Growth, Nutrition, and Metabolism of Cells in Culture, Volume 2, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume deals with specialized mammalian, plant, and invertebrate cell systems and techniques.
The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on the use of perfusion systems in cell and tissue culture; and the cultivation of muscle tissue, nerve tissue, and hematopoietic cells. Subsequent chapters discuss the use of cell culture to study mechanisms of hormone action; the cultivation of mammalian embryos; cultivation of cells from poikilothermie vertebrates; and the cultivation of arthropod cells and plant cells.
This book will be valuable resource for investigators who routinely use cell culture techniques, as well as students and individuals in associated areas of cell and molecular biology.
List of Contributors Preface Contents of Volume I
- General Introduction Text References
- Use of Perfusion Systems for Growth of Cell and Tissue Cultures I. Introduction II. Stationary Culture Perfusion Systems III. Rolling Bottle Perfusion Culture Systems IV. Suspension Culture Perfusion Systems V. Performance Aspects and Applications of Perfusion Systems VI. Conclusions References
- Cultivation of Muscle Tissue
II. Culture Methods
III. Experimental Analysis of Myogenesis In Vitro
- Cultivation of Nerve Tissue I. Historical Perspective II. Methods III. Nutritional and Metabolic Requirements for Maintenance or Growth, and Metabolic Characteristics of Nervous Tissue in Culture IV. Experimental Neurophathology in Culture V. Conclusion References
- Cultivation of Hematopoietic Cells I. Introduction II. Early Studies on Culture of Lymphoid Cells III. Sources of Established Lines IV. Factors Affecting Isolation V. Cultural Characteristics VI. Nutritional and Metabolic Requirements VII. Products of Hemic Cell Lines VIII. Other Studies IX. Comment References
- Hormone Synthesis and Function In Vitro I. Introduction II. Energy Metabolism by Hormone-Secreting Endocrine Tissue III. Culture Methods IV. Hormone-Secreting Tissues and Their Actions In Vitro V. Physiological Considerations in Hormone Action References
- Cultivation of the Mammalian Embryo I. Introduction II. Techniques III. Physical Environment IV. Nutritional Substrates V. Typical Media VI. Assessing Results VII. Conclusions References
- Cultivation of Cells from Poikilothermic Vertebrates I. Introduction II. Nutritional Requirements for Cell Culture III. Temperature Requirements for Cell Cul
- No. of pages:
- © Academic Press 1972
- 1st January 1972
- Academic Press
- eBook ISBN: