Description

This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.

Key Features

@introbul:Key Features @bul:* Comprehensive protocols compiled specifically for this volume * Among the topics covered: @subbul:* Genomic cloning * Preparation and characterization of mRNA cDNA cloning * Screening libraries * Identification and characterization of clones * Methods written by 87 experts * Editors' notes, overviews, and extensive cross-referencing * Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility * A problem-solving quantitative approach * Emphasis on strategies for choosing alternative methods

Details

No. of pages:
812
Language:
English
Copyright:
© 1987
Published:
Imprint:
Academic Press
Print ISBN:
9780121820534
Electronic ISBN:
9780080882697

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY