Section 1 - Carbohydrate-Protein Interactions-Physical Measurements
Characterization of specific donor binding to £\1,4-N-acteylhexosaminyltransferase EXTL2 using Isothermal Titration Calorimetry.
NMR Analysis of Carbohydrate-Protein Interactions.
Determination of atomic structure for glycosyltransferase-substrate complex.
Section 2 ¡V Phage Display Library
Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates.
Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan epitopes.
Section 3 - Glycosyltransferase Expression Profiling
Autofluorescent proteins for monitoring the intracellular distribution of glycosyltransferases.
Expression profiling of glycosyltransferases and their related enzymes using in situ hybridization.
Expression profiling of glycosyltransferases using RT-PCR.
Expression profiling of lycosyltransferases and related enzymes using gene micro-arrays.
Section 4 - CHO mutants in Glycan Biosynthesis
Lectin Resistant CHO Glycosylation Mutants.
CHO glycosylation mutants ¡V GPI anchor.
CHO glycosylation mutants ¡Vproteoglycans.
Section 5 - Function of Sulfation
Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Protoglycans).
Measuring the activities of the sulfs, two novel heparin/heparan sulfate endosulfatases.
Determination of chemokine-glycosaminoglycan interaction specificity.
Identification of Proteoglycan-binding Proteins.
Functions of glycans revealed by gene inactivation of L-selectin ligand sulfotransferases in mice.