Green Fluorescent Protein, Volume 302

1st Edition

Serial Volume Editors: P. Michael Conn
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121822033
eBook ISBN: 9780080884134
Imprint: Academic Press
Published Date: 2nd April 1999
Page Count: 490


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Table of Contents

Monitoring of Physiological Processes:

S. Kupzig, S. San Lee and G. Banting, Membrane Trafficking.

C. Kaether and H.-H. Gerdes, Monitoring of Protein Secretion with Green Fluorescent Protein.

R.M. Hoffman, Green Fluorescent Protein to Visualize Cancer Progression and Metastasis.

X. Zhao, T. Duong, C.-C. Huang, S.R. Kain and X. Li, Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters.

S.R. Kain and J.-T. Ma, Early Detection of Apoptosis with Annexin V-Enhanced Green Fluorescent Protein.

M. Maniak, Green Fluorescent Protein in the Visualization of Particle Uptake and Fluid-Phase Endocytosis.

F. Hanakam and G. Gerisch, Monitoring Intracellular Shuttling of Histidine-rich pH-Sensor Proteins Tagged with Green Fluorescent Protein.

S.R. Cronin and R.Y. Hampton, Measuring Protein Degradation with Green Fluorescent Protein.

G.L. Hager, Studying Nuclear Receptors with Green Fluorescent Protein Fusions.

Localization of Molecules:

M.R. Flory and T.N. Davis Localization of Calmodulin in Budding Yeast and Fission Yeast Using Green Fluorescent Protein.

K.R. Olson and J.B. Olmsted, Analysis of Microtubule Organization and Dynamics in Living Cells Using Green Fluorescent Protein-MAP4 Chimeras.

V. Georget, B. Terouanne, J.-C. Nicholas and C. Sultan, Trafficking of the Androgen Receptor.

C.D. Webb and O. Resnekov, Use of Green Fluorescent Protein for Visualization of Cell-Specific Gene Expression and Subcellular Protein Localization in Bacillus subtilis.

L.S. Barak, J. Zhang, S.S.G. Ferguson and M.G. Caron, Signaling, Desensitization, and Trafficking of G Protein Coupled Receptor


One of the most important recent discoveries in science is the green fluorescent protein, isolated from a bioluminescent jellyfish. The gene that codes for this protein is active in any type of cell, from microbes to humans, producing a "day-glo" dye visible as a bright green light in the microscope. The power of the gene is that by using recombinant DNA technology. GFP can be spliced to any protein, essentially painting that protein green. When such a spliced protein is inside a cell. Further, time-lapse microscopy can be used to examine how that protein and the cellular components that it is part of move within the cell during the cell's life cycle. This Methods in Enzymology volume deals with the utility of green fluorescent protein (GFP). The OVID database (including MEDLINE, Current Contents, and other sources) lists nine references to GFP for the ten-year period 1985-1994. In contrast, in less than four years thereafter, over 500 references are listed, a testament to the rapid growth of interest in this probe. This volume documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in previously published sources. The techniques are described in a context that allows comparisons to other related methodologies-such comparisons are valuable to readers who must adapt existing procedures to new systems. Also, so far as possible, methodologies have been presented in a manner that stresses their general applicability and potential limitations. The volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly await

Key Features

@introbul:Key Features @bul:* Monitoring of Physiological Processes

  • Localization of Molecules
  • Special Uses
  • Mutants
  • Variants of GFP


Biochemists, cell biologists, geneticists, microbiologists, molecular biologists, and biophysicists.


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© Academic Press 1999
Academic Press
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About the Serial Volume Editors

P. Michael Conn Serial Volume Editor

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA