This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.
@introbul:Key Features @bul:* Comprehensive protocols compiled specifically for this volume
- Among the topics covered: @subbul:* Genomic cloning
- Preparation and characterization of mRNA cDNA cloning
- Screening libraries
- Identification and characterization of clones
- Methods written by 87 experts
- Editors' notes, overviews, and extensive cross-referencing
- Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility
- A problem-solving quantitative approach
- Emphasis on strategies for choosing alternative methods
- No. of pages:
- © Academic Press 1987
- 14th September 1987
- Academic Press
- eBook ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA
National Cancer Institute, National Institutes of Health, Bethesda, Maryland, U.S.A.
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, U.S.A.
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