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Guide to Research Techniques in Neuroscience, Third Edition provides a diverse spectrum of neuroscience techniques, their execution, and the benefits and limitations of each method. It is essential for PIs and experienced researchers who want to expand their technical expertise, making it even more crucial for new researchers, students and post-docs. Many new techniques, including in vivo calcium imaging, fiber photometry, microendoscopy, and of course, genome engineering via CRISPR-Cas9, are now much more prominent and discussed in detail in this new edition. In addition, multiple illustrations and figures have been updated, ensuring the book is the most current reflection of the field.
- Provides a "hands-on" approach for graduate students, postdocs or anyone new to neuroscience
- Expands on techniques in previous editions and covers many new techniques developed since, including in vivo calcium imaging, fiber photometry and microendoscopy
- Features a new section on genome engineering via CRISPR-Cas9
- Incorporates updates to every chapter and multiple figures
- Includes walk-through boxes that guide readers step-by-step through experiments
- Covers cultured brain spheroids, tissue clearing and Drop-Seq
- Presents the most accurate reflection of the current state of the neuroscience field
Neuroscience graduate students, post-doctoral fellows, experienced researchers new to neuroscience, and senior researchers that want technique updates
- Whole Brain Imaging
2. Animal Behavior
3. Stereotaxic Surgeries and in vivo Techniques
6. Visualizing Nervous System Structure
7. Visualizing Nervous System Function
8. Manipulating Neural Activity
9. Identifying Genes and Proteins of Interest
10. Molecular Cloning and Recombinant DNA Technology
11. Gene Delivery Strategies
12. Making and using transgenic organisms
13. Manipulating endogenous genes
14. Cell culture techniques
15. Biochemical assays and intracellular signaling
- No. of pages:
- © Academic Press 2022
- 1st February 2022
- Academic Press
- Paperback ISBN:
Matt Carter is Associate Professor of Biology at Williams College where he teaches courses in neuroscience and physiology. His research focuses on how the brain regulates food intake and sleep, and is funded by grants from the National Institutes of Health and National Science Foundation. In addition to scientific publications, he is also the author of Designing Science Presentations (Academic Press). He is a recipient of the Walter Gores Award for Excellence in Teaching from Stanford University and the Nelson Bushnell Prize for Teaching and Writing from Williams College. He lives in Williamstown with his wife and three children.
Assistant Professor of Biology, Williams College, Williamstown, MA, USA
Rachel Essner is a PhD candidate in Neuroscience at Harvard University and a National Science Foundation Graduate Research Fellow. She received a B.A. in Biology from Williams College in 2016, where she was awarded the Erastus C. Benedict prize in Biology. Her current research focuses on how the brain processes and regulates sensory signals from within the body.
Harvard University, USA
Nitsan Goldstein is a neuroscience Ph.D. candidate at the University of Pennsylvania. Her thesis research focuses on gut-brain communication and how the brain regulates innate behaviors. Nitsan received her B.A. in Biology and Psychology from Williams College where she received the Erastus C. Benedict prize in Biology. She is currently supported by a National Science Foundation Graduate Research Fellowship award.
University of Pennsylvania, USA
Manasi Iyer is a Ph.D. Candidate and Stanford Graduate Fellow in Science and Engineering in Dr. Brad Zuchero’s lab at Stanford University. In the Zuchero lab, Manasi is studying the molecular and cellular mechanisms that underlie developmental myelin formation and myelin plasticity. When she is not in lab, Manasi spends her time teaching undergraduate courses such as Foundational Topics in Neuroscience and working with NeuWrite West as an editor and blog manager. She is also passionate about early child education.
Stanford University, Standford, California, USA
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