Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.
Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques.
@introbul:Key Features @bul:* Covers fundamentals of electroporation and electrofusion in detail
- Molecular events
- Gives extensive practical information
- The latest applications
- Controlling parameters to maximize efficiency
- Available instrumentation
- Presents applications of electroporation and electrofusion in current research situations
- State-of-the-art modifications to electrical pulses and generators
- Application of electroporation and electrofusion to unique, alternative cell and tissue types
- Gives straightforward, detailed, easy-to-follow protocols for
- Formation of human hybridomas
- Introduction of genetic material into plant cells and pollen
- Transfection of mammalian cells
- Transformation of bacteria, plants, and yeast
- Production of altered embryos
- Optimization of electroporation by using reporter genes
- Comprehensive and up-to-date
- Convenient bench-top format
- Approximately 125 illustrations complement the text
- Complete references with article titles
- Written by leading authorities in electroporation and electrofusion
Researchers in molecular and cell biology, biotechnology, microbiology, immunology, plant molecular biology, biochemistry, biophysics, and developmental biology.
D.C. Chang, B.M. Chassy, J.A. Saunders, and A.E. Sowers, Overview of Electroporation and Electrofusion.
Mechanisms and Fundamental Processes in Electroporation and Electrofusion:
D.C. Chang, Structure and Dynamics of Electric Field-Induced Membrane Pores as Revealed by Rapid-Freezing Electron Microscopy.
K. Kinosita, M. Hibino, H. Itoh, M. Shigemori, K. Hirano, Y. Kirino, and T. Hayakawa, Events of Membrane Electroporation Visualized on a Time Scale from Microseconds to Seconds.
T.Y. Tsong, Time Sequence of Molecular Events in Electroporation.
L.V. Chernomordik, Electropores in Lipid Bilayers and Cell Membranes.
E. Neumann, A. Sprafke, E. Boldt, and H. Wolf, Biophysical Considerations of Membrane Electroporation.
J.C. Weaver and A. Barnett, Progress Toward a Theoretical Model for Electroporation Mechanism: Membrane Electrical Behavior and Molecular Transport.
A.E. Sowers, Mechanisms of Electroporation and Electrofusion.
J. Teissi*aae and M.-P. Rols, Interfacial Membrane Alteration Associated with Electropermeabilization and Electrofusion.
D.S. Dimitrov, Membrane Fusion Kinetics.
S.W. Hui and D.A. Stenger, Effects of Intercellular Forces on Electrofusion.
Q. Zheng and D.C. Chang, Dynamics of Cytoskeletal Reorganization in CV-1 Cells during Electrofusion.
Applications of Electroporation and Electrofusion in Current Research:
H. Potter and S.W.F. Cooke, Gene Transfer into Adherent Cells Growing on Microbeads.
L.H. Reid and O. Smithies, Gene Targeting and Electroporation.
J.A. Saunders, B.F. Matthews, and S.L. Van Wert, Pollen Electrotransformation for Gene Transfer in Plants.<BR id="CRL
- No. of pages:
- © Academic Press 1991
- 11th December 1991
- Academic Press
- eBook ISBN:
- Paperback ISBN:
Baylor College of Medicine, Houston, Texas, U.S.A. and Hong Kong University of Science and Technology, Kowloon, Hong Kong
University of Illinois at Urbana-Champaign, Urbana, U.S.A.
Beltsville Agricultural Research Center, U.S.D.A., Beltsville, Maryland, U.S.A.
School of Medicine, University of Maryland, Baltimore, U.S.A.
@qu:"The protocols are well presented... Excellent instructions for optimizing the procedures on different cell types... Anyone who wants to use electroporation or electrofusion in anything but a routine fashion will find this book very useful." @source:--ANALYTICAL BIOCHEMISTRY