Growth, Nutrition, and Metabolism of Cells In Culture V2

1st Edition - January 1, 1972
Editor: George Rothblat
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 4 9 5 3 - 2

Growth, Nutrition, and Metabolism of Cells in Culture, Volume 2, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The… Read more

Growth, Nutrition, and Metabolism of Cells In Culture V2

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Growth, Nutrition, and Metabolism of Cells in Culture, Volume 2, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume deals with specialized mammalian, plant, and invertebrate cell systems and techniques. The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on the use of perfusion systems in cell and tissue culture; and the cultivation of muscle tissue, nerve tissue, and hematopoietic cells. Subsequent chapters discuss the use of cell culture to study mechanisms of hormone action; the cultivation of mammalian embryos; cultivation of cells from poikilothermie vertebrates; and the cultivation of arthropod cells and plant cells. This book will be valuable resource for investigators who routinely use cell culture techniques, as well as students and individuals in associated areas of cell and molecular biology.

List of Contributors

Preface

Contents of Volume I

1. General Introduction

Text

References

2. Use of Perfusion Systems for Growth of Cell and Tissue Cultures

I. Introduction

II. Stationary Culture Perfusion Systems

III. Rolling Bottle Perfusion Culture Systems

IV. Suspension Culture Perfusion Systems

V. Performance Aspects and Applications of Perfusion Systems

VI. Conclusions

References

3. Cultivation of Muscle Tissue

I. Introduction

II. Culture Methods

III. Experimental Analysis of Myogenesis In Vitro

References

4. Cultivation of Nerve Tissue

I. Historical Perspective

II. Methods

III. Nutritional and Metabolic Requirements for Maintenance or Growth, and Metabolic Characteristics of Nervous Tissue in Culture

IV. Experimental Neurophathology in Culture

V. Conclusion

References

5. Cultivation of Hematopoietic Cells

I. Introduction

II. Early Studies on Culture of Lymphoid Cells

III. Sources of Established Lines

IV. Factors Affecting Isolation

V. Cultural Characteristics

VI. Nutritional and Metabolic Requirements

VII. Products of Hemic Cell Lines

VIII. Other Studies

IX. Comment

References

6. Hormone Synthesis and Function In Vitro

I. Introduction

II. Energy Metabolism by Hormone-Secreting Endocrine Tissue

III. Culture Methods

IV. Hormone-Secreting Tissues and Their Actions In Vitro

V. Physiological Considerations in Hormone Action

References

7. Cultivation of the Mammalian Embryo

I. Introduction

II. Techniques

III. Physical Environment

IV. Nutritional Substrates

V. Typical Media

VI. Assessing Results

VII. Conclusions

References

8. Cultivation of Cells from Poikilothermic Vertebrates

I. Introduction

II. Nutritional Requirements for Cell Culture

III. Temperature Requirements for Cell Culture

IV. Biochemical Studies of Poikilothermic Vertebrate Cells

V. Summary

References

9. Cultivation of Arthropod Cells

I. Introduction

II. Nutritional Requirements of Arthropod Cells

III. Problems in Media Design

IV. Culture Techniques

V. Applications of Arthropod Tissue Culture

References

10. Cultivation of Plant Cells

I. Introduction

II. Methods

III. Maintenance and Preservation of Cultures

IV. Cultivation of Single Cells

V. Mutants

VI. Protoplasts

VII. Haploid Cell Cultures

VIII. Growth of Plant Cells in Culture

IX. Stability of Cultures

X. Characterization of Plant Cell Lines

XI. Nutrition

XII. Morphogenesis in Plant Cell Cultures

XIII. Metabolism

XIV. Regulation of Metabolism

References

Author Index

Subject Index