of mouse embryos with microarrays
Alexei A. Sharov, Yulan Piao, and Minoru S. H. Ko
Description
This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.
Key Features
- Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
- Comprehensively covers:
- new techniques for the cryopreservation of gametes and embryos
- production of transgenic and null (knockout) animals (use of ES cells)
- generation of conditional/inducible mutant animals, use of gene-trap mutagenesis
- analysis of allele-specific expression, use of new reporter constructs
- humanizing of transgenic animals
- transcript profiling of mouse development
- imaging of mouse development
- rederivation of animals and use of mouse genomics
Readership
Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.
Details
- No. of pages:
- 628
- Language:
- English
- Copyright:
- © Academic Press 2010
- Published:
- 27th August 2010
- Imprint:
- Academic Press
- Hardcover ISBN:
- 9780123848802
- eBook ISBN:
- 9780123848819
Ratings and Reviews
About the Editors
Paul Wassarman
Affiliations and Expertise
Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA, Tel: +1 212 241 8616, Fax: +1 212 427 7532, Email:
Philippe Soriano
Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA
Affiliations and Expertise
Icahn School of Medicine at Mount Sinai, NY, USA
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