This volume provides, for the first time, an extensive compilation of those state-of-the-art methods that have revolutionized the use of mice as an experimental system. This volume is a unique source for detailed descriptions to be used in research by both experienced and novice researchers - Germ cells and embryos; Fertilization; Gene expression: messenger RNA, reporter genes, proteins, methylation; Gene identification; Nuclear transplantation; Transgenic animals: pronuclear injection, embryonic stem cells, and gene targeting; Lineage analysis.

Key Features

@introbul:Key Features @bul:* Contains the most up-to-date methodology as applied to mouse development * Focuses on molecular approaches to experimental mouse development * The first comprehensive source for molecular biological methods in mouse development * Many of the methods have never appeared before


Molecular, cell, and developmental biologists, geneticists, biochemists, biotechnologists, and graduate and postgraduate students in these disciplines.

Table of Contents

General Methodology: L.M. Silver, Recordkeeping and Database Analysis of Breeding Colonies. P.A. Flecknell, Anesthesia and Perioperative Care. Germ Cells and Embryos: J.E. Cooke, I. Godin, C. French-Constant, J. Heasman, and C.C. Wylie, Culture and Manipulation of Primordial Germ Cells. M. Buehr and A.McLaren, Isolation and Culture of Primordial Germ Cells. J.J. Eppig and E.E. Telfer, Isolation and Culture of Oocytes. A.R. Bellvé, Purification, Culture, and Fractionation of Spermatogenic Cells. A.R. Bellvé, W. Zheng, and Y.S. Martinova, Recovery, Capacitation, Acrosome Reaction, and Fractionation of Sperm. R.A. Cardullo and H.M. Florman, Strategies and Methods for Evaluating Acrosome Reaction. J.A. Lawits and J.D. Biggers, Culture of Preimplantation Embryos. K. Sturm and P.P.L. Tam, Isolation and Culture of Whole Postimplantation Embryos and Germ Layer Derivatives. P.P.L. Tam, Histogenetic Potency of Embryonic Tissues in Ectopic Sites. Fertilization: J.W. Gordon, Micromanipulation of Gametes and Embryos. L.R. Fraser, In Vitro Capacitation and Fertilization. J.D. Bleil, In Vitro Fertilization. Gene Expression: Messenger RNA: K.B. Clegg and L. Pikó, Radioisotope Labeling of UTP and ATP Pools and Quantitative Analysis of RNA Synthesis. K.D. Taylor and Lajos Pikó, Quantitative Analysis of Specific Messenger RNAs by Hybridization Using RNA Probes R.A. Kinloch, R.J. Roller, and P.M. Wassarman, Quantitative Analysis of Specific Messenger RNAs by Ribonuclease Protection. M.Y.


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© 1993
Academic Press
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Electronic ISBN:


@from:Praise for the Volume @qu:"Each section is divided into comprehensive subsections with introduction methodology, analysis of data. The text is clear and the methods are well presented in an itemized way. It even gives the source of the chemicals and how to store them. This book is highly recommended for anyone interested in molecular analysis of gene development in mice as well as for senior and even junior researchers operating in the general field of molecular biology." @source:--CELLULAR & MOLECULAR BIOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A seri